A study of osteoprotective effect of l-arginine, l-norvaline and rosuvastatin on a model of hypoestrogen-induced osteoporosis in rats

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Abstract

Effect on the microcirculatory bed of the bony tissue is one of promising approaches to treatment of osteoporosis.

Aim. To study anti-osteoporotic properties of endothelioprotectors: L-arginine, L-norvaline and rosuvastatin.

Materials and Methods. Osteoprotective properties of L-arginine, L-norvaline and rosuvastatin, and also of a reference drug– strontium ranelate – were studied on 152 female rats of Wistar line using a model of hypoestrogen-induced osteoporosis. Anti-osteoporotic and endothelioprotective effect of the drugs were evaluated by laser dopplerflowmetry (LDF) of the proximal metaphysis of the femoral bone, morphometry of trabeculae of bone, and also by calculation of the coefficient of endothelial dysfunction.

Results. LDF showed that maximal increase in microcirculation of the proximal metaphysis of the femoral bone, in comparison with animals with untreated osteoporosis (61.52±3.74 perfusion units, PU) was achieved with L-norvaline (115.25±5.36 PU, p<0.001) and rosuvastatin (106.57±5.22 PU, p<0.001), less expressed effect was demonstrated by L-arginine (98.10±4.48 PU, p<0.001) and a reference drug – strontium ranelate (86.49±4.99 PU). A similar tendency was observed in morphometry of trabeculae of bone: in the group with untreated osteoporosis the diameter of trabeculae was 61.68±1.24 µm, in the group with use fL-norvaline – 91.86±1.8 µm (p<0.001), in the group with use of L-arginine – 86.64±1.39 µm (p<0.001) and in the group with use of strontium ranelate – 89.08±1.09 µm.

Conclusion. L-arginine and L-norvaline and also rosuvastatin possess the property of improving a morphofunctional condition of bone tissue and may be recommended for further preclinical study.

About the authors

Oleg S. Gudyrev

Belgorod State National Research University

Author for correspondence.
Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0003-0097-000X
SPIN-code: 5936-4774
ResearcherId: N-5527-2016

MD, PhD, Associate Professor, Associate Professor of the Department of Pharmacology and Clinical Pharmacology

Russian Federation, Belgorod

Aleksandr V. Faitelson

Kursk State Medical University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0003-3759-6373
SPIN-code: 7389-8181
ResearcherId: A-9809-2017

MD, PhD, Associate Professor, Professor of the Department of Traumatology and Orthopedics

Russian Federation, Kursk

Mikhail S. Sobolev

Kursk State Medical University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0001-7839-2049
SPIN-code: 2987-3470
ResearcherId: A-9473-2019

PhD-Student of the Department of Traumatology and Orthopedics

Russian Federation, Kursk

Mikhail V. Pokrovskiy

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0002-1493-3376
SPIN-code: 9201-3580
ResearcherId: A-4427-2017

MD, PhD, Professor, Head of the Department of Pharmacology and Clinical Pharmacology

Russian Federation, Belgorod

Tat`yana G. Pokrovskaya

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0001-6802-5368
SPIN-code: 8338-0100
ResearcherId: A-5281-2017

MD, PhD, Professor, Professor of the Department of Pharmacology and Clinical Pharmacology

Russian Federation, Belgorod

Mikhail V. Korokin

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0001-5402-0697
SPIN-code: 8385-3508
ResearcherId: A-6652-2017

MD, PhD, Professor, Professor of the Department of Pharmacology and Clinical Pharmacology

Russian Federation, Belgorod

Elena E. Povetka

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0003-4517-1041
SPIN-code: 3609-4411
ResearcherId: A-8035-2019

Student

Russian Federation, Belgorod

Eduard S. Miller

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0001-7982-7518
SPIN-code: 8028-5617
ResearcherId: A-7578-2019

Student

Russian Federation, Belgorod

Vladislav O. Soldatov

Belgorod State National Research University

Email: gudyrev@bsu.edu.ru
ORCID iD: 0000-0001-9706-0699
SPIN-code: 8763-8217
ResearcherId: A-7292-2019

Assistant of the Department of Pharmacology and Clinical Pharmacology

Russian Federation, Belgorod

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2. Fig. 1. Results of the influence of L-arginine, L-norvaline, rosuvastatin and strontium renelate on the blood supply of the proximal metaphysis of femoral bone in 8 weeks after bilateral ovariectomy

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3. Fig. 2. Results of influence of L-arginine, L-norvaline, rosuvastatin and strontium renelate on the average width of trabeculae of proximal metaphysis of femoral bone in 8 weeks after bilateral ovariectomy

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Copyright (c) 2019 Gudyrev O.S., Faitelson A.V., Sobolev M.S., Pokrovskiy M.V., Pokrovskaya T.G., Korokin M.V., Povetka E.E., Miller E.S., Soldatov V.O.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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