Email this article Succinate and succinate dehydrogenase of mononuclear blood leukocytes as markers of adaptation of mitochondria to hypoxia in patients with exacerbation of chronic obstructive pulmonary disease
- Authors: Belskikh E.S.1, Uryasiev O.M.1, Zvyagina V.I.1, Faletrova S.V.1
-
Affiliations:
- Ryazan State Medical University
- Issue: Vol 28, No 1 (2020)
- Pages: 13-20
- Section: Original study
- URL: https://journals.rcsi.science/pavlovj/article/view/14376
- DOI: https://doi.org/10.23888/PAVLOVJ202028113-20
- ID: 14376
Cite item
Abstract
Aim. To study the concentration of succinate and the activity of succinate dehydrogenase (SDH) of mononuclear blood leukocytes as markers of rapid adaptation of mitochondria to hypoxia in patients with exacerbation of chronic obstructive pulmonary disease (COPD).
Materials and Methods. The study involved 58 patients with COPD and 13 conventionally healthy volunteers of 40-75 years of age. In accordance with GOLD 2018 principles of complex assessment, the patients were divided to groups B (n=18), C (n=20), D (n=20) comparable in age, FEV1 and in pack-of-cigarettes/year index. Patients of D group were characterized by more pronounced hypoxemia. Activity of SDH and concentration of succinate were determined in mononuclear leukocytes isolated from blood.
Results. Patients with exacerbation of COPD divided to groups on the basis of the frequency of exacerbations and evidence of symptoms, were characterized by different severity of disorders of mitochondrial functions of mononuclear leukocytes. Patients of C group had the highest succinate concentration (428 [357;545] nmol/106 cells in I ml of suspension) and SDH activity (64[56;73] nmol of succinate/min * 106 cells of 1 ml of suspension) in mononuclear leukocytes as compared to groups B (1.43-times reduction of succinate, p<0.002; 1.88-times reduction of SDH, p=0.0015) and D (2.06-times reduction of succinate, p<0.0001; 4.26-times reduction of SDH, p<0.0001). Patients of D group demonstrated the most pronounced reduction of markers of adaptation to hypoxia.
Conclusions. A small amount of symptoms in exacerbation of COPD is associated with the highest parameters of the mechanism of rapid adaptation of mitochondria of mononuclear leukocytes to hypoxia. Existence of evident symptoms and frequent exacerbations in patents is associated with a severe frustration of mechanisms of adaptation of mitochondria to hypoxia.
Keywords
Full Text
##article.viewOnOriginalSite##About the authors
Eduard S. Belskikh
Ryazan State Medical University
Author for correspondence.
Email: ed.bels@yandex.ru
ORCID iD: 0000-0003-1803-0542
SPIN-code: 9350-9360
Scopus Author ID: 57195313786
ResearcherId: A-7202-2019
PhD-Student of the Department of Faculty Therapy with the Course of Therapy of the Faculty of Additional Postgraduate Education
Russian Federation, RyazanOleg M. Uryasiev
Ryazan State Medical University
Email: ed.bels@yandex.ru
ORCID iD: 0000-0001-8693-4696
SPIN-code: 7903-4609
ResearcherId: S-6270-2016
MD, PhD, Prof., Head of the Department of Faculty Therapy with the Course of Therapy of the Faculty of Additional Postgraduate Education
Russian Federation, RyazanValentina I. Zvyagina
Ryazan State Medical University
Email: ed.bels@yandex.ru
ORCID iD: 0000-0003-2800-5789
SPIN-code: 7553-8641
PhD in Biological Science, Associate Professor of the Department of Biological Chemistry with the Clinical Laboratory Diagnostics of Diseases Course of the Faculty of Additional Postgraduate Education
Russian Federation, RyazanSvetlana V. Faletrova
Ryazan State Medical University
Email: ed.bels@yandex.ru
ORCID iD: 0000-0003-1532-0827
SPIN-code: 1427-8316
Assistant of the Department of Faculty Therapy with the Course of Therapy of the Faculty of Additional Postgraduate Education
Russian Federation, RyazanReferences
- Barabanova EN. GOLD 2017: what change were made in global strategy of treatment of chronic obstructive pulmo-nary disease and why? Pulʹmono-logiâ. 2017;27(2):274-82. (In Russ). doi:10.18093/ 0869-0189-2017-27-2-274-282
- Nizov AA, Ermachkova AN, Abrosimov VN, et al. Complex assessment of the degree of chronic obstructive pulmo-nary disease COPD severity on out-patient visit. I.P. Pavlov Russian Medical Biological Herald. 2019;27(1):59-65. (In Russ). doi:10.23888/ PAVLOVJ201927159-65
- Nam HS, Izumchenko E, Dasgupta S, et al. Mitochondria in chronic obstructive pulmonary disease and lung cancer: where are we now? Biomarkers in Medicine. 2017;11(6):475-89. doi: 10.2217/bmm-2016-0373
- Agrawal A, Mabalirajan U. Rejuvenating cellular respiration for optimizing respiratory function: targeting mitochon-dria. American Journal of Physiology. Lung Cellular and Molecular Physiology. 2016; 310(2):103-13. doi: 10.1152/ajplung.00320.2015
- Lerner CA, Sundar IK, Rahman I. Mitochondrial redox system, dynamics, and dysfunction in lung inflammaging and COPD. International Journal of Biochemistry & Cell Biology. 2016;81(Pt B):294-306. doi: 10.1016/j.biocel.2016.07.026
- Li LA, Lebed'ko OA, Kozlov VK. Assessment of mitochondrial dysfunction in children with community-acquired pneumonia. Far East Medical Journal. 2015;(2):30-6. (In Russ).
- Singh S, Verma SK, Kumar S, et al. Evaluation of Oxidative Stress and Antioxidant Status in Chronic Obstructive Pulmonary Disease. Scandinavian Jour-nal of Immunology. 2017;85(2):130-7. doi:10.1111/ sji.12498
- Lobanova EG, Kondratiyeva EV, Mineyeva EE, et al. The membrane potential of mitochondria of thrombocytes in patients with chronic obstructive disease of lungs. Russian Clinical Laboratory Diagnostics. 2014;59(6):13-6. (In Russ).
- Denisenko YuK, Novgorodtseva TP, Vitkina TI, et al. Mitochondrial dysfunction in chronic obstructive pulmonary disease. Byulleten’ Fiziologii i Patologii Dykhaniya. 2016;(60):28-33. (In Russ). doi: 10.12737/20048
- Lukyanova LD, Kirova YI. Mitochondria-control-led signaling mechanisms of brain protection in hypoxia. Frontiers in Neuroscience. 2015;9:320. doi: 10.3389/fnins.2015.00320
- Belskikh ES, Uryas'ev OM, Zvyagina VI, et al. Investigation of oxidative stress and function of mitochondria in mononuclear leukocytes of blood in patients with chronic bronchitis and with chronic obstructive pulmonary disease. Nauka Molodyh (Eruditio Juvenium). 2018;6(2):203-10. (In Russ). doi: 10.23888/HMJ201862203-210
- Metody biokhimicheskikh issledovaniy: (Lipidnyy i ehnergeticheskiy obmen). Leningrad: Izdatel’stvo Leningrad-skogo gosudarstvennogo universiteta; 1982. (In Russ).
Supplementary files
