Features of neuroimmune and genetic profile in men with clinical manifestations of vegetative vascular dystonia of hypertensive type
- Authors: Dolgikh O.V.1, Zaitseva N.V.1, Nikonoshina N.A.1, Alekseev V.B.1
-
Affiliations:
- Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of Federal Service or Surveillance on Consumer Rights Protection and Human Wellbeing
- Issue: Vol 104, No 6 (2023)
- Pages: 813-821
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/257261
- DOI: https://doi.org/10.17816/KMJ321738
- ID: 257261
Cite item
Abstract
Background. Identification of immune, neurohumoral and genetic markers of vegetative vascular dystonia of the hypertensive type is relevant in the aspect of prevention and early diagnosis of cardiovascular pathology in middle-aged men.
Aim. To study the features of the immune and neurohumoral profile in middle-aged men with clinical manifestations of vegetative vascular dystonia of the hypertensive type, associated with polymorphic variants of candidate genes for blood vessel tone regulation.
Material and methods. 113 middle-aged men (mean age 46.1±3.9 years) were examined. The observation group included 65 men (average age 45.3±4.6 years) with clinical manifestations of vegetative vascular dystonia of the hypertensive type. The comparison group included 48 men without signs of autonomic regulation dysfunction (average age 48.3±3.2 years). The groups were comparable in age (p=0.593), ethnic and social composition. The content of CD3+CD95+ lymphocytes was determined by flow cytometry; interleukin-6, total immunoglobulin E, adrenaline, serotonin — by enzyme immunoassay. Polymorphic variants of the genes ADRB2 (rs1042713), ADD1 (rs4961), GNB3 (rs5443), HTR2A (rs7997012) were identified by real-time polymerase chain reaction. Statistical processing: comparison of arithmetic means using Student's t-test or Mann–Whitney U-test, calculation of odds ratio (OR) and 95% confidence interval (CI).
Results. The immune profile of men with disorders of autonomic regulation was characterized by a deficiency of CD3+CD95+ lymphocytes against the background of overproduction of total immunoglobulin E and interleukin-6. The neurohumoral profile was characterized by excessive synthesis of adrenaline and serotonin. The development of symptoms of vegetative vascular dystonia of the hypertensive type was associated with the G allele of the ADRB2 gene (rs1042713) (OR=1.80; 95% CI=1.05–3.08; p <0.05), the G allele of the ADD1 gene (rs4961) (OR=3.51; 95% CI=1.27–9.67; p <0.05), C allele of the GNB3 gene (rs5443) (OR=2.30; 95% CI=1.21–4.37; p <0.05), A allele of the HTR2A gene (rs7997012) (OR=2.38; 95% CI=1.37–4.15; p <0.05).
Conclusion. Changes in the immune profile were identified (deficiency of CD3+CD95+ lymphocytes, excess of total immunoglobulin E, interleukin-6), associated with hyperproduction of adrenaline and serotonin against the background of polymorphism of candidate genes for vascular tone regulation [ADRB2 (rs1042713), ADD1 (rs4961), HTR2A (rs7997012 ), GNB3 (rs5443)].
Full Text
##article.viewOnOriginalSite##About the authors
Oleg V. Dolgikh
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of Federal Service or Surveillance on Consumer Rights Protection and Human Wellbeing
Email: oleg@fcrisk.ru
ORCID iD: 0000-0003-4860-3145
M.D., D. Sci. (Med.), Head of Depart., Depart. of Immunobiological Diagnostics
Russian Federation, Perm, RussiaNina V. Zaitseva
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of Federal Service or Surveillance on Consumer Rights Protection and Human Wellbeing
Email: znv@fcrisk.ru
ORCID iD: 0000-0003-2356-1145
M.D., D. Sci. (Med.), Prof., Academician of the Russian Academy of Sciences, Scientific Manager
Russian Federation, Perm, RussiaNatalya A. Nikonoshina
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of Federal Service or Surveillance on Consumer Rights Protection and Human Wellbeing
Author for correspondence.
Email: nat08.11@yandex.ru
ORCID iD: 0000-0001-7271-9477
Junior Research, Laboratory of Immunology and Allergology
Perm, RussiaVadim B. Alekseev
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies of Federal Service or Surveillance on Consumer Rights Protection and Human Wellbeing
Email: root@fcrisk.ru
ORCID iD: 0000-0001-5850-7232
M.D., D. Sci. (Med.), Prof., Director
Russian Federation, Perm, RussiaReferences
- Malyutina NN, Paramonova SV, Sedinina NS, Ustinova OYu. Risk of developing hypertension in underground workers when psycho-vegetative status is transforming. Health Risk Analysis. 2021;(4):100–108. (In Russ.) doi: 10.21668/health.risk/2021.4.11.
- Kukhtevich II, Aleshina NI. Vegetative dystonia syndrome as a somatoform disorder. Saratov Journal of Medical Scientific Research. 2019;15(1):159–162. (In Russ.)
- Dolgikh OV, Zaitseva NV, Nikonoshina NA, Alekseev VB. Particular immune and neurohumoral profile indicators in women of fertile age with functional disorders of the autonomic nervous system associated with polymorphic variants of HTR2A (rs7997012) and TP53 (rs1042522) genes. Bulletin of experimental biology and medicine. 2022;172(2):199–204. (In Russ.) doi: 10.47056/0365-9615-2022-173-2-199-204.
- Dyukova GM. Vegetative dystonia syndrome: current view on diagnostics and therapy. Effektivnaya farmakoterapiya. 2017; (31):62–68. (In Russ.)
- Shvarts YuG, Elkina AYu, Akimova NS, Martynovich TV, Fedotov EA. The genes associated with lipid metabolism disorders and with blood pressure regulation, and psychovegetative changes in the young almost healthy persons. Cardiovascular Therapy and Prevention. 2016;15(6):53–57. (In Russ.) doi: 10.15829/1728-8800-2016-6-53-57.
- Klinicheskoe rukovodstvo Titsa po laboratornym testam. (Tietz clinical guide to laboratory tests.) Alan AHB Woo, editor. Translated from English. VV Menshikov, editor. M.: Labora; 2013. 1280 р. (In Russ.)
- Humphrey JD. Mechanisms of vascular remodeling in hypertension. Am J Hypertens. 2021;34(5):432–441. doi: 10.1093/ajh/hpaa195.
- Jia Z, Wang S, Yan H, Cao Y, Zhang X, Wang L, Zhang Z, Lin S, Wang X, Mao J. Pulmonary vascular remodeling in pulmonary hypertension. J Pers Med. 2023;13:366. doi: 10.3390/jpm13020366.
- Ge W, Guo X, Song X, Pang J, Zou X, Liu Y, Niu Y, Li Z, Zhao H, Gao R, Wang J. The role of immunoglobulin E and mast cells in hypertension. Cardiovasc Res. 2022;118(14):2985–2999. doi: 10.1093/cvr/cvac010.
- Rizzoni D, de Ciuceis C, Szczepaniak P, Paradis P, Schiffrin EL, Guzik TJ. Immune system and microvascular remodeling in humans. Hypertension. 2022;79:691–705. doi: 10.1161/HYPERTENSIONAHA.121.17955.
- Didion SP. Cellular and oxidative mechanisms associated with interleukin-6 signaling in the vasculature. Int J Mol Sci. 2017;18(12):2563. doi: 10.3390/ijms18122563.
- Byrne CJ, Khurana S, Kumar A, Tai TC. Inflammatory signaling in hypertension: regulation of adrenal catecholamine biosynthesis. Front Endocrinol (Lausanne). 2018;9:343. doi: 10.3389/fendo.2018.00343.
- Adameova A, Abdellatif Y, Dhalla NS. Role of the excessive amounts of circulating catecholamines and glucocorticoids in stress-induced heart disease. Can J Physiol Pharmacol. 2009;87(7):493–514. doi: 10.1139/y09-042.
- Han D, Choi JH, Kim S, Park SM, Shin DG, Kang M-K, Choi S, Lee N, Cho JR. Changes in serum serotonin levels in patients with acute coronary syndrome and stable angina undergoing percutaneous coronary intervention. J Int Med Res. 2020;48(12):300060520970104. doi: 10.1177/0300060520970104.
- Kiang KM-Y, Zhang P, Li N, Zhu Z, Jin L, Leung GK-K. Loss of cytoskeleton protein ADD3 promotes tumor growth and angiogenesis in glioblastoma multiforme. Cancer Lett. 2020;474:118–126. doi: 10.1016/j.canlet.2020.01.007.
- Ozdemir AC, Wynn GM, Vester A, Weitzmann MN, Neigh GN, Srinivasan S, Rudd MK. GNB3 overexpression causes obesity and metabolic syndrome. PLoS One. 2017;12(12):e0188763. doi: 10.1371/journal.pone.0188763.