DNA-cytometric characteristics of recurrent soft tissue sarcomas

E M Nepomnyashchaya; Непомнящая Евгения Марковна; E P Ul'yanova; Ульянова Елена Петровна; I A Novikova; Новикова Инна Арнольдовна; O N Selyutina; Селютина Олеся Николаевна; E Yu Zlatnik; Златник Елена Юрьевна; T A Aliev; Алиев Тимур Арсенович; L N Vashchenko; Ващенко Лариса Николаевна; T V Ausheva; Аушева Татьяна Валерьевна; E A Andreyko; Андрейко Елена Анатольевна; E I Zolotareva; Золотарёва Екатерина Игоревна; E S Bondarenko; Бондаренко Елена Сергеевна

doi:10.17816/KMJ2018-415

DNA-cytometric characteristics of recurrent soft tissue sarcomas

Authors: Nepomnyashchaya EM¹, Ul'yanova EP¹, Novikova IA¹, Selyutina ON¹, Zlatnik EY.¹, Aliev TA¹, Vashchenko LN¹, Ausheva TV¹, Andreyko EA¹, Zolotareva EI¹, Bondarenko ES¹
Affiliations:
1. Rostov Scientific Research Institute of Oncology
Issue: Vol 99, No 3 (2018)
Pages: 415-420
Section: Theoretical and clinical medicine
URL: https://journals.rcsi.science/kazanmedj/article/view/8893
DOI: https://doi.org/10.17816/KMJ2018-415
ID: 8893

Cite item

Full Text

Abstract

Aim. To determine the content of deoxyribonucleic acid (DNA) and distribution of cells in mitotic phases in patients with recurrent soft tissue sarcomas for the assessment of malignancy of the process.

Methods. Tumor tissues of patients with recurrent soft tissue sarcomas were studied. Research methods included histological, DNA-cytometric and statistical methods.

Results. Proliferative activity and proliferative index of recurrent sarcomas differed depending on the tumor grade and stage. Differences in the number of diploid, aneuploid and polyploid cells were determined in each group and between the groups depending on the cell cycle phases. Cell cycle parameters were as following: 100% of G1 (well-differentiated) cancer were diploid, as well as 33.3% of G2 (moderately differentiated) and 15% of G3 (poorly differentiated) tumors. Aneuploid tumors prevailed in G2 and G3, the ratio of which was 66.7 and 85%, respectively. The analysis of kinetic parameters of the cell cycle allowed establishing a decrease in the number of cells in G1/G0 phase of the cell cycle from G1 to G3, which was accompanied by a statistically significant increase in the proportion of cells in S-phase (p ˂0.05).

Conclusion. The DNA-cytometric study of cell cycle parameters showed high biological potential of recurrent soft tissue sarcomas, which was determined by two indices - the proportion of cells in G2+M-phase and the cell loss factor; 100% of well-differentiated (G1) tumors, 33.3% of moderately differentiated (G2) and 15% of poorly differentiated (G3) tumors were diploid; aneuploid tumors prevailed in G2 and G3.

Keywords

recurrent soft tissue sarcomas, aneuploid cells, diploid cells, proliferative activity

Full Text

##article.viewOnOriginalSite##

References

Siegel R.L., Miller K.D., Jemal A. Cancer statistics. CA Cancer J. Clin. 2016; 66 (1): 7–30. doi: 10.3322/caac.21332.
Zinov'ev G.V., Gafton G.I., Bus'ko E.A. et al. Locally recurrent soft tissue sarcomas of extremities: detection and treatment. Sarkomy kostey, myagkikh tkaney i opukholi kozhi. 2017; (1): 32–41. (In Russ.)
Zinov'ev G.V., Gafton G.I., Bus'ko E.A. et al. Detection of recurrencies soft tissue sarcomas of the extremities. Sibirskiy onkologicheskiy zhurnal. 2017; 16 (2): 82–89. (In Russ.)
Aliev M.D., Men' T.Kh. The incidence of soft tissue sarcomas in Russia. Sarkomy kostey, myagkikh tkaney i opukholi kozhi. 2013; (3): 3–9. (In Russ.)
Stojadinovic A., Leung D.H., Hoos A. et al. Analysis of the prognostic significance of microscopic margins in 2,084 localized primary adult soft tissue sarcomas. Ann. Surg. 2002; 235: 424–434. doi: 10.1097/00000658-200203000-00015.
Bazanov K.V. Research of growth rate of recurrent and metastatic sarcomas of soft tissues. Sovremennye problemy nauki i obrazovaniya. 2014; (6): 1227. (In Russ.)
Kolobov A.V., Polezhaev A.A. Influence of random malignant cell motility on growing tumor front stability. Komp'yuternye issledovaniya i modelirovanie. 2009; 1 (2): 225–232. (In Russ.)
Nerodo G.A., Novikova I.A., Nikitina V.P. et al. Clinical value of DNA flow cytometry in ovarian cancer. Sovremennye problemy nauki i obrazovaniya. 2016; (3): 35. (In Russ.)
Tevryukova N.S., Bogatyrev V.N. Significance of DNA-flow laser cytometry in a differential cytological diagnosis and prostate cancer prognosis. Rossiyskiy onkologicheskiy zhurnal. 2013; (1): 10–19. (In Russ.)
Abdalla F., Buhmeida A., Alshrad M. et al. Prognostic significance of DNA image cytometry in libyan breast cancer. Oncology. 2012; 83: 165–176. doi: 10.1159/000339788.
Gazic B., Pizem J., Bracko M. et al. S-phase fraction determined on fine needle aspirates is an independent prognostic factor in breast cancer — a multivariate study of 770 patients. Cytopathology. 2008; 19 (5): 294–302. doi: 10.1111/j.1365-2303.2007.00528.x.
Kravets O.A., Bogatyrev V.N. The value of laser flow DNA-cytofluorometry in radiation therapy of cervical cancer. Effektivnaya farmakoterapiya. 2012; (2): 42–47. (In Russ.)
Susini T., Olivieri S., Molino C. et al. DNA ploidy is stronger than lymph node metastasis as prognostic factor in cervical carcinoma: 10-year results of a prospective study. Int. J. Gynecol. Cancer. 2011; 21 (4): 678–684. doi: 10.1097/IGC.0b013e3182126f85.
Novikova I.A., Nepomnyashchaya E.M., Ul'yanova E.P. et al. DNA-cytometric characteristics of primary soft tissue sarcomas. Kazanskiy meditsinskiy zhurnal. 2017; 98 (4): 509–513. (In Russ.)
Gut R., Zharinov G.M., Yakubov E. A simplified model for kinetics of a tumor cells' population. Voprosy onkologii. 2009; 55 (1): 83–88. (In Russ.)

Supplementary files

Supplementary Files

Action

1. JATS XML

Download

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

DNA-cytometric characteristics of recurrent soft tissue sarcomas

Full Text

Abstract

Keywords

Full Text

About the authors

References

Supplementary files