Antiimmunosuppressive action of 3d-metal gluconates in experimental immunodeficiency
- Authors: Knyazeva OA1, Urazaeva SI1, Konkina IG2, Saptarova LM1, Gazdalieva LM1, Murinov Y.I2
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Affiliations:
- Bashkir State Medical University
- Ufa Institute of Chemistry of the Russian Academy of Sciences
- Issue: Vol 99, No 2 (2018)
- Pages: 255-259
- Section: Experimental medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/8416
- DOI: https://doi.org/10.17816/KMJ2018-255
- ID: 8416
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Abstract
Aim. Evaluation of the effect of 3d-metal gluconates on complement-fixing function of immunoglobulin G and functional activity of complement.
Methods. The study was conducted in vivo on 2.5-3 month-old white laboratory mice weighing 25-28 g with secondary immunodeficiency, which was induced by a single intraperitoneal injection of cyclophosphamide, as well as in vitro in a test system using sensitized sheep erythrocytes. Immunological studies were performed in intact animals, and before and after the administration of Mn, Co, Cu, and Zn gluconates to mice with induced immunodeficiency. The content of immunoglobulin G and its complexes with subcomponent of the complement first component C1q was determined in serum by ELISA using specific monoclonal antibodies.
Results. Two-week oral administration of 3d-metal gluconates (Mn, Co, Cu, Zn) in a dose of 1/10 LD50 to immunodeficient mice was shown to cause a significant increase in the level of immunoglobulin G and its complexes with C1q. The greatest increase in concentration was observed with the introduction of zinc gluconate. Also by means of sensitized sheep erythrocytes in vitro, cobalt and, to a lesser extent, manganese gluconates were shown to increase the functional activity of C1q.
Conclusion. 3d-metal gluconates (Mn, Co, Cu, Zn) demonstrate immunocorrecting properties: increase the content of immunoglobulin G and its complexes with C1q, significantly decreasing as a result of cyclophosphamide effect; cobalt and manganese gluconates have a stimulating effect on the functional activity of complement by its classical pathway, which indicates different mechanisms of immunocorrection action of studied metal gluconates and requires further studies.
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##article.viewOnOriginalSite##About the authors
O A Knyazeva
Bashkir State Medical University
Author for correspondence.
Email: olga_knyazeva@list.ru
Ufa, Russia
S I Urazaeva
Bashkir State Medical University
Email: olga_knyazeva@list.ru
Ufa, Russia
I G Konkina
Ufa Institute of Chemistry of the Russian Academy of Sciences
Email: olga_knyazeva@list.ru
Ufa, Russia
L M Saptarova
Bashkir State Medical University
Email: olga_knyazeva@list.ru
Ufa, Russia
L M Gazdalieva
Bashkir State Medical University
Email: olga_knyazeva@list.ru
Ufa, Russia
Yu I Murinov
Ufa Institute of Chemistry of the Russian Academy of Sciences
Email: olga_knyazeva@list.ru
Ufa, Russia
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