To the method of cultivation of spiro ch. pallida

Due to the need to study the immunobiological properties of sp. pallida, the preparation of antigens from these spirochetes for practical purposes, we faced the question of obtaining mass cultures of sp. pallida. To obtain such k-p, on the one hand, the cultivation technique should be simplified as much as possible, and on the other hand, the nutrient media used should not contain pieces of organs that easily disintegrate and turn into sediment during centrifugation. The release of cultures from these particles by settling and merging is associated with the loss of a large amount of material. In this regard, it would be of great importance to grow sp. pallida either with pieces of such organs that would not give turbidity to the medium, or growing without adding pieces of animal tissue. As for the first possibility, despite the addition of pieces of such relatively difficult-to-loosen organs as the heart or lung to the nutrient broth, the medium still becomes cloudy, and thus, during centrifugation, the smallest particles of these organs accumulate in the sediment. Regarding the development of spirochetes, it must be said that on such media consisting of a single horsemeat broth, pH = 7.6, with the addition of pieces of heart or lung muscle, they develop well, but all the development comes somewhat slower and the number of spirochetes is less than on the medium we usually use with pieces of bull liver.