Morphological characteristics of experimental ischemic wound healing after application of autoand hetero-fibroblasts and dermal equivalent
- Authors: Shapovalova EY.1, Boyko TA1, Baranovskiy Y.G1, Lugin IA1
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Affiliations:
- Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University
- Issue: Vol 99, No 1 (2018)
- Pages: 53-59
- Section: Experimental medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/7809
- DOI: https://doi.org/10.17816/KMJ2018-053
- ID: 7809
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Abstract
Aim. To study morphological structure, collagen synthesis and angiogenesis in biopsy samples of newly formed epidermis and derm on day 19 of their repair in experimental ischemic wound after application of auto- and hetero-fibroblasts as well as after transplantation of dermal equivalent with hetero-fibroblasts.
Methods. The study was performed on 28 mature C57/B1 white mice younger than 1 year. Around and into the bed of surgical skin wound in the scapular region, 0.4 ml of suspension of fibroblasts and 1.33 million cells of dermal equivalent were injected. Biopsy sample was embedded in paraffin and was stained with hematoxylin and eosin and by Weigert-Van Gieson.
Results. Most importantly, regenerative histogenesis is affected by introduction of auto-fibroblasts, when the greatest thickness of the epidermis is registered and angiogenesis and collagen formation are most active. At the same time, the most differentiated epidermis is observed after transplantation into the wound of dermal equivalent with hetero-bifroblasts.
Conclusion. The beneficial effect of dermal equivalent with hetero-bifroblasts differs from the effect of auto-fibroblast suspension by only a few percent: epidermal thickness by 4.29%, the area of collagen fibers by 2.66%, the area of blood vessels by 4.04%, that makes such differences unreliable.
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##article.viewOnOriginalSite##About the authors
E Yu Shapovalova
Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University
Author for correspondence.
Email: Shapovalova_L@mail.ru
Simferopol, Russia
T A Boyko
Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University
Email: Shapovalova_L@mail.ru
Simferopol, Russia
Yu G Baranovskiy
Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University
Email: Shapovalova_L@mail.ru
Simferopol, Russia
I A Lugin
Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University
Email: Shapovalova_L@mail.ru
Simferopol, Russia
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