Clinical and laboratory assessment of the application efficacy of antibacterial combinations in the treatment of chronic generalized periodontitis
- Authors: Puri-Zahidan SV1
-
Affiliations:
- Azerbaijan Medical University
- Issue: Vol 101, No 3 (2020)
- Pages: 356-364
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/34617
- DOI: https://doi.org/10.17816/KMJ2020-356
- ID: 34617
Cite item
Abstract
Aim. To determine the efficacy of the combination of metronidazole and co-trimoxazole in the treatment of chronic generalized periodontitis.
Methods. The studies included patients with slight and moderate chronic generalized periodontitis without concomitant pathology. Patients were divided into two groups with similar age and severity of periodontal diseases. In the first group, metronidazole was administered into the periodontal pocket; in the second group co-trimoxazole and metronidazole combination was used. Oral fluid testing was used to detect ureolytic and glycolytic activity, malondialdehyde (MDA) content, conjugated dienes, catalase activity, Salivary Secretory Immunoglobulin (SIgA) and Lysozyme. Changes in periodontal disease were assessed before and after treatment. The duration of treatment was 12 days.
Results. Co-trimoxazole and metronidazole combination more effectively stimulated ureolytic activity and reduced glycolytic effect. It had a more effective antioxidant effect that manifested in: a statistically significant decrease in malondialdehyde levels by 32.8% (p <0.01) and by 36.6% (p <0.01) in patients with slight and moderate periodontitis, respectively; decrease in diene conjugates by 25.0% (p <0.001) and 37.7% (p <0.001); and increased catalase activity 2.6 and 2.9 times (p <0.001). After treatment, it was revealed an increase in lysozyme activity by 11.3% and 17.6% (р <0,001) in patients with slight and moderate periodontitis, respectively, and increase secretory IgA levels by 37.4% (p <0.001) and 53.2% (p <0.001). In the first group, the amount of SIgA in the oral fluid increases by 35.8% (p <0.001), and by 45.6% (p <0.001) compared to the value before treatment in patients with slight and moderate periodontitis, and in the second group by 37.4% (p <0.001) and 53.2% (p <0.001), respectively.
Conclusion. The combined treatment with co-trimoxazole + metronidazole leads to the positive changes in the studied parameters causes rapid disappearance of the symptoms characteristic of chronic generalized periodontitis; this 2-drug combination is recommended to be included in the complex therapeutic measures for inflammatory periodontal diseases.
Full Text
##article.viewOnOriginalSite##About the authors
S V Puri-Zahidan
Azerbaijan Medical University
Author for correspondence.
Email: aga70@bk.ru
Azerbaijan, Baku, Azerbaijan
References
- Blashkova S.L., Makarova N.A. Report of conducting patients with chronic generalizating paradontitis. Prakticheskaya meditsina. 2009; (1): 63–67. (In Russ.)
- Khaliullina G.R., Blashkova S.L., Makarova N.A. Principles of the pathogenetic therapy of patients with chronic moderate generalized parodontitis. Prakticheskaya meditsina. 2013; (4): 78–80. (In Russ.)
- Bidra A.S., Shaqman M. Treatment planning and sequence for implant therapy in a young adult with generalized aggressive periodontitis. J. Oral. Implantol. 2012; 38 (4): 405–415. doi: 10.1563/AAID-JOI-D-10-00118.
- Zabolevaniya parodonta. (Periodontal diseases.) Ed. by L.Y. Orekhovoj. M. 2004; 201–247. (In Russ.)
- Feres M., Soares G.M., Mendes J.A. et al. Metronidazole alone or with amoxicillin as adjuncts to non-surgical treatment of chronic periodontitis: a 1-year double-blinded, placebo-controlled, randomized clinical trial. J. Clin. Periodontol. 2012; 39: 1149–1158. doi: 10.1111/jcpe.12004.
- Gorkunova A.R. Change in biochemical parameters in the oral fluid with secondary adentia on the background of chronic generalized periodontitis. Nauchnoe obozrenie. Meditsinskie nauki. 2015; (1): 136–137. (In Russ.)
- Andreeva L.I., Kozhemyakin L.A., Kishkun A.A. Modification of the method for determining lipid peroxides in the test with thiobarbituric acid. Laboratornoe delo. 1988; (11): 41–43. (In Russ.)
- Gavrilov V.B., Gavrilova A.R., Khmara N.F. Change in diene conjugates in blood plasma by UV absorption of heptane and isopropanol extracts. Laboratornoe delo. 1988; (2): 60–64. (In Russ.)
- Goryachkovskiy A.M. Klinicheskaya biokhimiya. (Clinical biochemistry.) Odessa: Astroprint. 1998; 603 р. (In Russ.)
- Dorofeychuk V.G. Determination of lysozyme activity by the nephelometric method. Laboratornoe delo. 1968; (1): 28–30. (In Russ.)
- Mancini G., Corbonard A.O., Heremdus J.F. Immunochemical quantitation of antigen by single radial immunodiffusion. Immunochemistry. 1965; 2 (3): 235–254.
- Khramov V.A., Gavrikova L.M. Determination of ureolytic and glycolytic activity of human oral fluid. Stomatologiya. 1996; (3): 7–9. (In Russ.)
- Firsova I.V., Mikhalchenko D.V., Malyukov A.V. Patient compliance in dental practice. Sotsiologiya meditsinyi. 2009; (1): 20–22. (In Russ.)