Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus
- Authors: Skibo YV1, Fathullina AR1, Ibragimov BR1, Abramov SN1, Ismagilova RR2, Biktagirova EM1, Andrianova IA1, Maksudova AN2, Abramova ZI1
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Affiliations:
- Kazan (Volga Region) Federal University
- Kazan State Medical University
- Issue: Vol 101, No 3 (2020)
- Pages: 347-355
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/25798
- DOI: https://doi.org/10.17816/KMJ2020-347
- ID: 25798
Cite item
Abstract
Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus.
Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann–Whitney test.
Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found.
Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.
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##article.viewOnOriginalSite##About the authors
Y V Skibo
Kazan (Volga Region) Federal University
Author for correspondence.
Email: yuliya_ksu@mail.ru
SPIN-code: 3727-5511
Russian Federation, Kazan, Russia
A R Fathullina
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
SPIN-code: 8511-6349
Russian Federation, Kazan, Russia
B R Ibragimov
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
S N Abramov
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
ORCID iD: 0000-0003-3174-4363
SPIN-code: 1489-1208
Scopus Author ID: 56595507300
ResearcherId: A-2414-2019
Russian Federation, Kazan, Russia
R R Ismagilova
Kazan State Medical University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
E M Biktagirova
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
I A Andrianova
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
A N Maksudova
Kazan State Medical University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
Z I Abramova
Kazan (Volga Region) Federal University
Email: yuliya_ksu@mail.ru
Russian Federation, Kazan, Russia
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