The role of interfering RNA in immune response proliferative processes regulation in experimental model of endometrial cancer associated with thyroid disease

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Abstract

Aim. To assess the proliferation and dendritic cells markers expression degree at short interference RNA (siRNA) transfection in endometrial cancer associated with experimental thyroid disease. Methods. Experiments were performed on female rats distributed to five groups: I - control group; IIA and IIIА - animals with simulated hypo-and hyperthyroidism and transplanted Guérin’s carcinoma, IIВ and IIIB - animals with simulated hypo- and hyperthyroidism and transplanted Guérin’s carcinoma in combination with siRNA transfection. Orthogonal tumor dimensions were measured starting from the 7-th day after tumor suspension inoculation. Proliferation and dendritic cells markers expression were assessed in tumor samples by immunohistochemistry after the exclusion of animals from the experiment. Results. siRNA inhibitory effect was more marked in animals with hypothyroidism, indicating an important role of thyroid hormones in regulating cell cycle controlling genes expression. Transfection of siRNA increased mature dendritic cells (CD83) expression in tumor tissue in animals with hypothyroidism and increased immature dendritic cells (CD1a) expression in tumor tissue in animals with hyperthyroidism. Conclusion. siRNA transfection inhibits the tumor cells proliferation mainly at hypothyroidism compared to hyperthyroidism.

About the authors

V M Zaporozhan

Odessa National Medical University, Odessa, Ukraine

G S Maryniuk

Odessa National Medical University, Odessa, Ukraine

O L Kholodkova

Odessa National Medical University, Odessa, Ukraine

Email: kholodkova_l@rambler.ru

D U Andronov

Odessa National Medical University, Odessa, Ukraine

References

  1. Баударбекова М.М. Експресiя Кi-67 та PCNA при гiперпластичних станах та аденокарциномi ендометрiя у жiнок в перименопаузальному станi // Онкологiя. - 2010. - Т. 12, №4. - С. 394.
  2. Боровиков В.П. STATISTICA: искусство анализа данных на компьютере (2-е издание). - М.: Медицина, 2007. - 700 с.
  3. Зак М.Ю. Клiтинне оновлення у слизовiй оболонцi шлунка у хворих на хронiчний атрофiчний гастрит // Сучасна гастроентерол. - 2011. - Т. 2, №58. - С. 27-32.
  4. Beruchashvili M.V., Tsagareli Z.G., Gogiashvili L.E. et al. Features of immunohistochemical markers expression in cervical epithelium under thyroid dysfunction // Georgian Med. News. - 2011. - Vol. 195. - С. 32-37.
  5. Brent G.A. Mechanisms of thyroid hormone action // J. Clin. Inves. - 2012. - Vol. 122, N 9. - P. 3035-3043.
  6. Dedecjus M., Stasiolek M., Brzezinski J. et al. Thyroid hormones influence human dendritic cells’ phenotype, function, and subsets distribution // Thyroid. - 2011. - Vol. 21, N 5. - Р. 533-540.
  7. George A.L., Rajoria S., Suriano R. et al. Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence // Mol. Cancer. - 2012. - Vol. 1. - Р. 80.
  8. Klecha A.J., Genaro A.M., Gorelik G. et al. Integrative study of hypothalamus-pituitary-thyroid-immune system interaction: thyroid hormone-mediated modulation of lymphocyte activity through the protein kinase C signaling pathway // J. Endocrinol. - 2006. - Vol. 189, N 1. - Р. 45-55.
  9. Liqun Z., Cooper-Kuhn C.M., Nannmark U. et al. Stimulatory effects of thyroid hormone on brain angiogenesis in vivo and in vitro // J. Cereb. Blood Flow. Metab. - 2010. - Vol. 30, N 2. - Р. 323-335.
  10. McNaughton B.R., Cronican J.J., Thompson D.B., Liu D.R. Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins // Proc. Natl. Acad. Sci. USA. - 2009. - Vol. 106, N 15. - Р. 6111-6116.
  11. Ni Y.H., Wang Z.Y., Huang X.F. et al. Effect of siRNA-mediated downregulation of VEGF in Tca8113 cells on the activity of monocyte-derived dendritic cells // Oncol. Lett. - 2012. - Vol. 3, N 4. - P. 885-892.
  12. Shibata M.A., Ambati J., Shibata E. et al. Mammary cancer gene therapy targeting lymphangiogenesis: VEGF-C siRNA and soluble VEGF receptor-2, a splicing variant // Med. Mol. Morphol. - 2012. - Vol. 45, N 4. - Р. 179-184.
  13. Stasiołek M., Adamczewski Z., Puła B. et al. Distribution of subpopulations of dendritic cells in peripheral blood of patients treated with exogenous thyrotropin // Thyroid Res. - 2012. - Vol. 5, N 1. - Р. 18.
  14. Wincewicz A., Baltaziak M., Kanczuga-Koda L. et al. STAT3 and apoptosis regulators: Bak and Bcl-xL in endometrioid adenocarcinomas of different estrogenreceptor-α immunoprofile // Gynecol. Endocrinol. - 2011. - Vol. 27, N 8. - Р. 536-540.

© 2013 Zaporozhan V.M., Maryniuk G.S., Kholodkova O.L., Andronov D.U.

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