Functional status of neutrophils at 2,4-dichlorophenoxyacetic acid amine salt intoxication
- Authors: Akhmetchenko ZA1, Mufazalova NA2, Mufazalova LF2, Remezova AD2, Mukhametzyanova AY.2
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Affiliations:
- Bashkir State University, Ufa, Russia
- Bashkir State Medical University, Ufa, Russia
- Issue: Vol 94, No 5 (2013)
- Pages: 649-651
- Section: Actual problems of biochemistry and laboratory diagnostics
- URL: https://journals.rcsi.science/kazanmedj/article/view/1910
- DOI: https://doi.org/10.17816/KMJ1910
- ID: 1910
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Abstract
Aim. To study the influence of 2,4-dichlorophenoxyacetic acid amine salt subacute intoxication on the functional status of neutrophils. Methods. 2,4-dichlorophenoxyacetic acid amine salt (42 mg/kg) was administered intragastrically during 28 days. The number of leukocytes, neutrophils and lymphocytes in peripheral blood, intensity of oxygen-dependent metabolism (spontaneous and induced tetrazolium test), absorbency, microbicidal activity of neutrophils in conditions of oxygen-dependent microbicidal factors functioning and sodium azide mediated blockade, the level of myeloperoxidase and cation proteins in neutrophils were registered at days 7th and 14th after the toxicant administration discontinuation. Results. Subacute 2,4-dichlorophenoxyacetic acid amine salt intoxication resulted in leucopenia due to neutropenia, accompanied by inhibition of oxygen-dependent neutrophils killing as the result of peroxidase-independent microbicidal mechanisms suppression (reduced synthesis of oxygen active forms). A reduced activity of oxygen-independent killing mechanisms correlating with a decrease of cation proteins levels was also registered. On the 14 day, leucopenia was replaced by leukocytosis due to elevated numbers of neutrophils and lymphocytes in the peripheral blood. At the same time, the activity of both oxidative peroxidase-dependent and oxygen-independent microbicidal mechanisms in neutrophils continued to decrease, accompanied by reduction of mieloperoxidase activity, neutrophil absorbency and cation proteins levels. Conclusion. Subacute intoxication with 2,4-dichlorophenoxyacetic acid amine salt leaded to quantitative and functional disorders in neutrophils that persisted by the 14 day of follow-up, indicating a need for further study of of 2,4-dichlorophenoxyacetic acid amine salt immunotoxic action to find the possible opportunities for its pharmacological correction.
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##article.viewOnOriginalSite##About the authors
Z A Akhmetchenko
Bashkir State University, Ufa, Russia
N A Mufazalova
Bashkir State Medical University, Ufa, Russia
L F Mufazalova
Bashkir State Medical University, Ufa, Russia
Email: lya-mufazalova@ya.ru
A D Remezova
Bashkir State Medical University, Ufa, Russia
A Ya Mukhametzyanova
Bashkir State Medical University, Ufa, Russia
References
- Волчегорский И.А., Долгушин И.И., Колесников О.Л. и др. Экспериментальное моделирование и лабораторная оценка адаптивных реакций организма. - Челябинск: Изд-во Челябинского государственного педагогического ун-та, 2000. - 167 с.
- Гареев Е.М. Основы математико-статистической обработки медико-биологической информации. - Уфа: Башгосмедуниверситет Роздрава, 2009. - 346 с.
- Долгушин И.И., Андреева Ю.С., Савочкина А.Ю. Нейтрофильные ловушки и методы оценки функционального статуса нейтрофилов. - М.: Изд-во РАМН, 2009. - 208 с.
- Забродский П.Ф., Мандыч В.Г. Иммунотоксикология ксенобиотиков. - Саратов: Изд-во СВИБХБ, 2007. - 420 с.
- Камилов Ф.Х. Патохимия токсического действия хлорорганических и ароматических соединений // Мед. вестн. Башкортостана. - 2007. - Т. 2, №6. - С. 76-80.
- Комарова Н.В., Карцова А.А. Пестициды группы хлорфеноксикарбоновых кислот. Анализ в водных объектах методом капиллярного электрофореза // Аналит. хим. - 2004. - http://www.novedu.ru/2000/pitvod.htm#pes (дата обращения: 01.08.13).
- Лебедева Н., Жамсаранова Д. Использование пептидных биорегуляторов при отравлении гербицидом 2,4-дихлорфеноксиуксусной кислоты // Гигиена и санитария. - 2004. - №4. - С. 68-70.
- Мартыненко В.И., Прамоненков В.К., Кукаленко С.С. и др. Пестициды (справочник). - М.: Агропромиздат, 1992. - С. 209-212.
- Справочник пестицидов и агрохимикатов, разрешённых к применению на территории Российской Федерации в 2012 году // Защита и карантин растений: прил. к ж. - 2012. - №6. - 850 с.
- Хизбуллин Ф.Ф. Диоксины в жизненном цикле хлорорганических химических продуктов. - Уфа: Реактив, 2005. - 180 с.
- Ягода А.В., Локтев Н.А. Клиническая цитохимия. - Ставрополь: СтГМА, 2005. - 485 с.
- Troudi A., Soudani N., Mahjoubi S.A. et al. 2,4-Dichlorophenoxyacetic acid effects on nephrotoxicity in rats during late pregnancy and early postnatal periods // Ecotoxicol. Environ. Saf. - 2011. - Vol. 74,N 8. - P. 2316-2323.
- Schreinemachers D.M. Perturbation of lipids and glucose metabolism associated with previous 2,4-D exposure: a crosssectional study of NHANES III data, 1988-1994 // Environm. Health. - 2010. - Vol. 9. - P. 11.
- Soloneski S., González N.V., Reigosa M.A. et al. Herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)-induced cytogenetic damage in human lymphocytes in vitro in presence of erythrocytes // Cell Biol. Int. - 2007. - Vol. 31. - P. 1316-1322.
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