Effect of autologous mesenchymal pluripotent stem cells transplantation on liver microcirculation in rats with experimental liver cirrhosis
- Authors: Dzhafarli RE1,2
-
Affiliations:
- Azerbaijan Medical University, Baku, Azerbaijan
- Institute of Urgent and Reconstructive Surgery named after V.K. Gusak, Donetsk, Ukraine
- Issue: Vol 96, No 2 (2015)
- Pages: 198-202
- Section: Experimental medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/1741
- DOI: https://doi.org/10.17750/KMJ2015-198
- ID: 1741
Cite item
Full Text
Abstract
Aim. To study the effect of autologous bone marrow pluripotent stem cells transplantation on liver microcirculation in experimental model of liver cirrhosis.
Methods. 43 white Wistar male rats with body weight of 150-180 g aged at least 3 months were used, in which autologous pluripotent mesenchymal stromal cells transplantation was performed. Considering the animals mortality at the cirrhosis modeling stage, which was 9.3% (4 out of 43 rats), the first group included 19 rats in which stromal cells were transplanted into the portal vein; in the second group (20 rats) the cells were injected into the common hepatic artery. Liver microcirculation was studied using laser Doppler flowmetry and wavelet analysis. Examinations were performed during the operation prior to autologous pluripotent mesenchymal stromal cells transplantation in rats with experimental liver cirrhosis, as well as on the 8th week of treatment.
Results.In modeled liver cirrhosis, the microcirculation index was decreased by 24.5% (p
Conclusion. The repeated studies of microcirculation based on laser Doppler during the treatment of animals with experimental liver cirrhosis indicates the advantages of intra-arterial autologous multipotent mesenchymal stromal cells transplantation.
Keywords
About the authors
R E Dzhafarli
Azerbaijan Medical University, Baku, Azerbaijan; Institute of Urgent and Reconstructive Surgery named after V.K. Gusak, Donetsk, Ukraine
Author for correspondence.
Email: dr-jafarli@mail.ru
References
- Подымова С.Д. Болезни печени. - М.: Медицина, 2005. - 768 с.
- Садовникова И.И. Циррозы печени. Вопросы этиологии, патогенеза, клиники, диагностики, лечения // Рус. мед. ж.: Бол. органов пищеварен. - 2003. - Т. 5, №2. - С. 38-42.
- Хок М.М., Левитан Б.Н., Чалов В.В., Бредихин М.В. Исследование микроциркуляции кожи методом лазерной допплерофлоуметрии при хронических диффузных заболеваниях печени (ХДЗП) // Рос. ж. гастроэнтерол., гепатол., колопроктол. - 2008. - T. 18, №1. - С. 14.
- Jang Y., Kim M., Cho M. et al. Effect of bone marrow-derived mesenchymal stem cells on hepatic fibrosis in a thioacetamide-induced cirrhotic rat model // BMC Gastroenterol. - 2014. - Vol. 14, N 1. - P. 198. http://dx.doi.org/10.1186/s12876-014-0198-6
- Kharaziha P., Hellström P., Noorinayer B., Farzaneh F. Improvement of liver function in liver cirrhosis patients after autologous mesenchymal stem cell injection: a phase I-II clinical trial // Eur. J. Gastroenterol. Hepatology. - 2009. - Vol. 21, N 10. - P. 1199-1205. http://dx.doi.org/10.1097/MEG.0b013e32832a1f6c
- Lee S., Kim K., Park K. Mechanisms of fibrogenesis in liver cirrhosis: the molecular aspects of epithelial-mesenchymal transition // J. Hepatol. - 2014. - Vol. 27, N 4. - Р. 207-216.
- Motawi T., Atta H., Sadik N., Azzam M. The therapeutic effects of bone marrow-derived mesenchymal stem cells and simvastatin in a rat model of liver fibrosis // Cell. Biochem. Biophys. - 2014. - Vol. 68, N 1. - P. 111-125. http://dx.doi.org/10.1007/s12013-013-9698-1
- Wang Y., Lian F., Li J. et al. Adipose derived mesenchymal stem cells transplantation via portal vein improves microcirculation and ameliorates liver fibrosis induced by CCl4 in rats // J. Transl. Med. - 2012. - Vol. 26. - P. 133-141. http://dx.doi.org/10.1186/1479-5876-10-133
- Xu L., Gong Y., Wang B. et al. Randomized trial of autologous bone marrow mesenchymal stem cells transplantation for hepatitis B virus cirrhosis: regulation of Treg/Th17 cells // J. Gastroenterol. Hepatol. - 2014. - Vol. 29, N 8. - P. 1620-1628. http://dx.doi.org/10.1111/jgh.12653
- Yuan S., Jiang T. Use of bone mesenchymal stem cells to treat rats with acute liver failure // Genet. Mol. Res. - 2014. - Vol. 30. - P. 13. http://dx.doi.org/10.4238/2014.April.30.10