Evaluation of the presence and localization of P2 receptors in human blood vessels
- Authors: Ziganshin BA1, Slavin DA2, Khaziakhmetov DF1, Ziganshina AP1, Slavin LE2, Dzhordzhikiya RK1, Ziganshin AU1
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Affiliations:
- Kazan State Medical University, Kazan, Russia
- Kazan State Medical Academy, Kazan, Russia
- Issue: Vol 96, No 3 (2015)
- Pages: 368-376
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/1668
- DOI: https://doi.org/10.17750/KMJ2015-368
- ID: 1668
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Full Text
Abstract
Aim. To study the presence and localization of the P2X and P2Y receptor subtypes in the human cystic artery and great saphenous vein (with and without varicose disease).
Methods. Segments of the human blood vessels were stained using a standard two-step immunohistochemical analysis using primary and secondary antibodies. In the experiments primary antibodies to the following receptors were used: Р2Х1, Р2Х2, Р2Х3, Р2Х4, Р2Y1, Р2Y2, Р2Y4. In order to determine the presence of a receptor in a vessel sample a comparison was made between staining of the experimental and the control samples, which were not treated with primary antibodies.
Results. Immunohistochemical analysis of the cystic artery showed the presence of Р2Х1, Р2Х3, Р2Y1, Р2Y2 receptors. All receptor subtypes were found to be located in the muscular layer of the artery, whereas the P2Y1 receptor was also expressed on the surface of the endothelial cells. In the great saphenous vein without varicose disease Р2Х1, Р2Х2 и Р2Y1 receptor subtypes were identified, all of which were found to be located on the smooth muscle cells of the vein. Similarly to the cystic artery, the Р2Y1 receptor was also found within the endothelial layer of the vein. At the same time, only Р2Х2 и Р2Y1 receptor subtypes were expressed in the muscular layer of the great saphenous vein affected by varicose disease. No P2 receptor subtypes were identified on the endothelial layer of the varicose-diseased vein.
Conclusion. Different P2 receptor subtypes were found to be present in the smooth muscle and endothelial layers of the human cystic artery and great saphenous vein. The identified differences in the receptor subtypes between samples of great saphenous veins with and without varicose disease are, most likely, explained by the restructuring of the receptor apparatus as a result of varicose disease progression.
About the authors
B A Ziganshin
Kazan State Medical University, Kazan, Russia
Author for correspondence.
Email: auziganshin@gmail.com
D A Slavin
Kazan State Medical Academy, Kazan, Russia
Email: auziganshin@gmail.com
D F Khaziakhmetov
Kazan State Medical University, Kazan, Russia
Email: auziganshin@gmail.com
A P Ziganshina
Kazan State Medical University, Kazan, Russia
Email: auziganshin@gmail.com
L E Slavin
Kazan State Medical Academy, Kazan, Russia
Email: auziganshin@gmail.com
R K Dzhordzhikiya
Kazan State Medical University, Kazan, Russia
Email: auziganshin@gmail.com
A U Ziganshin
Kazan State Medical University, Kazan, Russia
Email: auziganshin@gmail.com
References
- Зиганшин А.У., Зиганшин Б.А., Гиниятова Л.Р., Джорджикия Р.К. Влияние PPADS на Р2Х-рецептор-опосредованные ответы кровеносных сосудов человека // Бюлл. эксперим. биол. и мед. - 2004. - Т. 137, №3. - С. 321-324. http://dx.doi.org/10.1023/B:BEBM.0000031571.45054.f0
- Зиганшин Б.А., Славин Д.А., Зиганшина А.П. и др. Функциональная активность варикозно изменённой большой подкожной вены человека на разных её участках // Бюлл. эксперим. биол. и мед. - 2013. - Т. 155, №4. - С. 419-423. http://dx.doi.org/10.1007/s10517-013-2169-2
- Badier-Commander C., Couvelard A., Henin D. et al. Smooth muscle cell modulation and cytokine overproduction in varicose veins. An in situ study // J. Pathol. - 2001. - Vol. 193. - P. 398-407. http://dx.doi.org/10.1002/path.819
- Burnstock G. Noradrenaline and ATP as cotransmitters in sympathetic nerves // Neurochem. Int. - 1990. - Vol. 17. - P. 357-368. http://dx.doi.org/10.1016/0197-0186(90)90158-P
- Burnstock G. Purine and pyrimidine receptors // Cell. Mol. Life Sci. - 2007. - Vol. 64. - P. 1471-1483. http://dx.doi.org/10.1007/s00018-007-6497-0
- Burnstock G. Purinergic mechanisms and pain - an update // Eur. J. Pharmacol. - 2013. - Vol. 716. - P. 24-40. http://dx.doi.org/10.1016/j.ejphar.2013.01.078
- Burnstock G., Ralevic V. Purinergic signaling and blood vessels in health and disease // Pharmacol. Rev. - 2014. - Vol. 66. - P. 102-192. http://dx.doi.org/10.1124/pr.113.008029
- Idzko M., Ferrari D., Eltzschig H.K. Nucleotide signalling during inflammation // Nature. - 2014. - Vol. 509. - P. 310-317. http://dx.doi.org/10.1038/nature13085
- Knight G.E., Oliver-Redgate R., Burnstock G. Unusual absence of endothelium-dependent or -independent vasodilatation to purines or pyrimidines in the rat renal artery // Kidney Int. - 2003. - Vol. 64. - P. 1389-1397. http://dx.doi.org/10.1046/j.1523-1755.2003.00233.x
- Metcalfe M.J., Baker D.M., Turmaine M. et al. Alterations in purinoceptor expression in human long saphenous vein during varicose disease // Eur. J. Vasc. Endovasc. Surg. - 2007. - Vol. 33. - P. 239-250. http://dx.doi.org/10.1016/j.ejvs.2006.09.007
- Ralevic V. P2X receptors in the cardiovascular system and their potential as therapeutic targets in disease // Curr. Med. Chem. - 2015. - Vol. 22. - P. 851-865. http://dx.doi.org/10.2174/0929867321666141215094050
- Ralevic V., Burnstock G. Involvement of purinergic signaling in cardiovascular diseases // Drug News Perspect. - 2003. - Vol. 16. - P. 133-140. http://dx.doi.org/10.1358/dnp.2003.16.3.876886
- Schachter M. Endothelium and smooth muscle: trophic interactions and potential for therapeutic intervention // J. Hum. Hypertens. - 1990. - Vol. 4, suppl. 4. - P. 17-20; discussion 20-1.
- Turner C.M., Vonend O., Chan C. et al. The pattern of distribution of selected ATP-sensitive P2 receptor subtypes in normal rat kidney: an immunohistological study // Cells Tissues Organs. - 2003. - Vol. 175. - P. 105-117. http://dx.doi.org/10.1159/000073754
- Ziganshin A.U., Khaziakhmetov D.F., Ziganshina L.E. et al. Varicose disease affects the P2 receptor-mediated responses of human greater saphenous vein // Vascul. Pharmacol. - 2004. - Vol. 42. - P. 17-21. http://dx.doi.org/10.1016/j.vph.2004.11.007
- Ziganshin A.U., Ziganshin B.A. P2 receptors - Promising targets for future drugs // Current Topics in Pharmacol. - 2012. - Vol. 16. - P. 31-43.
- Ziganshina A.P., Ziganshin B.A., Ziganshin A.U. Dual effects of ATP on isolated arteries of the bovine eye // Pharmacol. Res. - 2012. - Vol. 66. - P. 170-176. http://dx.doi.org/10.1016/j.phrs.2012.04.002