Using the method of molecular genetic typing to determine variants of the weak antigen D in the diagnosis of Rh-affiliation
- Authors: Mineeva N.V.1, Gavrovskaya S.V.1, Sisoeva E.A.1, Bessmeltsev S.S.1, Sidorkevich S.V.1
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Affiliations:
- Russian Research Institute of Hematology and Transfusiology
- Issue: Vol 104, No 3 (2023)
- Pages: 325-331
- Section: Theoretical and clinical medicine
- URL: https://journals.rcsi.science/kazanmedj/article/view/145691
- DOI: https://doi.org/10.17816/KMJ119540
- ID: 145691
Cite item
Abstract
Background. Determination of Rh-affiliation is mandatory for donors and recipients, surgical patients, pregnant women, etc. There are variants of the D antigen that are difficult to identify by serological methods, for example, a weak D antigen.
Aim. Determination of Rh-affiliation using genotyping in difficult cases, when the use of serological methods does not allow obtaining a reliable result.
Material and methods. We studied blood samples from donors (n=18), pregnant women (n=17) and patients with hematological diseases (n=15): 22 men and 28 women, median age 36 years (25 to 54 years). Serologically, antigen D was determined by gel technology in ID-cards and in an indirect antiglobulin test with anti-D-IgG reagent. Weak D antigen variants were diagnosed and phenotype determined using polymerase chain reaction with allele-specific primers. For significance of differences in the frequency of types of weak antigen D, a nonparametric statistical method using a two-tailed Fisher's exact test was used. Differences were considered statistically significant at p <0.05.
Results. The use of genotyping made it possible to detect the presence of a weak antigen D in 41 samples. Its specificity was represented by the following types: 1; 1.1; 2; 3. Other types of weak antigen D [4; 4.0; 4.1; 4.2 (DAR); 5; 11; 14; 15; 17] were absent. The study of the phenotype of erythrocyte antigens of the Rhesus system using genotyping revealed the predominance of the Ccee phenotype in people with a weak D antigen. Significant differences in the frequency of types of this antigen were revealed.
Conclusion. The use of molecular genetic typing made it possible to determine the types of the weak antigen D and to accurately determine the Rh-affiliation of the subjects.
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##article.viewOnOriginalSite##About the authors
Natalia V. Mineeva
Russian Research Institute of Hematology and Transfusiology
Author for correspondence.
Email: izoserologia@mail.ru
ORCID iD: 0000-0001-7137-8877
D. Sci. (Biol.), Prof., Manager, Research Laboratory of Immunohematology and Genomics of Blood Groups
Russian Federation, St. Petersburg, RussiaSvetlana V. Gavrovskaya
Russian Research Institute of Hematology and Transfusiology
Email: izoserologia@mail.ru
ORCID iD: 0000-0003-2987-2956
Senior Researcher, Research Laboratory of Immunohematology and Genomics of Blood Groups
Russian Federation, St. Petersburg, RussiaElena A. Sisoeva
Russian Research Institute of Hematology and Transfusiology
Email: izoserologia@mail.ru
ORCID iD: 0000-0002-9465-4704
Senior Researche, Research Laboratory of Immunohematology and Genomics of Blood Groups
Russian Federation, St. Petersburg, RussiaStanislav S. Bessmeltsev
Russian Research Institute of Hematology and Transfusiology
Email: bessmeltsev@yandex.ru
ORCID iD: 0000-0001-7280-7100
M.D., D. Sci. (Med.), Prof., Deputy Director
Russian Federation, St. Petersburg, RussiaSergey V. Sidorkevich
Russian Research Institute of Hematology and Transfusiology
Email: bloodsciense@mail.ru
ORCID iD: 0000-0001-9931-9406
M.D., D. Sci. (Med.), Director
Russian Federation, St. Petersburg, RussiaReferences
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