电邮这篇文章 ABC of the future: prolonged coculture of human embryos with endometrium in a nutrient medium in vitro
- 作者: Bespalova O.1, Komarova E.1, Lesik E.2, Ob’edkova K.3, Tapilskaya N.3, Kogan I.1
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隶属关系:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D. O. Ott
- 期: 卷 73, 编号 3 (2024)
- 页面: 19-25
- 栏目: Original Research
- URL: https://journals.rcsi.science/jowd/article/view/261037
- DOI: https://doi.org/10.17816/JOWD629131
- ID: 261037
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BACKGROUND: The development of new systems for the cultivation of embryos of model objects and the creation of embryonic models based on stem cells allow for describing the events of early embryogenesis in more detail. However, these models have a number of limitations, endorsing only indirect extrapolation of the knowledge gained to events occurring in vivo. Despite certain limitations, including those related to some differences between embryos developing in vivo and in vitro, implantation conditions in vivo and in vitro, and limited time for culturing human embryos, the study of post-implantation development has great prospects.
AIM: The aim of this study was to evaluate the viability and potential for the development of human embryos from the blastocyst stage during their prolonged coculture with the endometrium in culture media designed to support the growth to the blastocyst stage.
MATERIALS AND METHODS: Embryos obtained through assisted reproductive technology were cultured from the blastocyst stage to day 19st of development in culture media designed to support the growth to the blastocyst stage, in the presence of the endometrium. On day 19st, embryos and endometrial fragments were assessed under an inverted microscope using Hoffman modulation contrast.
RESULTS: During visual assessment under an inverted microscope on day 19st of development and days 13–14st of coculture with the endometrium, viable developing embryos without signs of degradation were recorded, both freely located in a mass of undifferentiated cells and in direct contact with the endometrium.
CONCLUSIONS: The data obtained indicate the ability of the embryo to further develop in the presence of the endometrium from the blastocyst stage to day 19st in in culture media that supports the growth to the blastocyst stage. This may serve as an experimental model for both evaluating endometrial receptivity in vitro and studying intercellular communication during implantation.
作者简介
Olesya Bespalova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: shiggerra@mail.ru
ORCID iD: 0000-0002-6542-5953
SPIN 代码: 4732-8089
MD, Dr. Sci. (Med.)
俄罗斯联邦, Saint PetersburgEvgenia Komarova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
编辑信件的主要联系方式.
Email: evgmkomarova@gmail.com
ORCID iD: 0000-0002-9988-9879
SPIN 代码: 1056-7821
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgElena Lesik
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: lesike@yandex.ru
ORCID iD: 0000-0003-1611-6318
SPIN 代码: 6102-4690
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgKsenia Ob’edkova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D. O. Ott
Email: obedkova_ks@mail.ru
ORCID iD: 0000-0002-2056-7907
SPIN 代码: 2709-2890
MD, Cand. Sci. (Med.)
俄罗斯联邦, Saint PetersburgNatalia Tapilskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D. O. Ott
Email: tapnatalia@yandex.ru
ORCID iD: 0000-0001-5309-0087
SPIN 代码: 3605-0413
MD, Dr. Sci. (Med.), Professor
俄罗斯联邦, Saint PetersburgIgor Kogan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
SPIN 代码: 6572-6450
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences
俄罗斯联邦, Saint Petersburg参考
- ISSCR. Guidelines for the conduct of human embryonic stem cell research. Version 1: December 21, 2006. [Internet]. [cited 2024 Feb 12]. Available from: https://www.forth.gr/_gfx/pdf/ISSCRhESCguidelines2006.pdf
- ISSCR. Guidelines for Stem Cell Research and Clinical Translation update. Version 1.0, May 2021. [Internet]. [cited 2024 Feb 12]. Available from: https://research.wisc.edu/wp-content/uploads/sites/2/2021/10/isscr-guidelines-for-stem-cell-research-and-clinical-translation-2021.pdf
- Bespalova ON, Kogan IY, Komarova EM, et al. The experience of extended in vitro human embryo cultivation in a culture medium containing endometrium cells. A pilot study. Journal of Obstetrics and Women’s Diseases. 2022;71(4):13–20. EDN: ZMYAAQ doi: 10.17816/JOWD109215
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- Weimar CH, Post Uiterweer ED, Teklenburg G, et al. In-vitro model systems for the study of human embryo-endometrium interactions. Reprod Biomed Online. 2013;27(5):461–476. doi: 10.1016/j.rbmo.2013.08.002
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