Comparison of laboratory diagnostic methods for urogenital infections caused by Chlamydia trachomatis
- Authors: Shalepo K.V.1, Shipitsina E.V.1, Savicheva A.M.1, Domeyka M.2,3
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Affiliations:
- Research Institute of Obstetrics and Gynecology named after D.O. Otta RAMS
- Institute of Clinical Bacteriology
- Uppsala University
- Issue: Vol 50, No 4 (2001)
- Pages: 77-82
- Section: Original Research
- URL: https://journals.rcsi.science/jowd/article/view/95656
- DOI: https://doi.org/10.17816/JOWD95656
- ID: 95656
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Abstract
According to the Russian-Swedish project there was performed a comparison of methods used for Chlamydia trachomatis detection in cervical samples, obtained from 397women and urethral samples from 253 men. All specimens were examined by direct immunofluorescence (DIF), polymerase chain reaction (PCR) and cell culture (CC). In high-prevalence group (group I) chlamydiae were detected in 17,8% and 28,0% of cases in men and women, respectively. Ingroup II containing patients who were subjected to screening examination, chlamydiae were found in 5% of cases both in men and women. PCR was shown to be the most sensitive when cervical samples in group I and cervical and urethral samples in group II were investigated. When urethral samples in group I were tested, DIF proved to have the highest sensitivity. All the methods used were found to be high specific. The search for standards of genital chlamydial infection diagnosis is in progress.
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##article.viewOnOriginalSite##About the authors
K. V. Shalepo
Research Institute of Obstetrics and Gynecology named after D.O. Otta RAMS
Author for correspondence.
Email: info@eco-vector.com
Russian Federation, Saint Petersburg
E. V. Shipitsina
Research Institute of Obstetrics and Gynecology named after D.O. Otta RAMS
Email: info@eco-vector.com
Russian Federation, Saint Petersburg
A. M. Savicheva
Research Institute of Obstetrics and Gynecology named after D.O. Otta RAMS
Email: info@eco-vector.com
Russian Federation, Saint Petersburg
M. Domeyka
Institute of Clinical Bacteriology; Uppsala University
Email: info@eco-vector.com
Switzerland, Uppsala