Email this article Diagnostic value of p16inkα/Ki-67 Dual-stained cytology in HPV-associated cervical diseases
- Authors: Khachaturyan A.R.1, Orekhova E.K.2, Tolibova G.K.3, Tral T.G.3
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Affiliations:
- I. P. Pavlov State Medical University of St. Petersburg
- EMC clinic
- D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS
- Issue: Vol 64, No 3 (2015)
- Pages: 47-51
- Section: Articles
- URL: https://journals.rcsi.science/jowd/article/view/1251
- DOI: https://doi.org/10.17816/JOWD64347-51
- ID: 1251
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Abstract
Human papillomavirus (HPV) infection is currently considered the most common of sexually transmitted infections. However, only 8-20 % of cases of infection with human papillomavirus oncogenic risk has the potential to transform to a neoplastic process. Detection of productive forms of HPV infection is the basis of early diagnosis of precancerous lesions. In order to clarify the severity of neoplasia and choose a sufficient way of treatment we investigated the diagnostic value of p16inkα/Ki-67 Dual-stained cytology method for assessing the presence and degree of expression of p16inkα protein and Ki-67, biomarkers of virus-induced proliferation, the sensitivity and positive predictive value of co-expression degree of which can increase the diagnostic value of cervical cytology.
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##article.viewOnOriginalSite##About the authors
Armine Robertovna Khachaturyan
I. P. Pavlov State Medical University of St. Petersburg
Email: armine2709@rambler.ru
associate professor of the chair of Obstetrics and Gynecology
Ekaterina Konstantinovna Orekhova
EMC clinic
Email: orekhovakatherine@gmail.com
оbstetrician-gynecologist
Gulrukhsor Khaybulloevna Tolibova
D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS
Email: gulyatolibova@mail.ru
PhD. MD. Senior Scientist. Laboratory of Cell Biology, Department of Pathomorphology
Tatyana Georgiyevna Tral
D. O. Ott Research Institute of Obstetrics and Gynecology, RAMS
Email: TTG2008@bk.ru
MD. Pathologist. Department of Pathomorphology
References
- Александрова Ю. Н., Лыщев А. А., Сафронникова Н. Р. Папилломавирусная инфекция у здоровых женщин Санкт-Петербурга. Вопросы онкологии. 2000; 2: 175-9.
- Андосова Л. Д., Качалина О. В., Куделькина С. Ю., Гонова Е. С. Генодиагностика папилломавирусной инфекции у женщин с различной патологией репродуктивной сферы. Медицинский альманах. 2012; 2 (12): 71-3.
- Андосова Л. Д., Конторщикова К. Н., Качалина О. В. Методы ранней диагностики и новые скрининговые технологии при заболеваниях шейки матки. Медицинский альманах. 2011; 6 (19): 98-102.
- Роговская С. И., Михеева И. В., Шипулина О. Ю., Минкина Г. Н., Подзолкова Н. М., Радзинский В. Е., Шипулин Г. А. Распространенность папилломавирусной инфекции в России. Эпидемиология и вакцинопрофилактика. 2012; 1: 25-34.
- Савичева А. М., Шипицина Е. В. Вакцинация и скрининг: интеграция стратегий профилактики рака шейки матки. Журнал акушерства и женских болезней. 2009; 5: 90-1.
- Шипицына Е. В., Оржесковская Е. А., Бабкина К. А., Савичева А. М., Микая Н. А., Орлова О. О., Юркова И. К. Определение вирусной нагрузки и статуса ДНК вируса папилломы человека 16 типа методом ПЦР в реальном времени. Журнал акушерства и женских болезней. 2004; 4: 26-32.
- Abbott Molecular RealTime High Risk HPV Optimising Cervical Cancer Screening. Available at: http://www.abbottmolecular.com/products/infectious-diseases/realtime-pcr/hepatitis-high-risk-hpv-assay.html.
- Bosh F. X. Human papillomavirus: science and technologies for the elimination of cervical cancer. Expert Opin Pharmacother. 2011; 12 (14): 2189-204.
- Digene Corporation Hybrid Capture® 2 High-Risk HPV DNA TestTM, An In Vitro Nucleic Acid Hybridization Assay with Signal Amplification using Microplate Chemiluminescence for the Qualitative Detection of Human Papillomavirus (HPV) Types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 in Cervical Specimens. 2004; 10.
- Franceschi S., Herrero R., Clifford G. M., et al. Variations in the age - specific curves of human papillomavirus prevalence in wmen wordwide. Int J Cancer. 2006; 119: 2677-84.
- Ogunmodede F., MD; Steven H. Yale et al. Human Papillomavirus Infections in Primary Care. Clinical Medicine Research. 2007; 5 (4): 210-7.
- Sedlacek T. V., Lindhein S. et al. Mechanism for HPV transmission at birth. Am. J. Obstet. Gynecol. 1989; 161: 55-9.
- World Health Organization, International Agency of Research on Cancer. Handbooks of Cancer Prevention. Cervix Cancer Screening. Geneva: IARC Press, 2005.
