Gas chromatography-mass spectrometry based metabolomics of urine steroids in women with different phenotypes of polycystic ovary syndrome

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Abstract

This article presents an analysis of the metabolism of androgens, glucocorticoid hormones, and progestins obtained  by gas chromatography-mass spectrometry in 56 women with obesity and hyperandrogenia in comparison with 23 women with alimentary-constitutional obesity and 25 healthy women. An increase in urinary excretion of androsterone, 16-oxo-androstenediol (16-oxo-dA2), and α-tetrahydrometabolites of cortisone and corticosterone has been established in 25 women with polycystic-altered ovaries according to ultrasound, with urinary excretion of dehydroepiandrosterone (DHEA) and its metabolites (dA2-17β, 16β-DHEA, 16-oxo-dA2, and androstentriol), α- and β-metabolites of glucocorticoids and androstenedione, and 5-en-pregnans increased in 31 women without signs of polycystic ovaries according to ultrasound. A decrease in the tetrahydrocortisol/tetrahydrocortisone ratio was found in women with polycystic ovaries, indicating a decrease in the activity of 11β-hydroxysteroid dehydrogenase type 1. Evidence for the increased 5α-reductase activity was obtained in women with different phenotypes of polycystic ovary  syndrome.

About the authors

Mariya V. Matyushenko

North-Western State Medical University named after I.I. Mechnikov

Author for correspondence.
Email: tatarinova.maria@mail.ru

Post-Graduate Student

Russian Federation, Saint Petersburg

Nataliya V. Vorokhobina

North-Western State Medical University named after I.I. Mechnikov

Email: natalya.vorokhobina@szgmu.ru

MD, PhD, DSci (Medicine), Professor, the Head of the Department of Endocrinology named after V.G. Baranov

Russian Federation, Saint Petersburg

Lyudmila I. Velikanova

North-Western State Medical University named after I.I. Mechnikov

Email: velikanova46@gmail.com

PhD, DSci (Biology), Professor, the Head of the Research Laboratory of Chromatography

Russian Federation, Saint Petersburg

Yuliya V. Kovalyova

North-Western State Medical University named after I.I. Mechnikov

Email: yuliya_kovalyova@inbox.ru

PhD, Associate Professor

Russian Federation, Saint Petersburg

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2. Fig. 1. Signs of decreased activity of 11β-hydroxysteroid dehydrogenase in women with polycystic ovary syndrome (PCOS) with phenotype A: ТНF — tetrahydrocortisol; THE — tetrahydrocortisone; CL — α + β-cortols; CN — α + β-cortolones

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3. Fig. 2. Signs of increased activity of 5α-reductase in women with polycystic ovary syndrome (PCOS) with phenotypes А and B: Аn — androsterone; Et — ethiocholanolone; THF — tetrahydrocortisol; THB — tetrahydrocorticosterone

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Copyright (c) 2018 Matyushenko M.V., Vorokhobina N.V., Velikanova L.I., Kovalyova Y.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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