Compared diagnostic value of proteinuria definition applying different methods at pregnants with preeclampsia

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Abstract

Colorimetric method of protein determination in urine with use of pyrogallol red (PGR) has certain advantages over a routine method of proteinuria determination with use of sulfosalicylic acid (SSA) which is still applied in most clinics in the Russian Federation. For the purpose to determinate the diagnostic significance of proteinuria estimation by different methods at pregnants with preeclampsia, conducted a comparative research of proteinuria levels at 68 pregnants with preeclampsia and at 15 healthy pregnant by two methods: with sulfosalicylic acid and with pyrogallol red. At the same patients the diagnostic significance of protein/creatinine ratio was evaluated when using the method with PGR. It was revealed that proteinuria determination with PGR- method had authentically higher sensitivity, and proteinuria indices when using this method corresponds to the criteria for evaluation of preeclampsia severity specified in the Ministry of Health Russian Federation Order No. 572n 2012 and the Clinical Recomandations “Hypertensia during pregnancy. Preeclampsia. Eclampsia” (2012). In case of proteinuria determination by method with SSK it is necessary to consider lower sensitivity of the method and adhere to earlier classification of preeclampsia (gestosis) where proteinuria more 1 g/l was regarded as the symptom of severe preeclampsia. Confirmation of the diagnostic significance of protein/creatinine ratio needs further researches.

About the authors

Elena V. Mozgovaya

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”; St. Department of Obstetrics, Gynecology and Reproductology

Author for correspondence.
Email: elmozg@mail.ru

doctor of Medical Sciences, leading scientific researcher, the head 3th department of pathology of pregnancy

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034; Saint Petersburg

Natalya E. Androsova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: iagmail@ott.ru

chief of clinical laboratory

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Vladimir V. Dorofeicov

Department of Biochemistry Lesgaft National State University of Physical Education, Sport and Health

Email: vdorofeykov@yandex.ru

head of biochemistry department

Russian Federation, Saint Petersburg

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Copyright (c) 2017 Mozgovaya E.V., Androsova N.E., Dorofeicov V.V.

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