Peculiarities of placental microRNA expression in pregnancies complicated by gestational diabetes mellitus and preeclampsia

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Abstract

The aim of study was to determine feasible changes of placental miRNAs expression profiles revealed by next generation sequencing (NGS) in pregnancies with GDM complicated or not with PE. Out of 27 miRNAs, studied expression was significantly different (FDR < 0.05) only for his-miR-45a. Comparative analysis of revealed reliable differences in expression of hsa-miR-4532 (p < 0.0001, FDR = 0.0008), hsa-miR-34c-5p (p < 0.0001, FDR = 0.0083), and hsa-miR-193b-5p (p < 0.0001, FDR = 0.0139) in pregnancy complicated by PE, without of GDM. The present results suggest that GDM and PE are associated with specific alterations in the placental miRNA expression profiles. Further studies are needed to verify the role of these microRNA in molecular mechanisms underlying GDM and PE pathogenesis.

About the authors

Vladimir S. Pakin

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Author for correspondence.
Email: pakins@list.ru

PhD student

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Elena S. Vashukova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: vi_lena@list.ru

scientist

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Roman V. Kapustin

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: kapustin.roman@gmail.com

PhD

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Olga N. Arzhanova

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: arjanova_olga@mail.ru

head of the Department Pathology of pregnancy, professor

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Andrey S. Glotov

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: anglotov@mail.ru

senior researcher of the Laboratory prenatal diagnosis of hereditary and congenital diseases

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

Vladislav S. Baranov

FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”

Email: baranov@vb2475.spb.ed

head of the Laboratory prenatal diagnosis of hereditary and congenital diseases, Corr. RAS, Professor

Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

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Copyright (c) 2017 Pakin V.S., Vashukova E.S., Kapustin R.V., Arzhanova O.N., Glotov A.S., Baranov V.S.

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