Cytokine profile in women with threatened miscarriage and intestinal dysbiosis
- Authors: Bezmenko A.A.1, Sadovaya N.D.1, Meshkova M.E.1, Malakhova E.A.1
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Affiliations:
- Military Medical Academy named after S.M. Kirov
- Issue: Vol 70, No 1 (2021)
- Pages: 51-58
- Section: Original study articles
- URL: https://journals.rcsi.science/jowd/article/view/48554
- DOI: https://doi.org/10.17816/JOWD48554
- ID: 48554
Cite item
Abstract
HYPOTHESIS/AIMS OF STUDY: Systemic endotoxinemia resulting from intestinal dysbiosis activates the Th1 immune response and excessive production of proinflammatory cytokines, which can cause abortion. This study was aimed at assessing interleukin levels in women with the threat of miscarriage and exploring their dependence on intestinal microbiocenosis and the level of endotoxinemia.
STUDY DESIGN, MATERIALS AND METHODS: The study involved 87 women aged 18 to 43 years in pregnancy from six to 22 weeks. The main group consisted of 50 women with a threatened miscarriage. The control group included 37 women with normal pregnancy. A qualitative and quantitative analysis of intestinal microbiocenosis was performed by real-time PCR, with endotoxin and interleukin levels evaluated using conventional methods.
RESULTS: In patients of the main group, intestinal dysbiosis of grade I was detected in 30 % (n = 15), of grade II in 46 % (n = 23), and of grade III in 24 % (n = 12) of cases. In the control group, intestinal microflora disorders corresponded to grade I dysbiosis in 67.6 % (n = 25) and grade II dysbiosis in 32.4 % (n = 12) of cases, there being no cases of severe dysbiosis revealed. The level of endotoxin in pregnant women of the main group was 0.57 ± 0.02 nmol / ml and was classified as “increased”. The endotoxin level in the blood serum of pregnant women of the control group was 0.34 ± 0.02 nmol / ml and was characterized as “low” (p < 0.001). A strong correlation was found (r = 0.8, p < 0.001) between the grade of intestinal dysbiosis and the level of endotoxinemia. The cytokine profile in patients of the main group was characterized by increased concentrations of pro-inflammatory cytokines (IL-1β — 4.9 ± 1.6 pg / ml, IL-6 — 4.8 ± 1.5 pg / ml) and a decreased concentration of anti-inflammatory cytokine (IL-10 — 18.0 ± 4.5 pg / ml), when compared to the control group (IL-1β — 1.8 ± 0.2 pg / ml, IL-6 — 2.1 ± 0.2 pg / ml, IL-10 — 30.3 ± 4.4 pg/ml). There were a statistically significant moderate positive correlation between the level of endotoxinemia and the concentration of pro-inflammatory cytokines and a weak negative correlation between the endotoxin level and the concentration of anti-inflammatory IL-10.
CONCLUSION: Endotoxinemia, which occurs as a result of intestinal microflora dysbiosis and activates pro-inflammatory pathways, can really be as a triggering factor in the pathogenesis of miscarriage in pregnant women.
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##article.viewOnOriginalSite##About the authors
Alexander A. Bezmenko
Military Medical Academy named after S.M. Kirov
Email: bezmenko@yandex.ru
ORCID iD: 0000-0003-2837-1260
MD, PhD, Assistant Professor
Russian Federation, Saint-PetersburgNatalia D. Sadovaya
Military Medical Academy named after S.M. Kirov
Author for correspondence.
Email: nataliacrimea@gmail.com
ORCID iD: 0000-0003-3362-4402
SPIN-code: 7063-7367
MD
Russian Federation, Saint-PetersburgMarina E. Meshkova
Military Medical Academy named after S.M. Kirov
Email: meshkova.63@mail.ru
ORCID iD: 0000-0002-0694-8586
SPIN-code: 6465-3776
PhD in Biology, Senior Lecture at the Department of Clinical Biochemistry and Laboratory Diagnostics
Russian Federation, Saint-PetersburgElena A. Malakhova
Military Medical Academy named after S.M. Kirov
Email: elena196419@yandex.ru
ORCID iD: 0000-0002-9825-0408
SPIN-code: 7136-7795
Lecture at the Department of Clinical Biochemistry and Laboratory Diagnostics
Russian Federation, Saint-PetersburgReferences
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