Blood serum and follicular fluid relaxin: a pilot study of the hormone effects on ovarian function and fertilization efficiency
- Authors: Bespalova O.N.1, Zagaynova V.A.1, Kosyakova O.V.1, Gzgzyan A.M.1,2, Kogan I.Y.1,2, Milyutina Y.P.1, Tkachenko N.N.3, Borodina V.L.4, Lesik E.A.1, Mekina I.D.1, Komarova E.M.1
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Affiliations:
- The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- Saint Petersburg State University
- the «Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott"
- The «Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott»
- Issue: Vol 69, No 5 (2020)
- Pages: 59-68
- Section: Original Research
- URL: https://journals.rcsi.science/jowd/article/view/44441
- DOI: https://doi.org/10.17816/JOWD69559-68
- ID: 44441
Cite item
Abstract
Hypothesis/aims of study. To date, one of the most important avenues of research in the field of reproductive medicine is the searching for new biochemical markers of oocyte quality and the prediction of the effectiveness of in vitro fertilization (IVF) protocols. The aim of this study was to assess the effect of relaxin levels in blood serum and follicular fluid on the efficiency of ovulation stimulation, fertilization, and characteristics of the embryos.
Study design, materials and methods. This prospective randomized cohort study included 11 patients undergoing infertility treatment in a superovulation stimulation protocol using gonadotropin-releasing hormone antagonists. Age, body mass index, hormonal status, ovarian response, endometrial thickness and structure, the number and quality of oocytes and embryos, as well as fertilization efficiency were assessed. The level of relaxin in blood serum and follicular fluid samples was determined on the day of transvaginal follicle puncture using enzyme immunoassay.
Results. A correlation between follicular fluid relaxin levels and body mass index, age, the number of oocytes, and their fertilization efficiency (p < 0.05) was established. Changes in follicular fluid relaxin level were revealed depending on the gonadotropin preparations (p < 0.05) and triggers of final maturation of oocytes (p < 0.05). The tendency of the effect of gonadotropin doses on circulating relaxin levels, and of the hormone itself on endometrial thickness and the quality of oocytes was determined.
Conclusion. Determination of the relaxin concentration can be considered as a promising method for predicting the result of ovarian stimulation and the efficiency of fertilization in IVF protocols.
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##article.viewOnOriginalSite##About the authors
Olesya N. Bespalova
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: shiggerra@mail.ru
ORCID iD: 0000-0002-6542-5953
SPIN-code: 4732-8089
MD, PhD, DSci (Medicine), Deputy Director
Russian Federation, Saint PetersburgValeriya A. Zagaynova
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Author for correspondence.
Email: zagaynovav.al.52@mail.ru
ORCID iD: 0000-0001-6971-7024
SPIN-code: 7409-4944
MD, Post-Graduate Student. The Assisted Reproduction Technology Department
Russian Federation, Saint PetersburgOlga V. Kosyakova
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: for.olga.kosyakova@gmail.com
ORCID iD: 0000-0003-4116-0222
MD, Post-Graduate Student. The Department of Pathology of Pregnancy N 1
Russian Federation, Saint PetersburgAlexander M. Gzgzyan
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State University
Email: ovrt@ott.ru
ORCID iD: 0000-0003-3917-9493
MD, PhD, DSci (Medicine), Head of the Department of Reproductology, Head of the Assisted Reproduction Technology Department; Professor. The Department of Obstetrics, Gynecology, and Reproductive Sciences, the Faculty of Medicine
Russian Federation, Saint PetersburgIgor Yu. Kogan
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State University
Email: ovrt@ott.ru
ORCID iD: 0000-0002-7351-6900
MD, PhD, DSci (Medicine), Professor, Corresponding Member of RAS, Director; Professor. The Department of Obstetrics, Gynecology, and Reproductive Sciences, the Faculty of Medicine
Russian Federation, Saint PetersburgYulia P. Milyutina
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: milyutina1010@mail.ru
ORCID iD: 0000-0003-1951-8312
PhD, Chairman of the Council of Young Scientists
Russian Federation, Saint PetersburgNatalia N. Tkachenko
the «Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott"
Email: iagmail@ott.ru
ORCID iD: 0000-0001-6189-3488
PhD, Head of the Laboratory of Endocrinology
Russian Federation, Saint PetersburgValentina L. Borodina
The «Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott»
Email: iagmail@ott.ru
Laboratory Assistant. The Laboratory of Endocrinology
Russian Federation, Saint Petersburg State UniversityElena A. Lesik
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: ovrt@ott.ru
ORCID iD: 0000-0003-1611-6318
PhD, Senior Researcher. The Laboratory of Early Embryogenesis; Embryologist. The Assisted Reproduction Technology Department
Russian Federation, Saint PetersburgIrina D. Mekina
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: ovrt@ott.ru
ORCID iD: 0000-0002-0813-5845
PhD, Senior Researcher. The Laboratory of Early Embryogenesis; Senior Embryologist. The Assisted Reproduction Technology Department
Russian Federation, Saint PetersburgEvgenia M. Komarova
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: ovrt@ott.ru
ORCID iD: 0000-0002-9988-9879
PhD, Head of the Laboratory of Early Embryogenesis, Embryologist. The Assisted Reproduction Technology Department
Russian Federation, Saint PetersburgReferences
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