Endometrial stem cells expansion capability for local and systemic routes of administration in a model of experimentally injured endometrium

Cover Page


Cite item

Full Text

Abstract

Endometrial stem cells due to their therapeutic characteristics could to be an effective tool of cell technologies in reproductive medicine. The aim of the study was to determine the most therapeutically effective route of administration for endometrial stem cells suspension. The study was conducted in approved animal model of injured endometrium. To create the experimental model tissue pieces of autologous endometrium were implanted on the anterior abdominal wall peritoneum using general surgical techniques. Experimental group animals were treated with endometrial stem cells suspension; in the control animal group a placebo was used. Local and systemic routes of endometrial stem cells administration were compared. The direct injections of stem cells suspension in the endometrial implants were used as the local route of administration, the intravenous injections of stem cells suspension were used as a systemic route. Endometrial stem cells expansion didn’t depend on the routes of administration, whereas therapeutic effects of stem cells was more obvious in tissue pieces after local injection of stem cells.

About the authors

Elena O Usoltceva

St. Petersburg State University

Email: usolceva.elena@inbox.ru
MD. St. Faculty of Medicine, Department of Obstetrics, Gynecology and Reproductive Medicine

Liailia Kh Dzhemlikhanova

St. Petersburg State University

Email: office@inform.pu.ru
MD, Associate Professor of Obstetrics, Gynecology and Reproductive Medicine Department. Faculty of Medicine

Dariko A Niauri

St. Petersburg State University

Email: office@inform.pu.ru
MD, Professor, Head of Obstetrics, Gynecology and Reproductive Medicine Department

Igor Yu Kogan

D.O. Ott Research Institute of Obstetrics and Gynecology RAMS

Email: iagmail@ott.ru
MD, academic secretary

Alexander M Gzgzyan

St. Petersburg State University

Email: iagmail@ott.ru
MD, Professor of Obstetrics, Gynecology and Reproductive Medicine Department, Head of Reproductive Technologies Department. Faculty of Medicine

References

  1. Stoltz JF, de Isla N, Li YP, et al. Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century. Stem Cells Int. 2015;2015:734-731. doi: 10.1155/2015/734731.
  2. Земелько В.И., Гринчук Т.М., Домнина А.П., и др. Мультипотентные мезенхимные стволовые клетки десквамированного эндометрия. Выделение, характеристика и использование в качестве фидерного слоя для культивирования эмбриональных стволовых линий человека // Цитология. - 2011. - № 12. - С. 919-928. [Zemel’ko VI, Grinchuk TM, Domnina AP, et al. Multipotent mesenchymal stem cells out of desquamated endometrium. Isolation, characterization, and usage as a feeder layer for the cultivation of human embryonic stem lines. Tsitologiya. 2011;12:919-928. (In Russ).]
  3. Murakami K, Bhandari H, Lucas ES, et al. Deficiency in clonogenic endometrial mesenchymal stem cells in obese women with reproductive failure a pilot study. PLoS One. 2013 Dec 10;8(12): e82582. doi: 10.1371/journal.pone.0082582.
  4. Djokovic D, Calhaz-Jorge C. Somatic stem cells and their dysfunction in endometriosis. Front Surg. 2015 Jan 6;1:51. doi: 10.3389/fsurg.2014.00051.
  5. Zhong Z, Patel AN, Ichim TE, et al. Feasibility investigation of allogeneic endometrial regenerative cells. J Transl Med. 2009;7:15. doi: 10.1186/1479-5876-7-15.
  6. Ichim TE, Solano F, Lara F, et al. Combination stem cell therapy for heart failure. Int Arch Med. 2010 Apr 14;3(1):5. doi: 10.1186/1755-7682-3-5.
  7. Ichim TE, Alexandrescu DT, Solano F, et al. Mesenchymal stem cells as antiinflammatories: implications for treatment of Duchenne muscular dystrophy. Cell Immunol. 2010;260(2):75-82. doi: 10.1016/j.cellimm.2009.10.006.
  8. Айламазян Э.К., Гзгзян А.М., Савинов П.А., и др. Особенности реакции импланта аутологичного эндометрия на механическое воздействие в эксперименте // Журнал акушерства и женских болезней. - 2012. - № 3. - C. 22-30. [Ailamazian EK, Gzgzian AM, Savinov PA, et al. The endometrial implant’s reaction after the experimental mechanical tissue damage. Zhurnal akusherstva i zhenskikh boleznei. 2012;3:22-30. (In Russ).]
  9. Айламазян Э.К., Гзгзян А.М., Джемлиханова Д.Х., и др. Опыт получения стволовых клеток из менструальной крови // Журнал акушерства и женских болезней. - 2012. - № 5. - C. 25-29. [Aylamazyan EK, Gzgzyan AM, Dzhemlikhanova DKh, et al. The experience of obtaining stem cells from menstrual blood. Zhurnal akusherstva i zhenskikh bolezney. 2012;5:25-29. (In Russ).]
  10. Golpanian S, Schulman IH, Ebert RF, et al. Concise review: review and perspective of cell dosage and routes of administration from preclinical and clinical studies of stem cell therapy for heart disease. Stem Cells Transl Med. 2016 Feb;5(2):186-91. doi: 10.5966/sctm.2015-0101.
  11. Du G, Liu Y, Dang M, et al. Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats. Acta Histochem. 2014 Jul;116(6):1075-84. doi: 10.1016/j.acthis.2014.05.002.
  12. Suncion VY, Ghersin E, Fishman JE, et al. Does transendocardial injection of mesenchymal stem cells improve myocardial function locally or globally? An analysis from the POSEIDON randomized trial. Circ Res. 2014 Apr 11;114(8):1292-301. doi: 10.1161/CIRCRESAHA.114.302854.
  13. Leibacher J, Henschler R. Biodistribution, migration and homing of systemically applied mesenchymal stem/stromal cells. Stem Cell Res Ther. 2016 Jan 11;7(1):7. doi: 10.1186/s13287-015-0271-2.
  14. Кругляков П.В., Лохматова Е.А., Климович В.Б., Зарицкий А.Ю. Мезенхимальные стволовые клетки и иммунопатологические состояния организма // Клеточная трансплантология и тканевая инженерия. - 2006. - № 3 (5). - C. 36-41. [Kruglyakov PV, Lokhmatova EA, Klimovich VB, Zaritskiy AYu. Mesenchymal stem cells and immunopathological conditions. Kletochnaya transplantologiya i tkanevaya inzheneriya. 2006;3(5):36-41. (In Russ).]
  15. Брюховецкий И.С., Брюховецкий А.С., Мищенко П.В., Хотимченко Ю.С. Роль системных механизмов миграции и хоуминга стволовых клеток в развитии злокачественных опухолей центральной нервной системы и разработке новых методов противоопухолевой терапии // Российский биотерапевтический журнал. - 2013. - № 3. - C. 3-12. [Brjuhoveckij IS, Brjuhoveckij AS, Mishhenko PV, Hotimchenko JS. The role of systemic mechanisms of stem cells migration and homing in the development of malignant tumors of the central nervous system and the development of new anti-tumor therapies. Rossijskij bioterapevticheskij zhurnal. 2013;3:3-12. (In Russ).]
  16. Han X, Meng X, Yin Z, et al. Inhibition of intracranial glioma growth by endometrial regenerative cells. Cell Cycle. 2009 Feb 15;8(4):606-10. doi: 10.4161/cc.8.4.7731.
  17. Coughlan C, Ledger W, Wang Q, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online. 2014 Jan;28(1):14-38. doi: 10.1016/j.rbmo.2013.08.011.
  18. van Velthoven CT, Kavelaars A, van Bel F, Heijnen CJ. Nasal administration of stem cells: a promising novel route to treat neonatal ischemic brain damage. Pediatr Res. 2010 Nov;68(5):419-22. doi: 10.1203/pdr.0b013e3181f1c289.
  19. Henning RJ, Burgos JD, Vasko M, et al. Human cord blood cells and myocardial infarction: effect of dose and route of administration on infarct size. Cell Transplant. 2007;16(9):907-17. doi: 10.3727/096368907783338299.
  20. Panciani PP, Fontanella M, Tamagno I, et al. Stem cells based therapy in high grade glioma: why the intraventricular route should be preferred? J Neurosurg Sci. 2012 Sep;56(3):221-9.

Copyright (c) 2016 Usoltceva E.O., Dzhemlikhanova L.K., Niauri D.A., Kogan I.Y., Gzgzyan A.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies