The role of cervicovaginal microbiota in the occurrence of severe cervical intraepithelial dysplasia

Cover Page


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

BACKGROUND: Despite the availability of visual examination of the cervix, cervical screening in Russia has not been widespread enough, and therefore the incidence of cervical cancer remains consistently high. In recent years, the vaginal microbiota is given special attention as a risk factor for the development of precancerous diseases of the cervix.

AIM: The aim of this study was to compare the cervicovaginal microbiota in patients with high-grade squamous intraepithelial lesions (HSIL) or negative for intraepithelial lesions or malignancy (NILM).

MATERIALS AND METHODS: Microorganisms isolated from the cervicovaginal microbiota were identified in 40 patients using the mass spectrometry method. 20 women had severe cervical dysplasia, and the other 20 were healthy women without precancerous cervical lesions.

RESULTS: Corynebacterium spp. and Streptococcus spp. were found more often in the cervicovaginal microbiome in patients with high-grade squamous intraepithelial lesions than in women negative for intraepithelial lesions or malignancy.

CONCLUSIONS: The data obtained suggest that the presence of Corynebacterium spp. and Streptococcus spp. in the cervicovaginal microbiota is associated with the presence of severe cervical intraepithelial changes.

About the authors

Anna V. Kazakova

Samara State Medical University

Email: amigo14021980@yandex.ru
ORCID iD: 0000-0002-9483-8909
SPIN-code: 5129-5915

MD, Dr. Sci. (Med.), Assistant Professor

Russian Federation, Samara

Svetlana M. Chechko

Samara State Medical University

Author for correspondence.
Email: svetlana-chechko@mail.ru
ORCID iD: 0000-0002-3890-9944
SPIN-code: 1069-5588
Russian Federation, Samara

Evgeny F. Kira

Medical Academy, MEDSI Group of Companies

Email: profkira33@gmail.com
ORCID iD: 0000-0002-1376-7361
SPIN-code: 8955-9775

MD, Dr. Sci. (Med.), Professor

Russian Federation, Moscow

References

  1. World Health Organization [Internet]. Screening and treatment recommendations to prevent cervical cancer. Available from: https://www.who.int/news/item/06-07-2021-new-recommendations-for-screening-and-treatment-to-prevent-cervical-cancer [cited 2023 Sept 26.09].
  2. Rajaram S., Gupta B. Screening for cervical cancer: choices and dilemmas. Indian J Med Res. 2021;154(2):210–220. doi: 10.4103/ijmr.IJMR_857_20
  3. Kaprin AD, Starinskogo VV, Shaxzadovoĭ AO, editors. The state of cancer care for the population of Russia in 2021. Moscow: MNIOI im. P.A. Herzen – branch of the Federal State Budgetary Institution “National Medical Research Center of Radiology” of the Ministry of Health of Russia, 2022. (In Russ.)
  4. Pimple SA, Mishra GA. Global strategies for cervical cancer prevention and screening. Minerva Ginecol. 2019;71(4):313–320. doi: 10.23736/S0026-4784.19.04397-1
  5. Shmakova NA, Chistyakova GN, Kononova IN, et al. High oncogenic risk of cervical intraepithelial neoplasia and cervical cancer: the urgency of the problem, the search for prospects (literature review). Russian Journal of Human Reproduction. 2021;27(1):33-38. EDN: BGTJGG doi: 10.17116/repro20212701133
  6. Abakarova PR, Prilepskaya VN, Mezhevitinova EA, et al. Local cytokine therapy in a comprehensive treatment of HPV-associated cervical diseases. Gynecology. 2019;21(1):28–32. EDN: VYEBDU doi: 10.26442/20795696.2019.1.190284
  7. Cohen PA, Jhingran A, Oaknin A, et. al. Cervical cancer. Lancet. 2019;393(10167):169–182. doi: 10.1016/S0140-6736(18)32470-X
  8. Prilepskaya VN. HPV-associated cervical diseases: screening, methods of examination, principles of treatment. Gynecology. 2019;21(3):6–8. EDN: PRNMJI doi: 10.26442/20795696.2019.3.190595
  9. Aballéa S, Beck E, Cheng X, et al. Risk factors for cervical cancer in women in China: a meta-model. Womens Health. 2020;16. doi: 10.1177/1745506520940875
  10. Kira EF. Bacterial vaginosis. Moscow: MIA, 2012. (In Russ.) EDN: SZPGYL
  11. Borgogna JC, Shardell MD, Santori EK, et al. The vaginal metabolome and microbiota of cervical HPV-positive and HPV-negative women: a cross-sectional analysis. BJOG. 2020;127(2):182–192. doi: 10.1111/1471-0528.15981
  12. Smith BC, McAndrew T, Chen Z, et al. The cervical microbiome over 7 years and a comparison of methodologies for its characterization. PLoS One. 2012;7(7). doi: 10.1371/journal.pone.0040425
  13. Chen Y, Qiu X, Wang W, et al. Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort. BMC Infect Dis. 2020;20(1):629. doi: 10.1186/s12879-020-05324-9
  14. Gladysheva IV, Cherkasov SV. Corinebacteria of vaginal microbiom – potential pathogens or perspective probiotcs? Bulletin of the Orenburg Scientific Center of the Ural Branch of the Russian Academy of Sciences. 2019;(3):17. Available from: http://elmag.uran.ru:9673/magazine/Numbers/2019-3/Articles/GIV-2019-3.pdf. EDN: XRPVSW doi: 10.24411/2304-9081-2019-13022
  15. Soares GC, da Silva BA, Dos Santos MH, et al. Metallopeptidases produced by group B Streptococcus: influence of proteolytic inhibitors on growth and on interaction with human cell lineages. Int J Mol Med. 2008;22(1):119–125.
  16. Kang GU, Jung DR, Lee YH, et al. Potential association between vaginal microbiota and cervical carcinogenesis in korean women: a cohort study. Microorganisms. 2021;9(2):294. doi: 10.3390/microorganisms9020294
  17. Manzanares-Leal GL, Coronel-Martínez JA, Rodríguez-Morales M, et al. Preliminary Identification of the aerobic cervicovaginal microbiota in Mexican women with cervical cancer as the first step towards metagenomic studies. Front Cell Infect Microbiol. 2022;12. doi: 10.3389/fcimb.2022.838491
  18. Gladysheva IV, Chertkov KL, Cherkasov SV, et al. Probiotic potential, safety properties, and antifungal activities of corynebacterium amycolatum ICIS 9 and corynebacterium amycolatum ICIS 53 strains. Probiotics Antimicrob Proteins. 2023;15(3):588–600. doi: 10.1007/s12602-021-09876-3
  19. Makuza JD, Nsanzimana S, Muhimpundu MA, et al. Prevalence and risk factors for cervical cancer and pre-cancerous lesions in Rwanda. Pan Afr Med J. 2015;22:26. doi: 10.11604/pamj.2015.22.26.7116
  20. Mekonnen AG, Mittiku YM. Early-onset of sexual activity as a potential risk of cervical cancer in Africa: a review of literature. PLOS global public health. 2023;3(3). doi: 10.1371/journal.pgph.0000941
  21. Avgerinos KI, Spyrou N, Mantzoros CS, et al. Obesity and cancer risk: emerging biological mechanisms and perspectives. Metabolism. 2019;92:121–135. doi: 10.1016/j.metabol.2018.11.001
  22. Gnade CM, Hill EK, Botkin HE, et al. Effect of obesity on cervical cancer screening and outcomes. J Low Genit Tract Dis. 2020;24(4):358–362. doi: 10.1097/LGT.0000000000000570
  23. Ssedyabane F, Ngonzi J, Kajabwangu R, et al. Association between obesity and cervical intraepithelial neoplasia: results from a case control study in south western Uganda. BMC Women’s Health. 2023;23(1):159. doi: 10.1186/s12905-023-02315-1
  24. Nagelhout G, Ebisch RM, Van Der Hel O, et al. Is smoking an independent risk factor for developing cervical intra-epithelial neoplasia and cervical cancer? A systematic review and meta-analysis. Expert Rev Anticancer Ther. 2021;21(7):781–794. doi: 10.1080/14737140.2021.1888719
  25. Zhdan VM, Holovanova IA, Vovk OY, et al. relationship between cervical cancer and the level of preventive oncological examinations. Wiad Lek. 2021;74(6):1428–1432.
  26. Xiao T, Ou CQ, Yang J, et al. The Risk Factors for cervical cytological abnormalities among women infected with non-16/18 high-risk human papillomavirus: cross-sectional study. JMIR Public Health Surveill. 2022;8(12). doi: 10.2196/38628

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Presence of Streptococcus spp. depending on the presence and absence of cervical dysplasia in culture from the cervical canal and biopsy

Download (128KB)
Indexing metadata
3. Fig. 2. Presence of Corynebacterium spp. depending on the presence and absence of cervical dysplasia in culture from the cervical canal and biopsy

Download (133KB)
Indexing metadata

Copyright (c) 2024 Eсо-Vector

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).