Bacterial genera associated with nitrogen cycle in microbial communities of chernozems

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Abstract

BACKGROUND: Reactions associated with the geochemical nitrogen cycle occurring in the soil have a decisive effect on the level of nitrogen availability for green plants. Microorganisms that catalyze them are of interest from the point of view of developing mechanisms for managing soil fertility. In this paper, we focused on bacterial genera associated with nitrogen cycle processes in chernozem soils of the Belgorod Region of Russia.

AIM: To determine the list and quantitative ratios of bacterial genera whose representatives are able to participate in the fixation of atmospheric nitrogen, nitrification and denitrification in the chernozems of the forest-steppe zone of the European part of Russia.

MATERIALS AND METHODS: Samples of arable and non-arable chernozems of three subtypes were collected in June and August 2022. Microbiological profiling based on high-throughput sequencing of amplicons of V3 and V4 region of the 16S rRNA gene with subsequent computational processing of the results was used in the work.

RESULTS: 6 genera of nitrogen-fixing bacteria were found, as well as a group undifferentiated by means of the method used, including genus Rhizobium. 8 genera involved in the first and 5 genera involved in the second stage of nitrification were also found. 7 genera have been found whose representatives are capable of denitrification, without taking into account nitrogen-fixing bacteria, which are also capable of carrying out this process.

CONCLUSIONS: Among the bacteria capable of fixing atmospheric nitrogen in the chernozems of the Belgorod region, the genus Bradyrhizobium dominates. Among the nitrifiers performing the first stage of nitrification, Ellin6067 and MND1 from the family Nitrosomonadaceae predominate. The second stage of nitrification is carried out mainly by bacteria of the genus Nitrospira. Of the potential denitrifies, along with nitrogen-fixing genera possessing this ability, representatives of the genera Rubrobacter and Pseudomonas are the most numerous. The study does not claim to give a complete list of organisms that carry out key chemical transformations of nitrogen compounds in chernozems, however, it allows us to name their important participants.

About the authors

Konstantin S. Boyarshin

Belgorod State University

Email: ulmus-04@yandex.ru
ORCID iD: 0000-0002-2960-0670
SPIN-code: 6002-9327
Scopus Author ID: 57218822292
ResearcherId: HJZ-5162-2023

Cand. Sci. (Biology)

Russian Federation, Belgorod

Valeria V. Adamova

Belgorod State University

Email: adamova@bsu.edu.ru
ORCID iD: 0000-0001-8329-4670

Cand. Sci. (Biology)

Russian Federation, Belgorod

Wentao Zheng

Belgorod State University

Email: zhengwentaoo@126.com
ORCID iD: 0009-0003-3460-8401
Russian Federation, Belgorod

Ekaterina V. Nikitinskaya

Cherepovets State University

Email: nikitinskajacat@yandex.ru
Russian Federation, Cherepovets

Olga Y. Obukhova

Belgorod State University

Email: 1064261@bsu.edu.ru
ORCID iD: 0009-0007-5139-0394
Russian Federation, Belgorod

Marina V. Kolkova

Belgorod State University

Email: mvk3105@mail.ru
ORCID iD: 0009-0008-3849-3564
Russian Federation, Belgorod

Olga S. Bespalova

Belgorod State University

Email: olga9078@mail.ru
Russian Federation, Belgorod

Violetta V. Klyueva

Belgorod State University

Email: klyueva@bsu.edu.ru
ORCID iD: 0000-0002-9509-5115
Russian Federation, Belgorod

Kristina A. Degtyareva

Belgorod State University

Email: degtyareva@bsu.edu.ru
ORCID iD: 0000-0003-4474-0919
Russian Federation, Belgorod

Lubov V. Nesteruk

Belgorod State University

Email: nesteruk@bsu.edu.ru
ORCID iD: 0000-0003-3189-8178

Cand. Sci. (Biology)

Russian Federation, Belgorod

Yulia N. Kurkina

Belgorod State University

Email: kurkina@bsu.edu.ru
ORCID iD: 0000-0001-9180-1257

Cand. Sci. (Biology)

Russian Federation, Belgorod

Olesya A. Makanina

Belgorod State University

Email: makanina@bsu.edu.ru
ORCID iD: 0009-0006-7571-2493

Cand. Sci. (Biology)

Russian Federation, Belgorod

Elena S. Ivanova

Cherepovets State University

Email: stepinaelena@yandex.ru
ORCID iD: 0000-0002-6976-1452
Scopus Author ID: 57190344709

Cand. Sci. (Biology)

Russian Federation, Cherepovets

Irina V. Batlutskaya

Belgorod State University

Author for correspondence.
Email: bat@bsu.edu.ru
ORCID iD: 0000-0003-0068-6586

Dr. Sci. (Biology)

Russian Federation, Belgorod

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2. Fig. 1. Soil sampling points on the territory of the Belgorod Region of Russia

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3. Fig. 2. The ratio of the abundances of different ecological groups of bacteria — active participants in the nitrogen cycle in the chernozems of the Belgorod Region. Genera belonging to the same families are arranged nearby

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