Bacterial genera associated with nitrogen cycle in microbial communities of chernozems
- Authors: Boyarshin K.S.1, Adamova V.V.1, Zheng W.1, Nikitinskaya E.V.2, Obukhova O.Y.1, Kolkova M.V.1, Bespalova O.S.1, Klyueva V.V.1, Degtyareva K.A.1, Nesteruk L.V.1, Kurkina Y.N.1, Makanina O.A.1, Ivanova E.S.2, Batlutskaya I.V.1
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Affiliations:
- Belgorod State University
- Cherepovets State University
- Issue: Vol 22, No 2 (2024)
- Pages: 191-204
- Section: Ecosystems metagenomics
- URL: https://journals.rcsi.science/ecolgenet/article/view/262695
- DOI: https://doi.org/10.17816/ecogen603873
- ID: 262695
Cite item
Abstract
BACKGROUND: Reactions associated with the geochemical nitrogen cycle occurring in the soil have a decisive effect on the level of nitrogen availability for green plants. Microorganisms that catalyze them are of interest from the point of view of developing mechanisms for managing soil fertility. In this paper, we focused on bacterial genera associated with nitrogen cycle processes in chernozem soils of the Belgorod Region of Russia.
AIM: To determine the list and quantitative ratios of bacterial genera whose representatives are able to participate in the fixation of atmospheric nitrogen, nitrification and denitrification in the chernozems of the forest-steppe zone of the European part of Russia.
MATERIALS AND METHODS: Samples of arable and non-arable chernozems of three subtypes were collected in June and August 2022. Microbiological profiling based on high-throughput sequencing of amplicons of V3 and V4 region of the 16S rRNA gene with subsequent computational processing of the results was used in the work.
RESULTS: 6 genera of nitrogen-fixing bacteria were found, as well as a group undifferentiated by means of the method used, including genus Rhizobium. 8 genera involved in the first and 5 genera involved in the second stage of nitrification were also found. 7 genera have been found whose representatives are capable of denitrification, without taking into account nitrogen-fixing bacteria, which are also capable of carrying out this process.
CONCLUSIONS: Among the bacteria capable of fixing atmospheric nitrogen in the chernozems of the Belgorod region, the genus Bradyrhizobium dominates. Among the nitrifiers performing the first stage of nitrification, Ellin6067 and MND1 from the family Nitrosomonadaceae predominate. The second stage of nitrification is carried out mainly by bacteria of the genus Nitrospira. Of the potential denitrifies, along with nitrogen-fixing genera possessing this ability, representatives of the genera Rubrobacter and Pseudomonas are the most numerous. The study does not claim to give a complete list of organisms that carry out key chemical transformations of nitrogen compounds in chernozems, however, it allows us to name their important participants.
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##article.viewOnOriginalSite##About the authors
Konstantin S. Boyarshin
Belgorod State University
Email: ulmus-04@yandex.ru
ORCID iD: 0000-0002-2960-0670
SPIN-code: 6002-9327
Scopus Author ID: 57218822292
ResearcherId: HJZ-5162-2023
Cand. Sci. (Biology)
Russian Federation, BelgorodValeria V. Adamova
Belgorod State University
Email: adamova@bsu.edu.ru
ORCID iD: 0000-0001-8329-4670
Cand. Sci. (Biology)
Russian Federation, BelgorodWentao Zheng
Belgorod State University
Email: zhengwentaoo@126.com
ORCID iD: 0009-0003-3460-8401
Russian Federation, Belgorod
Ekaterina V. Nikitinskaya
Cherepovets State University
Email: nikitinskajacat@yandex.ru
Russian Federation, Cherepovets
Olga Y. Obukhova
Belgorod State University
Email: 1064261@bsu.edu.ru
ORCID iD: 0009-0007-5139-0394
Russian Federation, Belgorod
Marina V. Kolkova
Belgorod State University
Email: mvk3105@mail.ru
ORCID iD: 0009-0008-3849-3564
Russian Federation, Belgorod
Olga S. Bespalova
Belgorod State University
Email: olga9078@mail.ru
Russian Federation, Belgorod
Violetta V. Klyueva
Belgorod State University
Email: klyueva@bsu.edu.ru
ORCID iD: 0000-0002-9509-5115
Russian Federation, Belgorod
Kristina A. Degtyareva
Belgorod State University
Email: degtyareva@bsu.edu.ru
ORCID iD: 0000-0003-4474-0919
Russian Federation, Belgorod
Lubov V. Nesteruk
Belgorod State University
Email: nesteruk@bsu.edu.ru
ORCID iD: 0000-0003-3189-8178
Cand. Sci. (Biology)
Russian Federation, BelgorodYulia N. Kurkina
Belgorod State University
Email: kurkina@bsu.edu.ru
ORCID iD: 0000-0001-9180-1257
Cand. Sci. (Biology)
Russian Federation, BelgorodOlesya A. Makanina
Belgorod State University
Email: makanina@bsu.edu.ru
ORCID iD: 0009-0006-7571-2493
Cand. Sci. (Biology)
Russian Federation, BelgorodElena S. Ivanova
Cherepovets State University
Email: stepinaelena@yandex.ru
ORCID iD: 0000-0002-6976-1452
Scopus Author ID: 57190344709
Cand. Sci. (Biology)
Russian Federation, CherepovetsIrina V. Batlutskaya
Belgorod State University
Author for correspondence.
Email: bat@bsu.edu.ru
ORCID iD: 0000-0003-0068-6586
Dr. Sci. (Biology)
Russian Federation, BelgorodReferences
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