The ability of photochemical decomposition products of the Roundup to induce oxidative stress in bacterial cells

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Abstract

BACKGROUND: A common non-selective systemic herbicide Roundup (Glyphosate, active ingredient N-phosphonomethylglycine, N-PMG) is used to control perennial weeds. It is necessary to assess the hazard of the products of photochemical decomposition of N-FMG formed under the influence of solar UV and ozone.

AIM: Using lux-biosensors based on Escherichia coli, studying the ability of N-FMG photochemical degradation products to induce oxidative stress in bacterial cells.

MATERIALS AND METHODS: The work used the active substance of the herbicide Roundup N-phosphonomethylglycine (N-PMG), biosensors E. coli (pSoxS-lux), E. coli (pKatG-lux). UV radiation, Mass spectrometry.

RESULTS: Using biosensors, it was shown that the products of photochemical decomposition of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) cause an increase in the concentration of superoxide anion radical and H2O2 in E. coli cells, which induces oxidative stress in the bacterial cell.

CONCLUSIONS: The photochemical decomposition product of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) induces the formation of superoxide anion radical and H2O2 in bacterial cells.

About the authors

Elena A. Saratovskikh

Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: easar@icp.ac.ru
ORCID iD: 0000-0003-1841-0641
SPIN-code: 4183-7660

Dr. Sci. (Biology)

Russian Federation, Chernogolovka, Moscow Region

Elbek A. Machigov

N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences

Email: elbek_machigov@mail.ru
ORCID iD: 0000-0003-2811-6374
SPIN-code: 7382-5408
Russian Federation, Moscow

Andrey I. Yarmolenko

Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences

Email: andr.yar@bk.ru
ORCID iD: 0009-0008-2736-4118
Russian Federation, Chernogolovka, Moscow Region

Elena V. Shtamm

N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences

Email: ekochem@yandex.ru
ORCID iD: 0000-0003-2537-2751

Dr. Sci. (Chemistry)

Russian Federation, Moscow

Serikbai K. Abilev

N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences; Lomonosov Moscow State University

Email: abilev@vigg.ru
ORCID iD: 0000-0001-8636-6828
SPIN-code: 4692-4311
Scopus Author ID: 8723003000

Dr. Sci. (Biology)

Russian Federation, Moscow; Moscow

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Supplementary files

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1. JATS XML
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2. Fig. 1. Chemical formula of N-phosphonomethylglycine

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3. Fig. 4. Scheme of potential reactions of N-PMG oxidation at α-carbon atom

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4. Formula 1

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5. Formula 2

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6. Formula 3

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7. Formula 4

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8. Formula 5

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9. Formula 6

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10. Formula 7

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11. Formula 8

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12. Formula 9

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13. Formula 13

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14. Formula 14

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15. Fig. 2. Change in the absorption spectrum of the reaction mixture during the photochemical decomposition of an aqueous solution of N-phosphonomethylglycine under the combined influence of UV radiation and ozone over time: 1 — absorption intensity at λ = 212 nm; 2 — 195 nm; 3 — 286 nm; 4 — 258 nm; 5 — 238 nm. According to work [41]

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16. Fig. 3. Luminescence of pKatG-lux and pSoxS-lux with N-PMGirr in arbitrary units of luminous flux

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17. Formula 10

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18. Formula 11

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19. Formula 12

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