Analysis of arctic char (Salvelinus alpinus) haplotypes in the largest lakes of the North-West Region of Russia for genetic monitoring
- Authors: Kiseleva M.N.1, Mitryushkina D.K.1, Filatova T.A.1,2, Golotin V.A.1,3, Zhukova A.A.1,4, Rozhkovan K.V.1, Mamaeva A.E.2, Apalikova O.V.1
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Affiliations:
- St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography
- St. Petersburg State University of Veterinary Medicine
- Academician I.P. Pavlov First St. Petersburg State Medical University
- Herzen State Pedagogical University of Russia
- Issue: Vol 21, No 4 (2023)
- Pages: 357-368
- Section: Genetic basis of ecosystems evolution
- URL: https://journals.rcsi.science/ecolgenet/article/view/254604
- DOI: https://doi.org/10.17816/ecogen622885
- ID: 254604
Cite item
Abstract
BACKGROUND: Arctic char is a residential form of the Arctic char Salvelinus alpinus and a commercial target in Ladoga Lake. Caused by fishing the gradual decline in the number of ones in the largest lakes of the North-Western Region of Russia is compensated by measures for its artificial reproduction.
AIM: The aim of this work was to carry out gaplotyping of the arctic char (Salvelinus alpinus) population in the largest lakes of the northwestern region of Russia.
MATERIALS AND METHODS: The gaplotyping by PCR amplification in combination with restriction fragment length polymorphism (PCR-RFLP) of several amplified mtDNA regions, sequencing of the D-loop of mtDNA.
RESULTS: Screening studies of the genetic diversity of arctic char were carried out in three geographical population of the North-Western region of Russia and also the lake form of Arctic char from Sobachye Lake (Putorana Plateau, Western Siberia). The variability of a significant part (more than 35%) of the mitochondrial genome was analyzed including the regions of 16S rRNA/ND1/ND2 (2000 bp), COXI (567 bp), ND5/ND6 (2490 bp) and D-loop (1097 bp). Analysis of the nucleotide sequences of the control region mtDNA revealed 6 different haplotypes that were included in a single Eurasian group of char.
CONCLUSIONS: The panel of PCR-RFLP markers can serve as a tool for monitoring the genetic structure of the arctic char populations of Ladoga Lake under conditions of ones artificial maintenance.
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##article.viewOnOriginalSite##About the authors
Marina N. Kiseleva
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography
Email: marina.marinakisel@yandex.ru
SPIN-code: 5436-3126
Russian Federation, Saint Petersburg
Diana K. Mitryushkina
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography
Email: dianaa.5@yandex.ru
SPIN-code: 4546-5942
Russian Federation, Saint Petersburg
Tatiana A. Filatova
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; St. Petersburg State University of Veterinary Medicine
Email: tanechka23.9292@mail.ru
SPIN-code: 9998-1684
Russian Federation, Saint Petersburg; Saint Petersburg
Vasily A. Golotin
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; Academician I.P. Pavlov First St. Petersburg State Medical University
Author for correspondence.
Email: golotin@bk.ru
ORCID iD: 0000-0003-0385-2463
SPIN-code: 7198-3170
Scopus Author ID: 56641547400
Cand. Sci. (Biology)
Russian Federation, Saint Petersburg; Saint PetersburgAlina A. Zhukova
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; Herzen State Pedagogical University of Russia
Email: gatteriyagreen@gmail.com
ORCID iD: 0000-0002-4416-1901
SPIN-code: 6849-0483
Cand. Sci. (Biology)
Russian Federation, Saint Petersburg; Saint PetersburgKonstantin V. Rozhkovan
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography
Email: 27.tomcat@gmail.com
ORCID iD: 0000-0002-8403-8342
SPIN-code: 2000-6703
Cand. Sci. (Biology)
Russian Federation, Saint PetersburgAnastasia E. Mamaeva
St. Petersburg State University of Veterinary Medicine
Email: anastasiamamaeva43@gmail.com
Russian Federation, Saint Petersburg
Olga V. Apalikova
St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography
Email: olga_apalikova@mail.ru
ORCID iD: 0000-0002-1188-2446
SPIN-code: 6660-4950
Scopus Author ID: 6507160902
Cand. Sci. (Biology)
Russian Federation, Saint PetersburgReferences
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