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Post a Comment The assessment of micronucleus frequency in lymphocytes in the cohort of coal-miners characterized by different polymorphisms of double strand break reparation genes
- Authors: Sinitsky M.Y.1, Volobaev V.P.1, Asanov M.A.1
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Affiliations:
- Kemerovo State University
- Issue: Vol 13, No 4 (2015)
- Pages: 30-33
- Section: Articles
- URL: https://journals.rcsi.science/ecolgenet/article/view/2436
- DOI: https://doi.org/10.17816/ecogen13430-33
- ID: 2436
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Abstract
Background: Coal-miners are exposed to a lot of number of harmful factors (chemical agents, ionizing radiation, heavy metals, coal dust etc.). Material and methods: Venous blood samples extracted from 129 coal-miners. Assessment of cytogenetic damage was performed using the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes. PCR and gel electrophoresis were used to determine polymorphisms in the genes Lig4 (rs1805388) and XRCC4 (rs6869366). Results: We found a significant increase in the frequency of binucleated lymphocytes with micronuclei (MN) and protrusions in carriers of the Ile/ Ile genotype of the Lig4 gene Thr9Ile polymorphism in comparison to Thr/Thr and Thr/Ile genotypes. Conclusions: Thr9Ile polymorphism within Lig4 gene can be used as potential molecular genetic markers of increased individual susceptibility to the complex of harmful factors in coal-mining conditions.
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##article.viewOnOriginalSite##About the authors
Maxim Yur'yevich Sinitsky
Kemerovo State University
Email: max-sinitsky@rambler.ru
PhD student, Biology Faculty, Department of Genetics
Valentin P. Volobaev
Kemerovo State University
Author for correspondence.
Email: volobaev.vp@gmail.com
ORCID iD: 0000-0001-7355-9882
SPIN-code: 2805-5700
Junior Researcher
Russian Federation, 6 Krasnaya str., Kemerovo, 650000Maxim Aydarovich Asanov
Kemerovo State University
Email: asmaks988@gmail.com
Student, Biology Faculty, Department of Genetics
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