Influence of γ-irradiation on the expression of encoding ABA metabolism enzymes in barley embryos

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Abstract

Background. Small doses of radiation stimulate the growth and development of plants including seed germination. ABA plays a key role not only in seed dormancy and germination but also in the regulation of adaptive reactions of plants.

The aim of our work was to study the effect of γ-irradiation of barley seeds in a small doses on the expression of genes encoding ABA biosynthesis enzyme 9-cis-epoxycarotenoid dioxygenase (HvNCED1) and catabolism enzyme ABA 8’-hydroxylase (HvABA8’OH-1).

Materials and Methods. The barley seeds were irradiated at dose range of 4–50 Gy at a dose rate of 60 Gy/h, the radiation source was 60Co. The study was carried out in the embryos within the first 30 hours after germination. Relative gene expression was investigated using real-time PCR (RT-PCR).

Results. It was shown that γ-irradiation of barley seeds changes the expression of ABA biosynthesis and catabolism genes in all experimental groups.

Conclusion. This alterations can lead to a decrease the ABA content under irradiation with stimulating doses and increase the biosynthesis of phytohormone under irradiation with inhibitory dose.

About the authors

Sofia V. Bitarishvili

Russian Institute of Radiology and Agroecology

Author for correspondence.
Email: bitarishvili.s@gmail.com

Jr Researcher, Laboratory of Radiobiology and Ecotoxicology of Plants

Russian Federation, 109 km, Kievskoe shosse, Kaluga region, Obninsk, 249032

Vladimir S. Bondarenko

Russian Institute of Radiology and Agroecology

Email: bvs79@mail.ru

PhD, Leading Researcher, Laboratory for the Study of the Effects of Agrocenoses Exposure to Non-ionizing Radiation

Russian Federation, 109 km, Kievskoe shosse, Kaluga region, Obninsk, 249032

Stanislav A. Geras’kin

Russian Institute of Radiology and Agroecology

Email: stgeraskin@gmail.com

Doctor of Science, Professor, Head of the Laboratory of Radiobiology and Ecotoxicology of Plants

Russian Federation, 109 km, Kievskoe shosse, Kaluga region, Obninsk, 249032

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2. Fig. 1. Transcriptional activity of the gene HvNCED1 encoding the ABA biosynthesis enzyme in embryos depending on the dose of seed irradiation and incubation time in rolls with distilled water. 0 – no detection; *differences are statistically significant compared to control values, p < 0.05, Mann-Whitney U-test

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3. Fig. 2. Transcriptional activity of the gene HvABA8’OH-1 encoding the ABA catabolism enzyme in embryos depending on the dose of seed irradiation and incubation time in rolls with distilled water. * differences are statistically significant compared to control values, p < 0.05, Mann-Whitney U-test

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Copyright (c) 2018 Bitarishvili S.V., Bondarenko V.S., Geras’kin S.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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