A molecular genetic research of the Triticum sinskajae A. Filat. et Kurk. by RAPD analysis and by comparing the nucleotide sequences of the variable intergenic region of the petN-trnC-GCA chloroplast genome and intron of the histone H3.2 gene

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Abstract

Background. Triticum sinskajae A. Filat. et Kurk. was discovered in the early 70th in the last century at the regular reproduction in the Central Asian and Dagestan VIR-stations of T. monococcum samples.

Materials and methods. The objects of the study were 4 species of diploid wheat — Triticum urartu Thum. ex Gandil. (lines k-62477, k-62465), Triticum monococcum L. (lines k-20970, k-39471), Triticum boeoticum Boiss. (lines k-59161, k-28132, k-40118) and Triticum sinskajae A. Filat. et Kurk. (line k-48993).

Results. We found differences between T. sinskajaeand T. monococcum in the variable region of the histone gene H3.2, and the RAPD analysis showed the presence of unique polymorphic loci in T. sinskajae.

Conclusion. In gene ral, T. boeoticum, T. monococcum, and T. sinskajae are most likely to be closely related species of diploid wheat, whereas T. urartu is quite significantly different from them.

About the authors

Azat R. Kuluev

Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics

Author for correspondence.
Email: kuluev.azat91@yandex.ru

Post-graduate Student

Russian Federation, Ufa

Rustam T. Matnijazov

Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics

Email: rmat@mail.ru

Ph.D, Researcher

Russian Federation, Ufa

Bulat R. Kuluev

Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics

Email: kuluev@bk.ru

Doctor of Biology, Senior Researcher

Russian Federation, Ufa

Alexey V. Chemeris

Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics

Email: chemeris@anrb.ru

Doctor of Biology, Professor, Chief Researcher

Russian Federation, Ufa

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2. Fig. 1. A phylogenetic tree constructed from the results of a RAPD-analysis of diploid wheats

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3. Fig. 2. A phylogenetic tree constructed from the analysis of nucleotide sequences of the variable region of the gene encoding H3.2 histone diploid wheat

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4. Fig. 3. A phylogenetic tree constructed by analysis of the nucleotide sequences of the variable intergenic region of petN-trnC-GCA of the chloroplast genome of diploid wheats

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Copyright (c) 2018 Kuluev A.R., Matnijazov R.T., Kuluev B.R., Chemeris A.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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