Moderate thermophilic chemoorganoheterotrophic bacterium in surface layer of anthropogenic grounds of industrial estate area of Al-Mafraq, Jordan

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Abstract

Surface of oil-contaminated soil from Industrial Estate of Al-Mafraq city, Jordan, was investigated for the presence of aerobic oil-degrading moderately thermophilic bacteria. A pure culture of spore – forming aerobic chemoorganogeterotrophic rod shaped bacterial isolate, designated as strain j3n, was obtained. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain j3n is closely related to gram-positive bacteria of kaustophilus – thermoleovorans cluster of Geobacillus genus. Strain j3n grew aerobically with oil, hexadecane, benzoate and acetate. Growth data indicated that utilization of hexadecane but not of oil and benzoate might be under catabolite repression control. Possibility of a regulation of alkane degradation by acetate in aerobic thermophilic gram-positive bacteria of Geobacillus spp. was shown for the first time.

About the authors

Alexander S. Galushko

Agrophysical Research Institute

Email: galushkoas@inbox.ru
ORCID iD: 0000-0002-0387-7997
SPIN-code: 9759-9942
Scopus Author ID: 6603847300
ResearcherId: I-4980-2015

PhD, Cand. Sci. (Med.)

Russian Federation, 14, Grazhdansky av., Saint Petersburg, 195220

Snezanna K. Ibryaeva

Agrophysical Research Institute

Email: snezhanna.ibryaeva@yandex.ru
ORCID iD: 0000-0001-8618-5795

student

Russian Federation, 14, Grazhdansky av., Saint Petersburg, 195220

Anna S. Zhuravleva

Agrophysical Research Institute

Email: zhuravlan@gmail.com
ORCID iD: 0000-0001-7204-9653
SPIN-code: 3084-1394
Scopus Author ID: 57221754069
ResearcherId: I-8764-2018

junior researcher, postgraduate student

Russian Federation, 14, Grazhdansky av., Saint Petersburg, 195220

Gayane G. Panova

Agrophysical Research Institute

Email: gaiane@inbox.ru
ORCID iD: 0000-0002-1132-9915
SPIN-code: 9260-4501
Scopus Author ID: 7003272258
ResearcherId: AAN-6594-2020

PhD, Cand. Sci. (Med.), major researcher

Russian Federation, 14, Grazhdansky av., Saint Petersburg, 195220

Jacob H. Jacob

Al al-Bayt University

Author for correspondence.
Email: jjacob@aabu.edu.jo
ORCID iD: 0000-0003-2410-822X
Scopus Author ID: 36025359900

PhD, Professor

Jordan, P.O. Box 1106 Irbid 21110 - JORDAN

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2. Fig. 1. Phylogenetic position of strain j3n based on the 16S rRNA gene sequences. Clustering was inferred by using the Maximum Likelihood method based on the Tamura–Nei model [30]. The tree with the highest log likelihood is shown. Bar – 0.001 substitutions per nucleotide position

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3. Fig. 2. Growth of strain j3n (shown as change of optical density) by acetate, hexadecane, benzoate and oil utilization. Medium without organic substrates inoculated by bacteria served as control. Axis: Y – OD (optical density of culture at 578 nm), X – time of cultivation (days)

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