Influence of oil pollution on the microbiocenosis of soils adjacent to the oil storage

Cover Page

Cite item

Full Text

Abstract

The aim of the work was an investigation of the effect of hydrocarbons on bacterial and fungal cenoses of sod-podzolic soils on the territory adjacent to the oil storage in the village Malye Kolpany, Leningrad Region. NGS methods were used to analyze the taxonomic composition and structure of the bacteriocenosis and the method of direct microscopy by Demkina-Mirchink to determine the length of the fungal mycelium and the number of spores. Taxonomic and structural changes in the bacterial community led to the dominance of genera containing numerous species-oil destructors. It is established that the main role in the decomposition of oil in the investigated territorybelongs to prokaryotes related to the genera Pseudoxanthomonas, Methylobacterium and Nocardioides. Mycocenosis showed high sensitivity and low adaptability to oil contamination.

About the authors

Anna S. Zhuravleva

Saint Petersburg State University

Author for correspondence.
Email: yardgrave@mail.ru
ORCID iD: 0000-0001-7204-9653

Master of Soil Sciences, Saint Petersburg State University; graduate student, engineer, Agrophysical Research Institute

Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

Natal'ya M. Labutova

Saint Petersburg State University

Email: labutovanm@gmail.ru
ORCID iD: 0000-0001-5229-6104

Doctor of Biological Sciences, Saint Petersburg State University; All-Russian Research Institute for Agricultural Microbiology

Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

Evgeniy E. Andronov

Saint Petersburg State University

Email: eeandr@gmail.com
ORCID iD: 0000-0002-5204-262X

head of laboratory, All-Russian Research Institute for Agricultural Microbiology; senior scientist, Saint Petersburg State University; leading scientist, V. Dokuchaev Soil Science Institute.

Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

References

  1. Шименкова А.А., Потапов А.Д. Система геоэкологического мониторинга нефтехранилищ автозаправочных станций // ФГБОУ ВПО «МГСУ». – 2014. – № 3. – С. 212–219. [Shimenkova AA, Potapov AD. Geo-enviromental monitoring system of the oil storages on petrol stations. Vestnik MGSU. 2014;(3):212-219. (In Russ.)]
  2. Ильин Н.П., и др. Наблюдение за самоочищением почв от нефти в средней и южной тайге // Добыча полезных ископаемых и геохимия природных экосистем. – М.: Наука, 1982. – С. 245–258. [Il’in NP, et al. Observation of soil self-purification from oil in the middle and southern taiga. In: Extraction of minerals and geochemistry of natural ecosystems. Moscow: Nauka; 1982. P. 245-258. (In Russ.)]
  3. Куликова И.Ю. Разработка биопрепарата для ликвидации аварийных разливов на море // Защита окружающей среды в нефтегазовом комплексе. – 2008. – № 5. – С. 59–62. [Kulikova IY. Development of a Biopreparation for the Elimination of Emergency Spills at Sea. Zashhita okruzhajushhej sredy v neftegazovom komplekse. 2008;(5):59-62. (In Russ.)]
  4. Шамраев А.В., Шорина Т.С. Влияние нефти и нефтепродуктов на различные компоненты окружающей среды // Вестник Оренбургского гос. ун-та. – 2009. – № 6. – С. 642–645. [Shamraev AV, Shorina TS. Influence of petroleum and petroleum products to the various components of the environment. Bulletin of Orenburg State University. 2009;6(100):642-645. (In Russ.)]
  5. Стабникова Е.В., Селезнева М.В., Рева О.Н., Иванов В.Н. Выбор активного микроорганизма — деструктора углеводородов для очистки нефтезагрязненных почв // Прикладная биохимия и микробиология. – 1995. – Т. 31. – № 5. – С. 534–539. [Stabnikova EV, Selezneva MV, Reva ON, et al. Vybor aktivnogo mikroorganizma-destruktora uglevodorodov dlya ochistki neftezagryaznennyh pochv. Prikladnajal Biokhimija i mikrobiologija. 1995;31(5):534-539. (In Russ.)]
  6. Околелова А.А., Карасева А.С., Куницына И.А. Методы определения и расчета органических поллютантов в нефтезагрязненных почвах // Фундаментальные исследования. – 2011. – № 8–3. – С. 687–689. [Okolelova AA, Karaseva AS, Kunicyna IA. Metody opredeleniya I rascheta organicheskih pollyutantov v neftezagryaznennyh pochvah. Fundamental’nye issledovaniya. 2011;(8-3):687-689. (In Russ.)]
  7. Андронов Е.Е., и др. Научно-методические рекомендации по выделению высокоочищенных препаратов ДНК из объектов окружающей среды. – СПб., 2011. – 23 с. [Andronov EE, еt al. Nauchno-metodicheskie rekomendacii po vydeleniju vysokoochishhennyh preparatov DNK iz obektov okruzhajushhej sredy. Saint Petersburg; 2011. 23 p. (In Russ.)]
  8. Bates ST, Berg-Lyons JG, Caporaso WA, et al. Examining the global distribution of dominant archaeal populations in soil. ISME J. 2010;5(5):908-917. doi: 10.1038/ismej.2010.171.
  9. Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nature Methods. 2010;7(5):335-336. doi: 10.1038/nmeth.f.303.
  10. Шитиков В.К., Розенберг Г.С. Оценка биоразнообразия: попытка формального обобщения // Количественные методы экологии и гидробиологии (сборник научных трудов, посвященный памяти А.И. Баканова). – Тольятти: СамНЦ РАН, 2005. – С. 91–129. [Shitikov VK, Rozenberg GS. Ocenka bioraznoobraziya: popytka formal’nogo obobshcheniya. In: Kolichestvennye metody ehkologii i gidrobiologii (sbornik nauchnyh trudov, posvyashchennyj pamyati A.I. Bakanova). Tol’yatti: SamNC RAN; 2005. P. 91-129. (In Russ.)]
  11. Dos Santos HF, et al. Mangrove Bacterial Diversity and the Impact of Oil Contamination Revealed by Pyrosequencing: Bacterial Proxies for Oil Pollution. PLoS One. 2011;6(3):e16943. doi: 10.1371/journal.pone.0016943.
  12. Rehmann K, Hertkorn N, Kettrup AA. Fluoranthene metabolism in Mycobacterium sp. strain KR20: identity of pathway intermediates during degradation and growth. Microbiology. 2001;147(10):2783-2794.
  13. Pineda-Flores G, et al. A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation. 2004;15:145-151.
  14. Киреева Н.А., Водопьянов B.B., Мифтахова А.М. Биологическая активность нефтезагрязненных почв. – Уфа: Гилем, 2001. – 376 с. [Kireeva AN, Vodop’janov VV, Miftahova AM. Biologicheskaja aktivnost’ neftezagrjaznennyh pochv. Moscow: Gilem; 2001. 377 p. (In Russ.)]
  15. Гузев В.С., Левин С.В., Селецкий Г.И., и др. Роль почвенной микробиоты в рекультивации нефтезагрязненных почв // Микроорганизмы и охрана почв. – М.: Изд-во МГУ, 1989. – С. 121–150. [Guzev VS, Levin SV, Seleckij GI, et al. Rol’ pochvennoj mikrobioty v rekul’tivacii neftezagrjaznennyh pochv. In: Mikroorganizmy i ohrana pochv. Moscow: Izd-vo MGU; 1989. P. 121-150. (In Russ.)]
  16. Brakstad OG, Lødeng AGG. Microbial diversity during biodegradation of crude oil in seawater from the North Sea. Microb Ecol. 2005;49(1):94-103. doi: 10.1007/s00248-003-0225-6.
  17. Juck D, et al. Polyphasic microbial community analysis of petroleum hydrocarbon contaminated soils from two northern Canadian communities. FEMS Microbiol Ecol. 2000;33(3):241-249.
  18. Bogan BW, et al. Alkanin digesillinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. Int J Syst Evol Microbiol. 2003;53:1389-1395.
  19. Popp N, Schlomann M, Mau M. Bacterial diversity in the active stage of a bioremediation system for mineral oil hydrocarbon-contaminated soils. Microbiology. 2006;152:3291-3304.
  20. Nopcharoenkul W, Netsakulnee P, Pinyakong O. Diesel oil removal by immobilized Pseudoxanthomonas sp. RN402. Biodegradation. 2013;24(3):387-397. doi: 10.1007/s10532-012-9596-z.
  21. Kumari B, Singh SN, Singh DP. Characterization of two biosurfactant producing strains in crude oil degradation. Process Biochemistry. 2013;47(12):2463-2471.
  22. Кононова В.В., Самсонова А.С., Семочкина Н.Ф. Cурфактантобразующая микрофлора: свойства и практическое использование // Микробные биотехнологии: фундаментальные и прикладные аспекты: Сборник научных трудов ГНУ «Институт микробиологии НАН Беларуси», 2007. [Kononova VV, Samsonova AS, Semochkina NF. Surfaktantobrazujushhaja mikroflora: svojstva i prakticheskoe ispol’zovanie. In: Mikrobnye biotehnologii: fundamental’nye iprikladnye aspekty. Sbornik nauchnyh trudov of Institut Mikrobiologii NAN Belarusi; 2007. (In Russ.)]
  23. Мирчинк Т.Г. Почвенная микология. – М.: Изд-во МГУ, 1988. – 220 с. [Mirchink TG. Pochvennaya mikologiya. Moscow: Izd-vo MGU; 1988. (In Russ.)]

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2017 Zhuravleva A.S., Labutova N.M., Andronov E.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies