Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.

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Abstract

BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored.

MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay.

RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed.

CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.

About the authors

Veronika D. Shcherbinina

Saint Petersburg State University

Email: sherbinina.veronika2014@yandex.ru
ORCID iD: 0000-0002-4813-736X

Graduate student

Russian Federation, Saint Petersburg

Marina V. Petrova

Institute of Higher Nervous Activity and Neurophysiology of RAS

Email: mizopsilofit@inbox.ru
ORCID iD: 0000-0003-4385-7509

PhD, Cand. Sci. (Biol.)

Russian Federation, Moscow

Timofey S. Glinin

Saint Petersburg State University

Email: t.glinin@gmail.com
ORCID iD: 0000-0003-0550-741X
SPIN-code: 2549-2759

PhD, Cand. Sci. (Biol.)

Russian Federation, Saint Petersburg

Eugene V. Daev

Saint Petersburg State University; Pavlov Institute of Physiology of the RAS

Author for correspondence.
Email: mouse_gene@mail.ru
ORCID iD: 0000-0003-2036-6790

PhD, Dr. Sci. (Biol.)

Russian Federation, Saint Petersburg; Saint Petersburg

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Supplementary files

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2. Fig. 1. Degree of DNA damage in testes cells of male CD-1 mice after exposure to two different stressors (Me; Range: Min to Max)

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3. Fig. 2. Exponential distribution of damaged testicular cells accordingly DNA content in comet tails (Y0 e(–KX); 95%CI) of mouse males of control group (H2O) (1), and positive control group (males injected with mitomycin C) (2). Parameters Y0 and F0.5 are specified. Data from the first experiment

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4. Fig. 3. Frequency distribution of testicular cells with different DNA content in comet tails (Y0e(–KX); 95%CI) after 2 hrs exposure of CD-1 mouse males to different stressors. 1 – control; 2 – restraint stress; 3 – 2,5-dimethylpyrazine

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5. Fig. 4. Degree of DNA damage in bone marrow cells of male CBA mice after exposure to two different stressors (Me; Range: Min to Max)

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6. Fig. 5. Frequency distribution of bone marrow cells with different DNA content in comet tails after 2 hrs exposure of CBA mouse males to different stressors. 1 – control; 2 – restraint stress; 3 –2,5-dimethylpyrazine

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