Prevalence rates of Wolbachia endosymbiotic bacterium in natural populations of Ostrinia Nubilalis and Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) in South-Western Russia

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Abstract

Background. Endosymbiotic bacteria of the genus Wolbachia are widespread in arthropods and often cause reproductive abnormalities in lepidopteran insects, including corn borers of the genus Ostrinia. Wolbachia-Ostrinia is a promising model for studies of parasite-host interactions yet parasite prevalence in natural insect host populations remains unknown.

Materials and Methods. Molecular genetic screening and statistical analysis is applied to evaluate prevalence rates of Wolbachia in sympatric populations of two corn borer species. Individual genomic DNA samples were extracted from last instar larvae collected in nature from different forage plants. For each sample of DNA showing positive signal with insect-specific primers the detection is performed using three diagnostic loci of Wolbachia: 16SrRNA, gatB and fbpA.

Results. Wolbachia-positive signal is obtained for 13.5% larvae of Ostrinia nubilalis (N = 141) and 31.9% larvae of Ostrinia scapulalis (N = 138). In different localities the Wolbachia prevalence ranged from 2.9% (N = 34) to 65.8% (N = 38). Significantly higher rates of Wolbachia prevalence in insects from mugwort and hemp (O. scapulalis) as compared to those from corn (O. nubilalis) are revealed in three out of four localities.

Conclusions. Endosymbiotic bacteria of the genus Wolbachia are revealed in natural populations of corn borers for the first time for Eastern Europe. The prevalence rates can be high and this should be taken into consideration when reproductive isolation is examined in population of these hosts as well as establishment of laboratory cultures is performed.

About the authors

Yuri S Tokarev

All-Russian Institute for Plant Protection

Author for correspondence.
Email: jumacro@yahoo.com

Senior researcher, Laboratory of microbiological control

Russian Federation, Pushkin, Saint Petersburg, Russia

Mariya A Yudina

Institute of Cytology and Genetics SB RAS

Email: maryjudina@gmail.com

Engineer, Department of Insect Genetics

Russian Federation, Novosibirsk, Russia

Julia M Malysh

All-Russian Institute for Plant Protection

Email: malyshjm@mail.ru

Researcher, Laboratory of agricultural entomology

Russian Federation, Pushkin, Saint Petersburg, Russia

Roman A Bykov

Institute of Cytology and Genetics SB RAS

Email: bykovra@bionet.nsc.ru

Researcher, Department of insect genetics

Russian Federation, Novosibirsk, Russia

Andrei N Frolov

All-Russian Institute for Plant Protection

Email: cornborer@gmail.com

Head of laboratory, Laboratory of agricultural entomology

Russian Federation, Pushkin, Saint Petersburg, Russia

Inna V Grushevaya

All-Russian Institute for Plant Protection

Email: grushevaya_12@mail.ru

Junior researcher, Laboratory of agricultural entomology

Russian Federation, Pushkin, Saint Petersburg, Russia

Yury Y Ilinsky

Institute of Cytology and Genetics SB RAS

Email: paulee@bionet.nsc.ru

Senior Researcher, Department of Insect Genetics

Russian Federation, Novosibirsk, Russia

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Supplementary files

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2. Fig. 1. Locations of insect samples in Krasnodar Territory (1 – hamlet Slobodka; 2 – town Slavyansk-na-Kubani; 3 – settlement Botanika) and Belgorod Territory (4 – settlement Maiskiy)

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3. Table 1 Wolbachia prevalence rates in local populations of corn borer of the Ostrinia genus
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4. Table 2 A summary of Wolbachia prevalence rates in corn borer Ostrinia from different forage plants
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Copyright (c) 2017 Tokarev Y.S., Yudina M.A., Malysh J.M., Bykov R.A., Frolov A.N., Grushevaya I.V., Ilinsky Y.Y.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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