Genotoxic effects of N-nitrosodimethylamine in somatic and generative cells of mice

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N-Nitrosodimethylamine (NDMA) was shown to have genotoxic properties in acute and subacute studies on laboratory mice. The organ-specificity of the genotoxic effect of NDMA was revealed using the Comet assay. The most sensitive organs to the action of NDMA were kidneys and liver. DNA damage in liver cells of NDMA-treated animals at doses of 4.0 and 8.0 mg/kg, increased compared to control in 6.9 and 12.5 (р < 0.001), and in kidney cells – in 8.1 and 14.2 times (р < 0.001), respectively. NDMA also showed genotoxic activity in the reproductive cells of experimental animals, causing structural disorders of synaptonemal complexes in spermatocyte. In NDMA-treated animals at a dose of 2.0 mg/kg in acute and subacute studies, the level of spermatocytes with damaged synaptonemal complexes increased statistically significantly compared to control in 6.0 and 7.0 (р < 0.05) times, respectively.

About the authors

Anna V. Lovinskaya

Al-Farabi Kazakh National University
 

Author for correspondence.
Email: annalovinska@rambler.ru

Researcher worker, Faculty of Biology and Biotechnology, Laboratory of Mutagenesis Research

 

 

Kazakhstan, 71,  al - Farabi ave., Almaty,  050040

Saule Zh. Kolumbayeva

Al-Farabi Kazakh National University

Email: saule.kolumbayeva@kaznu.kz

Professor, Faculty of Biology and Biotechnology, Department of Molecular Biology and Genetics

 

 

Kazakhstan, 71,  al - Farabi   ave., Almaty,  050040

Oksana L. Kolomiets

Vavilov Institute of General Genetics

Email: olkolomiets@mail.ru

Head of the Laboratory of Cytogenetics

Russian Federation, 3, Gubkin str., Moscow, 119991

Serikbay K. Abilev

Vavilov Institute of General Genetics; Lomonosov Moscow State University

Email: abilev@vigg.ru

Professor, Biologiсal faculty, Department of Genetics. Lomonosov Moscow State University; Deputy Director for Science. N.I. Vavilov Institute of General Genetics

Russian Federation, 3, Gubkin str., Moscow, 119991; 1, Leninskie Gory, 119991

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2. Fig. 1. DNA content in the comet tail in the cells of internal organs of mice intoxicated once with N-nitrosodimethylamine

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Copyright (c) 2017 Lovinskaya A.V., Kolumbayeva S.Z., Kolomiets O.L., Abilev S.K.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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