Analysis of ACE, AGTR1, eNOS, MTHFR, MTRR and APOE genes polymorphisms in the population of North-West of Russia


Cite item

Full Text

Abstract

Estimation of population-specific frequencies of relevant polymorphisms in normal control population remains the major prerequisite of these studies. The polymorphisms of seven genes (ACE, AGTR1, eNOS, MTHFR, MTRR, APOE) associated with some multifactorial diseases (cardiovascular diseases, diabetes) were studied by PCR-RFLP analysis in North-West population of Russia. The group consisted of unrelated individuals of middle age (25-45) (59 men and 58 women). Distribution of relevant polymorphisms frequencies for MTHFR, MTRR, APOE, eNOS and AGTR1 genes was similar in our samples compared to these ones in some and European populations (Spain and Germany). Low frequency of D/D genotype and high frequency of I/I genotype were found in Russian compared to Spain population (D/D - 29,6 and 41%, I/I - 26,1 and 15%, respectively). No significant statistical differences in distribution of ACE polymorphisms were found between Russian and Scotland populations (D/D - 31%, I/I - 20%). This population specific differences for the polymorphisms of some genes and its obvious similarity for the other ones were disclosed

About the authors

Oleg S Glotov

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology , Saint-Petersburg, RF

Email: olglotov@mail.ru 199034, Saint-Petersburg, Mendeleyevskaya line, 3. Russia

Andrey S Glotov

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology , Saint-Petersburg, RF

Email: anglotov@mail.ru 199034, Saint-Petersburg, Mendeleyevskaya line, 3, Russia

Olga A Tarasenko

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology , Saint-Petersburg, RF

Email: olgatar777@mail.ru

Tatyana E Ivashchenko

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology , Saint-Petersburg, RF

Email: iagmail@ott.ru Mendeleyev Line, 3, Saint-Petersburg, 199034, Russia

Vladislav S Baranov

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology , Saint-Petersburg, RF

Email: baranov@vb2475.spb.edu

References

  1. Баранов B.C., Баранова Е.В., Иващепко Т.Э., Асеев М.В. Геном человека и гены «предрасположенности» (Введение в предиктивную медицину). -СПб.: Издательство «Интермедика». -2000.
  2. Баранов В. С. Молекулярная медицина: молекулярная диагностика, превентивная медицина и генная терапия//Молекулярная биология. -2000. -Т. 34, № 4. -С. 684-695.
  3. Определение генетической предрасположенности к наследственным и мультифакториальным заболеваниям. Генетический паспорт//Методические рекомендации. -СПб.: ИКФ «Фолиант», 2001. -48 с.
  4. Степанов В.А. Этногеномика неселения Северной Евразии//Томск: Изд-во «Печатная мануфактура», 2002. -244 с.
  5. Степанов В.А., Пузырев В.П. Анализ аллельных частот семи микросателлитных локусов Y-хромосомы в трех популяциях тувинцев//Генетика. -2000. -Т. 36. -С. 241-248.
  6. Хуснутдинова Э.К., Фатхлисламова Р.И., Хидиятова И.М. и др. Рестрикционно-делеционный полиморфизм V-области митохондриальной ДНК в популяциях народов Волго-Уральского региона//Генетика. -1997. -Т. 33. -С. 996-1000.
  7. Шварц Е.И. Современные возможности молекулярной генетики в клинической практике//Матер, симпозиума «Вопросы фармакотерапии в клинической практике: прошлое, настоящее, будущее». -СПб., 1997. -С. 3-6.
  8. Alvarez R., Alvarez V., Lahoz С.Н., Martinez С, Репа J., Sanchez J.M., Guisasola L.M, Salas-Puig J., Moris G., VidalJ.A., Ribacoba R., Menes ВВ., Uria D., Colo E. Angiotensin converting enzyme and endothelial nitric, oxide synthase DNA polymorphisms and late onset Alzheimer's disease//J. Neurol Neurosurg Psychiatry 1999; Vol. 67:733-736.
  9. Batalla A., Alvarez R., Reguero J.R., Hevia S., Iglesias-Cubero G., Alvarez V., Cortina A., Gonzalez P., Celada M.M., Medina A. and Coto E. Synergistic Effect between Apolipoprotein E and Angiotensinogen Gene Polymorphisms in the Risk for Early Myocardial Infarction//Clinical Chemistry 46, 2000. -Vol. 12:1910-1915.
  10. Garte S. et al. Metabolic Gene Polymorphism Frequencies in Control Populations//Cancer Epidemiology, Biomarkers & Prevention, December 2001. -Vol. 10:1239-1248.
  11. Hobbs C.A., Sherman S.L., Yi P., Hopkins S.E., Torfs СР., Hine R. Jean, Pogribna M., Rozen R., and S. Jill James. Polymorphisms in Genes Involved in Folate Metabolism as Maternal Risk Factors for Down Syndrome//Am. J. Hum. Genet., 2000. -Vol. 67: 623-630.
  12. Nebert D. W., Carvan M.J. Ecogenetics: from Biology to Health//Toxicol. Industr. Health. -1997. -Vol. 13: 163-192.
  13. Pinto X., Vilaseca M.A., Garcia-Giralt N. Ferrer I., Pala M., Meco J.F., Mainou C, Ordova J.M., Grinberg D., Balcells S. and the Baix Llobregat. Homocysteine Study Group Homocysteine and the MTHFR 677C-T allele in premature coronary artery disease. Case control and family studies//European Journal of Clinical Investigation. -2001. -Vol. 31:24-30.
  14. Yoshimura K., Hanaoka Т., Ohnami Sh., Ohnami S., Kohno Т., Liu Y., Yoshida Т., Sakamoto H., Tsugane S. Allele frequencies of single nucleotide polymorphisms (SNPs) in 40 candidate genes for gene-environment studies on cancer: data from population-based Japanese random samples//J. Hum Genet. -2003. -Vol. 48: 654-658.

Copyright (c) 2004 Glotov O.S., Glotov A.S., Tarasenko O.A., Ivashchenko T.E., Baranov V.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies