Unique transcriptome features of pea (Pisum sativum L.) lines with differing responses to beneficial soil microorganisms
- Authors: Afonin A.M.1, Gribchenko E.S.1, Zorin E.A.1, Sulima A.S.1, Romanyuk D.A.1, Zhernakov A.I.1, Shtark O.Y.1, Akhtemova G.A.1, Zhukov V.A.1
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Affiliations:
- All-Russia Research Institute for Agricultural Microbiology
- Issue: Vol 19, No 2 (2021)
- Pages: 131-141
- Section: Genetic basis of ecosystems evolution
- URL: https://journals.rcsi.science/ecolgenet/article/view/54703
- DOI: https://doi.org/10.17816/ecogen54703
- ID: 54703
Cite item
Abstract
BACKGROUND: Garden pea (Pisum sativum L.) possesses the ability to form beneficial symbioses with various soil microorganisms. However, different pea cultivars, genotypes, and lines gain more or less benefit from these interactions, so the trait named “efficiency of interaction with soil microorganisms” (EIBSM) was suggested to describe this phenomenon. The molecular mechanisms underlying the manifestation of the EIBSM trait are not properly studied, and only few works focusing on plant responses to combined microbial preparations have been published to date.
METHODS: Eight pea lines previously described as contrasting in manifestation of the EIBSM trait were grown in pots with soil under combined inoculation with nodule bacteria and arbuscular mycorrhizal fungi, and the transcriptome profiles of the whole root systems of the plants were investigated using 3'MACE RNA sequencing.
RESULTS: The relatedness of the lines inferred from the analysis of transcripts’ SNVs (Single Nucleotide Variants) corresponded to the manifestation of the EIBSM trait: three high-EIBSM lines and three low-EIBSM lines formed two distinct clusters. Thus, the gene expression profiles were compared between these two clusters, which enabled identification of transcriptome signatures characteristic for each group. The lines previously described as high-EIBSM have lower symbiotic activity, and the expression levels of pathogen response genes were elevated compared to the lines with low EIBSM.
CONCLUSION: This result suggests that the mechanism of high interaction efficiency may be connected to stricter host control of symbionts, allowing such plants to expend less on the symbioses.
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##article.viewOnOriginalSite##About the authors
Alexey M. Afonin
All-Russia Research Institute for Agricultural Microbiology
Author for correspondence.
Email: afoninalexeym@gmail.com
ORCID iD: 0000-0002-8530-0226
Research engineer
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Emma S. Gribchenko
All-Russia Research Institute for Agricultural Microbiology
Email: gribemma@gmail.com
ORCID iD: 0000-0002-1538-5527
Technician
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Evgeny A. Zorin
All-Russia Research Institute for Agricultural Microbiology
Email: kjokkjok8@gmail.com
ORCID iD: 0000-0001-5666-3020
Research engineer
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Anton S. Sulima
All-Russia Research Institute for Agricultural Microbiology
Email: asulima@arriam.ru
ORCID iD: 0000-0002-2300-857X
PhD, Cand. Sci. (Biol.)
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Daria A. Romanyuk
All-Russia Research Institute for Agricultural Microbiology
Email: daria-rom@yandex.ru
ORCID iD: 0000-0001-9576-1256
PhD, Cand. Sci. (Biol.)
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Alexander I. Zhernakov
All-Russia Research Institute for Agricultural Microbiology
Email: azhernakov@arriam.ru
ORCID iD: 0000-0001-8961-9317
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608
Oksana Yu. Shtark
All-Russia Research Institute for Agricultural Microbiology
Email: oshtark@arriam.ru
ORCID iD: 0000-0002-3656-4559
PhD, Cand. Sci. (Biol.)
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Gulnar A. Akhtemova
All-Russia Research Institute for Agricultural Microbiology
Email: gakhtemova@arriam.ru
ORCID iD: 0000-0001-7957-3693
PhD, Cand. Sci. (Biol.)
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608Vladimir A. Zhukov
All-Russia Research Institute for Agricultural Microbiology
Email: vzhukov@arriam.ru
ORCID iD: 0000-0002-2411-9191
PhD, Cand. Sci. (Biol.)
Russian Federation, 3, Podbelsky highway, Pushkin, Saint Petersburg, 196608References
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