Helminths infestation of various mitochondrial lines of the sable Martes Zibellina and the pine marten M. MARTES

Cover Page

Cite item

Full Text

Abstract

Background. There is an extensive area of introgressive hybridization of the sable and the pine marten in Western Siberia. MtDNA haplotypes mark different phyletic lines of mustelids and can be used to identify their hybrids. Natural focus of filaroidosis, nematodes pathogenic to martens, is situated in Western Siberia. We hypothesized that groups of mustelids different in origin have different susceptibility to nematode infestation. Materials and methods. Animals were caught according to the hunting license in 2009-2014, in 9 localities of Western Siberia. A total of 166 individuals, including 100 sables, 20 pine martens and 46 their hybrids (kidus) were investigated using the method of dissection of the gastrointestinal tract and lungs. Polymorphism of the mitochondrial cytochrome b gene was studied by the method of RFLP (Restriction Fragment Length Polymorphism). Results. Four species of parasitic nematodes Crenosoma petrovi, Filaroides martis, Capillaria putorii, Strongyloides martis were found in martens in Western Siberia. The pine marten was infected by F. martes and S. martis more than the sable (p ˂ 0.05). Hybrids had intermediate levels of nematode infestation. 9 complex haplotypes of mtDNA were identified. Haplotype Z30 prevailed in the sable, AK29 and Z5 - in the pine marten, Z30 and AK23 - in hybrids. Animals from different lines of mtDNA differed significantly by helminths infestation. Animals with a haplotype Z31, typical of the eastern subspecies of sable, were infected with helminths in 2 times more than the animals from Z5 and Z30 lines, typical of the local populations. Animals with a haplotype AK23 have the lowest rate of infections by helminths. Conclusion. Differences in nematode infestation of animals with different mtDNA haplotypes indicates that different phyletic lines of the sable, the pine marten, and their hybrids have varying degrees of susceptibility to nematode infestations.

About the authors

Oxana N Zhigileva

Tyumen State University

Email: zhigileva@mail.ru
associate professor, Ph.D, Department of Ecology and Genetics

Inna M Uslamina

Tyumen State University

Email: inna_golovacheva@mail.ru
postgraduate, Department of Ecology and Genetics

References

  1. Гашев С.Н., Агешин Н.Н. К вопросу о дифференциации представителей рода Martes Тюменской области // Вестник Тюменского государственного университета. - 2003. - № 2. - С. 15-21. [Gashev SN, Ageshin NN. K voprosu o differentsiatsii predstaviteley roda Martes Tyumenskoy oblasti. Vestnik Tyumenskogo gosudarstvennogo universiteta. 2003;2:15-21. (In Russ).]
  2. Граков Н.Н. Филяроидоз и скрябингилез лесной куницы и их влияние на состояние популяции этого вида // Труды ВНИИ животного сырья и пушнины. - 1962. - Т. 19. - С. 298-314. [Grakov NN. Filaroidos i skrjabingilos lesnoy kunitsi i ikh vlijanie na sostoyanie populatsii etogo vida. Trudy VNII zhivotnogo syrja i pushniny. 1962;19:298-314. (In Russ).]
  3. Козлов Д.П. Определитель гельминтов хищных млекопитающих СССР. - М.: Наука, 1977. - 274 с. [Kozlov DP. Opredelitel’ gel’mintov hishhnyh mlekopitajushhih SSSR. M.: Nauka; 1977. (In Russ).]
  4. Контримавичус В.Л. Гельминтофауна куньих и пути ее формирования. - М.: Наука, 1969. - 386 с. [Kontrimavichus VL. Gel’mintofauna kun’ih i puti ee formirovania. M.: Nauka; 1969. (In Russ).]
  5. Павлинин В.Н. Отношения между близкими видами млекопитающих в районах соприкосновения их ареалов (на примере отношений куницы и соболя на Урале): Автореф. дис. … д-ра биол. наук. - Свердловск, 1964. - 42 с. [Pavlinin VN. Otnoshenia mezhdu blizkimi vidami mlekopitajushih v raionah soprikosnovenia ih arealov (na primere otnoshenij kunitsy i sobolja na Urale [dissertation]. Sverdlovsk; 1964. (In Russ).]
  6. Усламина И.М., Жигилева О.Н. Морфология и экологические особенности соболя Martes zibellina, обитающего на территории Тюменской области // Фундаментальные и прикладные аспекты современной биологии: материалы II Всероссийской молодежной научной конференции, Томск, 24-26 ноября 2015 г. - Томск: Издательский дом Томского государственного университета, 2015. - C. 120-122. [Uslamina IM, Zhigileva ON. Morphologia and ecologicheskie osobennosti sobolja Martes zibellina, obitajusthego na territorii Tyumenskoy oblasti. In: D.S. Vorob’ev, editor. Fundamentalnye i prikladnye aspecty sovremennoi biologii [conference proceeding]. Tomsk; 2015:120-122. (In Russ).]
  7. Abramson NI, Rodchenkova EN, Kostygov AY. Genetic variation and phylogeography of the bank vole (Clethrionomys glareolus, Arvicolinae, Rodentia) in Russia with special reference to the introgression of the mtDNA of a closely related species, red-backed vole (Cl. rutilus). Rus J Genet. 2009;45(5):533-545. doi: 10.1134/S1022795409050044.
  8. Balmysheva NP, Solovenchuk LL. Genetic variation of the mitochondrial DNA gene encoding cytochrome b in the Magadan population of sable Martes zibellina L. Rus J Genet. 1999;35(9):1077-1081.
  9. Davison A, Birks JDS, Brookes RC, et al. Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Mol Ecol. 2001;10(10):2479-2488. doi: 10.1046/j.1365-294X.2001.01381.x.
  10. Koepfli KP, Deere KA, Slater GJ, et al. Multigene phylogeny of the Mustelidae: resolving relationships, tempo and biogeographic history of a mammalian adaptive radiation. BMC Biol. 2008;6(10):1-22. doi: 10.1186/1741-7007-6-10.
  11. Malyarchuk B, Derenko M, Denisova G. A mitogenomic phylogeny and genetic history of sable (Martes zibellina). Gene. 2014;550(1):56-67. doi: 10.1016/j.gene.2014.08.015.
  12. Monakhov VG. The ecological structure of sable populations in the nidus of filaroides invasion. Rus J Ecol. 1999;30(6):420-427.
  13. Nieberding CM, Olivieri I. Parasites: proxies for host genealogy and ecology. Trends in Ecology and Evolution. 2007;22(3):156-165. doi: 10.1016/j.tree.2006.11.012.
  14. Rozhnov VV, Meschersky IG, Pishchulina SL, Simakin LV. Genetic analysis of sable (Martes zibellina) and pine marten (M. martes) populations in sympatric part of distribution area in the Northern Urals. Rus J Genet. 2010;46(4):488-492. doi: 10.1134/S1022795410040150.
  15. Sidorovich V, Anisimova EIE. Comparative analysis or the helminthocenoses of the native semiaquatic mustelids (Lutra lutra, Mustela lutreola) in connection with the width of food spectra. IUCN Otter Spec Group Bull. 1999;16(2):76-78.
  16. Statistica for Windows (Computer program manual). Tulsa, OK: StatSoft, Inc., 1998.
  17. Zhigileva ON, Cheboksarova IB. Nematode infestation of sable and pine marten in Western Siberia. In: Seryodkin IV, Miquelle DG, editors. Diseases and parasites of wildlife in Siberia and the Russian Far East. Vladivostok: Dalnauka; 2012. P. 194-198.
  18. Zhigileva ON, Kashtanov SN, Golovacheva IM. Genetic markers for studying current distribution area and population structure of the sable Martes zibellina L. Achievements in the Life Sciences. 2014 a;8(1):10-15. doi: 10.1016/j.als.2014.06.004.
  19. Zhigileva ON, Politov DV, Golovacheva IM, Petrovicheva SV. Genetic variability of sable Martes zibellina L., pine marten M. martes L. and their hybrides in West Siberia: polymorphism of proteins and DNA. Rus J Genet. 2014b;50(5):508-517. doi: 10.1134/S1022795414050135.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Places of sample collection: 1 – Tobolsky, 2 – Vagaysky, 3 – Uvatsky, 4 – Nefteyugansky, 5 – Isetsky, 6 – Tyumensky, 7 – Yalutorovsky, 8 – Nizhnetavdinsky, 9 – Sovetsky areas of Tyumen Region

Download (357KB)
Indexing metadata
3. Fig. 2. The number of helminths in Martes sp. with different mitochondrial gene Cyt b haplotypes

Download (75KB)
Indexing metadata
4. Table 1. Frequencies of complex haplotypes of mtDNA cytochrome b gene in Martes
Download (16KB)
Indexing metadata
5. Table 2. Indices of nematode infestation of mustelids from different areas
Download (74KB)
Indexing metadata
6. Table 3. Indices of helminths infestation (x ± SE)
Download (77KB)
Indexing metadata
7. Table 4. Average number of helminths in mustelids with different mtDNA haplotypes (x ± SE)
Download (74KB)
Indexing metadata

Copyright (c) 2016 Zhigileva O.N., Uslamina I.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies