Microspatial structure of population gene pool in the land snail Helicopsis striata (Pulmonata, Hygromiidae) in conditions of the Southern Mid-Russian Upland

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Abstract

Background. The formation of the subdivided genetic pattern is key to the successful adaptation of population. Consequently work on the conservation and restoration of protected steppe snail Helicopsis striata (Müller 1774) should be based on the analysis microprotrusions differentiation of population gene pools. Materials and methods. The genetic structure of six populations of H. striata was studied in a variety conditions of the Southern Mid-Russian Upland. In each population collected snails from five locations, located at an equal distance from each other (150-200 m). As the genetic markers used six polymorphic loci of allozymes (polyacrylamide gel electrophoresis) and DNA loci (ISSR-PCR). Results. In general, on the Southern Mid-Russian Upland H. striata is characterized by a different degree of the spatial differentiation population gene pools (for example, according to the ISSR-loci 0,078 ≤ Фst ≤ 0,251). In comparison with the other investigated species of land snail in the study area H. striata is distinguished by the high degree of а differentiation population gene pools. It is shown that populations with a high degree of genetic structure found in the biotopes with different levels of the landscape fragmentation. Conclusion. Based on these results it can be stated that an important role in shaping the populations genetic structure in H. striata is not so much the level of diversity environmental conditions, as the degree of migratory activity individuals, which was the highest in genetically rich populations of the basin Seversky Donets river. At the same time the biology of this snail decrease the speed of its active and passive migration, which probably leads to increased isolation of populations. The highest probability of extinction, in our opinion, are isolated populations of H. striata with low genetic variability.

About the authors

Anton A Sychev

Belgorod national research university

Email: syvuch20@yandex.ru
postgraduate, Department of Ecology, Physiology and Evolutionary Biology

Eduard A Snegin

Belgorod national research university

Email: snegin@bsu.edu.ru
Dr. Biol. Sci., senior lecturer, Department of Ecology, Physiology and Evolutionary Biology

References

  1. Литература
  2. Балашёв И.А., Байдашников А.А. Наземные моллюски (Gastropoda) Винницкой области и их биотопическая приуроченность // Вестник зоологии. - 2012. - Т. 46(1). - С. 19-28. [Balashev IA, Baydashnikov AA. Terrestrial molluscs (Gastropoda) of Vinnytsa region and their confinement to different phytocenoses. Vestnik zoologii. 2012;46(1):19-28. (In Russ.)]
  3. Генофонд и геногеография народонаселения / Под ред. Ю.Г. Рычкова. - СПб.: Наука, 2000. [Gene pool and genogeography of human populations. Ed by Yu.G. Rychkov. Saint Petersburg: Nauka; 2000. (In Russ.)]
  4. Гриценко В.В. Эколого-генетическая организация изменчивости популяций некоторых видов растений и насекомых: автореф. дис. … д-ра биол. наук. - Казань, 2008. [Gritsenko VV. Ecological and genetic organization of the variability in populations of some species plants and insects. [dissertation]. Kazan; 2008. (In Russ.)]
  5. Кайданов Л.З. Генетика популяций. - М.: Высшая школа, 1996. [Kaydanov LZ. Genetics of populations. Moscow: Vysshaya shkola; 1996. (In Russ.)]
  6. Крамаренко С.С. Анализ внутрипопуляционной дифференциации на примере фенетической изменчивости наземного моллюска Brephulopsis bidens (Gastropoda: Pulmonata; Buliminidae) // Еколого-функціональні та фауністичні аспекти дослідження молюсків, їх роль в біоіндикації стану навколишнього середовища. - Житомир: Вид-во ЖДУ ім. І. Франка, 2006. - С. 165-169. [Kramarenko SS. Analiz vnutripopulyatsionnoy differentsiatsii na primere feneticheskoy izmenchivosti nazemnogo mollyuska Brephulopsis bidens (Gastropoda: Pulmonata; Buliminidae). In: Ekologo-funktsіonal’nі ta faunіstichnі aspekti doslіdzhennya molyuskіv, їkh rol’ v bіoіndikatsії stanu navkolishn’ogo seredovishcha. Zhitomir: Vid-vo ZhDU іm. І.Franka, 2006. P. 165-169. (In Russ.)]
  7. Крамаренко С.С. Формирование паттернов пространственно-временной изменчивости наземных моллюсков: мультимасштабный подход: автореф. дис. … д-ра биол. наук. - Киев, 2014. [Kramarenko SS. Formirovanie patternov prostranstvenno-vremennoy izmenchivosti nazemnykh mollyuskov: mul’timasshtabnyy podkhod. [dissertation]. Kiev; 2014. (In Russ.)]
  8. Снегин Э.А. Генетическая структура популяций модельных видов наземных моллюсков в условиях урбанизированного ландшафта на примере Сhondrula tridens Müll. (Gastropoda, Pulmonata) // Экологическая генетика. - 2011а. - Т. IX(2). - С. 54-64. [Snegin EA. The genetic structure of model species populations of terrestrial mollusks in conditions of urbanized landscape using the example of Chondrula tridens Müll (Gastropoda, Pulmonata). Russian Journal of Genetics: Applied Research. 2012;2(2):160-170. (In Russ.)]. doi: 10.1134/s2079059712020128.
  9. Снегин Э.А. Оценка жизнеспособности популяций особо охраняемого вида Cepaea vindobonensis (Mollusca, Gastropoda, Pulmonata) в условиях юга лесостепи Среднерусской возвышенности // Вестник КрасГАУ. - 2011б. - № 11. - С. 142-148. [Snegin EA. Vitality estimation of the Cepaea vindobonensis (Mollusca, Gastropoda, Pulmonata) especially protected species populations in the conditions of the Forest-Steppe in Southern Mid-Russian Upland. Vestnik KrasGAU. 2011b;(11):142-148. (In Russ.)].
  10. Снегин Э.А. Оценка состояния популяционных генофондов наземных моллюсков в условиях влияния горно-обогатительных комбинатов на примере Bradybaena fruticum Müll (Gastropoda, Pulmonata) // Экологическая генетика. - 2010. - Т. VIII(2). - С. 45-55. [Snegin EA. Assessment of the state of population gene pools of terestrial mollusks in conditions of influence of ore dressing combines from the example of Bradybaena fruticum Mull. (Gastropoda, Pullmonata). Russian Journal of Genetics: Applied Research. 2011;1(5):379-389. (In Russ.)]. doi: 10.1134/ s2079059711050133.
  11. Снегин Э.А., Сычев А.А. Оценка жизнеспособности популяций особо охраняемого вида Helicopsis striata Müller (Mollusca, Gastropoda: Pulmonata) в условиях юга Среднерусской возвышенности // Теоретическая и прикладная экология. - 2011. - № 2. - С. 83-92. [Snegin EA, Sychev AA. Estimation of population viability specially protected species Helicopsis striata Müller (Mollusca, Gastropoda, Pulmonata) in Southern Mid-Russian Upland. Theoretical and Applied Ecology. 2011;(2):83-92. (In Russ.)]
  12. Снегин Э.А. Оценка состояния популяционных генофондов особо охраняемого вида Helicopsis striata (Mollusca, Gastropoda, Pulmonata) на основе ДНК-маркеров // Экологическая генетика. - 2015. - Т. XIII(3). - С. 28-39. [Snegin EA. Assessment of the state population gene pool of specially protected species Helicopsis striata (Mollusca, Gastropoda, Pulmonata) using DNA-markers. Ekologicheskaya genetika. 2015; XIII(3):28-39. (In Russ.)]. doi: 10.17816/ecogen13328-39.
  13. Снегин Э.А. Пространственные и временные аспекты эколого-генетической структуры популяций беспозвоночных животных (на примере наземных моллюсков и насекомых юга Среднерусской возвышенности): автореф. дис. … д-ра биол. наук. - Белгород, 2012. [Snegin EA. Extensional and temporal aspects of ecological and genetic structure of invertebrates populations (for example, land snails and insects of the Southern Mid-Russian Upland). [dissertation]. Belgorod; 2012. (In Russ.)]
  14. Снегин Э.А. Улитка степная ребристая (Helicopsis striata Müll) // Красная книга Белгородской области. Редкие и исчезающие растения, грибы, лишайники и животные. Официальное издание / Под ред. А.В. Присного. - Белгород: Белгородская областная типография, 2004. [Snegin EA. Ulitka stepnaya rebristaya (Helicopsis striata Müll). In: Krasnaya kniga Belgorodskoy oblasti. Redkie i ischezayushchie rasteniya, griby, lishayniki i zhivotnye. Ofitsial’noe izdanie. Ed by A.V. Prisnogo. Belgorod: Belgorodskaya oblastnaya tipografiya; 2004. (In Russ.)]
  15. Шварц С.С. Экологические закономерности эволюции. - М.: Наука, 1980. [Shvarts SS. Environmental laws of evolution. Moscow: Nauka; 1980. (In Russ.)]
  16. Шилейко А.А. Наземные моллюски надсемейства Helicoidea. Фауна СССР. Моллюски. - Л.: Наука, 1978. - Т 3(6). - 384 с. [Shileiko AA. Terrestrial molluscs superfamily Helicoidea. Fauna USSR. Molluscs. Leningrad: Nayka; 1978;3(6). (In Russ.)]
  17. Arnaud JF, Madec L, Bellido A, Guiller A. Microspatial genetic structure in the land snail Helix aspersa (Gastropoda: Helicidae). Heredity. 1999;83: 110-119.
  18. Arnaud JF, Madec L, Guiller A, Bellido A. Spatial analysis of allozyme and microsatellite DNA polymorphisms in the land snail Helix aspersa (Gastropoda: Helicidae). Molecular Ecology. 2001;10:1563-1576. doi: 10.1046/ j.1365-294x.2001.01292.x.
  19. Goudet J. F-stat Software ver. 2.9.3.2. Institute of Ecology, Lausanne, Switzerland. 2002.
  20. Livshits GM. Survival, behaviour and spatial distribution of shell morphs in a population of the snail Brephulopsis bidens (Pulmonata). Oecologia (Berl). 1981;51:220-226.
  21. Mathew CG. The isolation of high molecular weight eukaryotic DNA. Nucleic Acids. 1984;2:31-34.
  22. Peakall R., Smouse PE. GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 2006;6:288-295.
  23. Pfenninger M. Relationship between microspatial population genetic structure and habitat heterogeneity in Pomatias elegans (O.F. Müller, 1774) (Caenogastropoda, Pomatiasidae). Biological J of the Linnean Society. 2002;76:565-575.
  24. Pfenninger M, Bahl A, Streit B. Isolation by distance in a population of a small land snail Trochoidea geyeri: Evidence from direct and indirect methods. Proceedings of the Royal Society of London B. 1996;263(1374):1211-1217.
  25. Selander RK, Kaufman DW. Genetic structure of populations of the brown snail (Helix aspersa). I. Microgeographic variation. Evolution. 1975;29:385-401.
  26. Wright S. The genetical structure of populations. Ann Eugen. 1951;15:323-354.

Supplementary files

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2. Fig. 1. The location of the studied H. striata populations in the South of Mid-Russian Upland: 1 – “White mountain”; 2 – “Belenihino”; 3 – “Gubkin”; 4 – “Zasosna”; 5 – “High Lubjanki”; 6 – “Osadche”

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3. Fig. 2. Elektoroforegramm demonstrating the presence of 0-alleles at the locus EST5 (a) population “Zasosna” and the locus EST6 population “White mountain” (b). Indicate the genotypes of individuals at these loci

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4. Fig. 3. The graphic image of the investigated allozyme loci and their associated combinations of alleles

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5. Fig. 4. Debec polygons, built on alleles frequencies of allozymes and ISSR loci in the dem (1-5) of H. striata populations

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6. Fig. 5. Values of allelic frequencies of allozyme loci in the dem population is “White mountain”

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Copyright (c) 2016 Sychev A.A., Snegin E.A.

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