Influence of phytoecdysteroids and plants steroidal glycosides on the lifespan and stress resistance of drosophila melanogaster

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Abstract

Background. Elucidation of the molecular mechanisms of effects of the active substances of plant adaptogens is a topical area of researches. Materials and methods. We studied the effect of herbal substances containing phytoecdysteroids (20-hydroxyecdysone and inokosterone) of Serratula coronata L. or steroidal glycosides (dioscin and protodioscine) of Trigonella foenum-graecum L. on the expression level of stress response genes (genes of heat shock proteins, DNA repair, antioxidant defense and apoptosis), stressresistanse (paraquat, starvation, hyperthermia) and lifespan of Drosophila melanogaster. Results. The studied herbal substances upregulated genes of antioxidant defense mechanisms (Sod1), but downregulated the DNA repair (XPF and Rad51) and apoptosis (Hid) genes. At the same time herbal substances induced weak adaptogenic and antiaging effects. Conclusion. Our results demonstrate that the herbal substances containing phytoecdysteroids and steroidal glycosides change the expression level of stress-response genes and activate mechanisms of hormesis.

About the authors

Mikhail Vyacheslavovich Shaposhnikov

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: mshaposhnikov@mail.ru
Leading Researcher, PhD, Associate Professor, Laboratory of Molecular Radiobiology and Gerontology

Lyubov' Alekseevna Shilova

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: lyubov.schilova@yandex.ru
assistant, Laboratory of Molecular Radiobiology and Gerontology

Ekaterina Nikolaevna Plyusnina

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: kateplus@mail.ru
Researcher, PhD, Laboratory of Molecular Radiobiology and Gerontology

Svetlana Olegovna Volodina

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: volodina@ib.komisc.ru
Head of Laboratory, Doctor of Biological Sciences, Professor, Laboratory of Biotechnology

Vladimir Vital'yevich Volodin

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: volodin@presidium.komisc.ru
Head of Laboratory, Doctor of Biological Sciences, Professor, Laboratory of Biotechnology

Aleksey Aleksandrovich Moskalev

Institute of Biology of the Komi Science Centre of the Ural Division RAS

Email: amoskalev@list.ru
Head. Laboratory, Doctor of Biological Sciences, Associate Professor, Laboratory of Molecular Radiobiology and Gerontology

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Copyright (c) 2014 Shaposhnikov M.V., Shilova L.A., Plyusnina E.N., Volodina S.O., Volodin V.V., Moskalev A.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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