Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae

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Abstract

The development of methods of genetic engineering of plants poses in modern society the question of the safety of transgenic plants. Transformation of plants has been criticized as artificial process performed only in the laboratory. At the same time there is evidence that some members of the genus Nicotiana and Linaria contain sequences homologous to the T-DNA of Agrobacterium rhizogenes in their genomes. The question: whether there are other examples of horizontal gene transfer from Agrobacterium to the plant among the representatives of the family. Solanaceae, is still actual. We performed screening of representatives of four genera of the family Solanaceae for the presence of sequences homologous to oncogeniclike genes rolB, rolC, ORF13, ORF14. New examples of horizontal gene transfer were not detected. Apparently, the presence of sequences homologous to oncogenes of A. rhizogenes in the genome of nontransformed plants is not common to the whole family Solanaceae and is characteristic only of the some species of the genus Nicotiana.

About the authors

Olga Alekseyevna Kulayeva

All-Russia Research Institute for Agricultural Microbiology

Email: koa1983@yandex.ru
Candidate of Biological Sciences, Laboratory of molecular and cellular biology

Tatiana Valeryevna Matveeva

Saint-Petersburg State University

Email: radishlet@gmail.com
Candidate of Biological Sciences, Senior Researcher

Lyudmila Alekseyevna Lutova

Saint-Petersburg State University

Email: la.lutova@gmail.com
Doctor of Biological Sciences, Professor. Deptartament of Genetics and Biotechnology

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Copyright (c) 2013 Kulayeva O.A., Matveeva T.V., Lutova L.A.

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