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Post a Comment Cytogenetic status in coal-miners with occupational pulmonary diseases and influence the polymorphisms of XpD and XpG genes
- Authors: Volobaev V.P.1, Sinitskiy M.Y.1, Kulemin Y.E.1
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Affiliations:
- Kemerovo State University
- Issue: Vol 13, No 4 (2015)
- Pages: 12-15
- Section: Articles
- URL: https://journals.rcsi.science/ecolgenet/article/view/2431
- DOI: https://doi.org/10.17816/ecogen13412-15
- ID: 2431
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Abstract
Background. Coal-miners are exposed by various harmful factors (chemical agents, ionizing radiation, heavy metals, coal dust). Occupational pulmonary diseases may affect to the level of genotoxic damages. Materials and methods. Venous blood samples extracted from 90 coal-miners with various occupational pulmonary diseases and 26 healthy coal-miners (control 1). Blood samples obtained from 124 non-exposed men were used as the control 2. Assessment of cytogenetic damages was performed usingthe analysis of chromosome aberrations. PCR and gel electrophoresis were used to determine polymorphismsin the genes XpD(rs13181) and XpG(rs17655). Results. We found a significant increase in the frequency of the chromatid- and chromosome-type aberrations in coal-miners and the increase of the chromosomal interchange in miners with occupational pulmonary diseases. The modify effects of polymorphismsin the XpD and XpG genes to the level of chromosome aberrations were discovered. Conclusions. It was shown that the XpD and XpG genes can be used as potential molecular geneticmarkers of increased individual risk of occupational pulmonary diseases.
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##article.viewOnOriginalSite##About the authors
Valentin P. Volobaev
Kemerovo State University
Author for correspondence.
Email: volobaev.vp@gmail.com
ORCID iD: 0000-0001-7355-9882
SPIN-code: 2805-5700
Junior Researcher
Russian Federation, 6 Krasnaya str., Kemerovo, 650000Maksim Yur'yevich Sinitskiy
Kemerovo State University
Email: max-sinitsky@rambler.ru
PhD student, biological faculty, departament of Genetics
Yuriy Evgen'yevich Kulemin
Kemerovo State University
Email: jura.kulemin@gmail.com
PhD student, biological faculty, departament of Genetics
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