Identification of complementary genes of hybrid lethality in crosses of bread wheat and rye. Results and perspectives

Cover Page

Cite item

Full Text

Abstract

Numerous genes were found in crop plants, leading to death or sterility of distant hybrids. However, the evolutionary role of concrete genes in the reproductive isolation of the species remains unclear. The article summarizes the own data for identification of hybrid lethality genes that were detected in wheat -rye crosses. Seeds with the dead, undifferentiated embryo and normal endosperm were found in crosses of bread wheat with four out of a hundred studied inbred lines of rye. It is shown that the hybrid lethality is a consequence of the negative complementary interaction of genes of wheat and rye. Rye gene Eml-1R is represented by two alleles - dominant incompatible and recessive compatible. It was mapped on chromosome 6R relative microsatellite and isozyme loci. Complementary wheat gene Eml-1A is located on chromosome 6A. Location of interacting genes on syntenic fragments of chromosomes 6R and 6A may indicate gomeology of hybrid lethality genes in wheat and rye. However, embryonic lethality observed in individual wheat-rye hybrids, cannot be attributed to the evolutionary developed intergeneric incompatibility, like a well-studied prezygotic incompatibility of wheat and related genera.

About the authors

Natalia Dmitrievna Tikhenko

St Petersburg Branch Russian Academy of Sciences, Vavilov Institute of General Genetics

Email: tikhenko@mail.ru
senior researcher, Dr. Biol. Sci., Laboratory of Genetics and Plant Biotechnology

Natalia Vladimirovna Tsvetkova

Saint Petersburg State University

Email: ntsvetkova@mai.ru
research scientist, PhD, Department of Genetics and Biotechnology

Anna Nikolaevna Lyholay

Saint Petersburg State University

Email: lankira@mail.ru
Junior Researcher, Department of Genetics and Biotechnology

Anatoly Vasilievich Voylokov

St Petersburg Branch Russian Academy of Sciences, Vavilov Institute of General Genetics

Email: av_voylokov@mail.ru
Head of the Laboratory, Dr.Biol.Sci., Laboratory of Genetics and Plant Biotechnology

References

  1. Войлоков А. В. (2008) Генетическое картирование у ржи Secale cereale L. Автореф. дис… докт. биол.наук. СПб.
  2. Войлоков А. В., Тихенко Н. Д. (2009) Генетика постзиготической изоляции у растений. Генетика. Т. 45 (6): С. 729-744.
  3. ГОСТ 12039-82 (2011). Межгосударственный стандарт. Семена сельскохозяйственных культур. Методы определения жизнеспособности. Москва: Стандартинформ.
  4. Ригин Б. В., Орлова И. Н. (1977) Пшенично-ржаные гибриды. Ленинград: Колос.
  5. Тихенко Н. Д. (2011) Генетика пшенично-ржаных гибридов и первичных октоплоидных тритикале Автореф. дис… докт. биол. наук. СПб.
  6. Тихенко Н. Д., Цветкова Н. В., Войлоков А. В. (2005) Генетический контроль эмбриональной летальности при скрещивании мягкой пшеницы с рожью. Генетика. T. 41 (8): С. 1075-1083.
  7. Alfares W., Bouguennec A., Balfourier F. et al. (2009) Fine mapping and markers development for the crossability gene SKr on chromosome 5BS of hexaploid wheat (Triticum aestivum L.) Genetics. V. 183: P. 469-481.
  8. Balatero C. H., Darvey N. L. (1993) Influence of selected wheat and rye genotypes on the direct synthesis of hexaploid triticale. Euphytica. V. 66: P. 179-185.
  9. Deng-Cai L., Chi Y., Jun-Liang Y. et al. (1999) The chromosomal locations of high crossability genes in tetraploid wheat Triticum turgidum L. cv. Ailanmai native to Sichuan, China. Euphytica. V. 108: P. 79-82.
  10. Devos K. M., Atkinson M. D., Chinoy C. N. et al. (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor. Appl. Genet. V. 85: P. 673-680.
  11. Dobzhansky T. (1937) Genetics and the Origin of Species. N. Y.: Columbia Univ. Press.
  12. Halloran G. M. (1981) Tetraploid wheat crossability with rye (Secale). Genetica. V. 55: P. 191-194.
  13. Ganal M. W., Röder M. S. (2007) Microsatellite and SNP markers in wheat breeding. In Genomics Assisted Crop Improvement. V. 2: Genomics Applications in Crops, p. 1-24.
  14. Khlestkina E. K., Than M. H.M, Pestsova E. G. et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. V. 109: P. 725-732.
  15. Kosellek С., Pillen K., Nelson J. C. et al. (2013) Inheritance of field resistance to Septoria tritici blotch in the wheat doubled-haploid population Solitär x Mazurka. Euphytica. V. 194: P. 161-176.
  16. Lange W., Wojciechowska B. (1976) The crossing of common wheat (Triticum aestivum L.) with cultivated rye (Secale cereale L.). I. Crossability, pollen grain germination and pollen tube growth. Euphytica. V. 25: P. 609-620.
  17. Lein A. (1943) The genetical basis of the crossability between wheat and rye. Z. Indukt. Abstamm. Vererbungsl. V. 81: P. 28-59.
  18. Marais G. F., Pienaar R. de V. (1977) Hybridisation between wheat and rye. II. Variations in the germinability of the hybrid kernels with special reference to the effect of the D-genome. Agroplantae. V. 9: P. 143-148.
  19. Martis M. M., Zhou R., Haseneyer G. et al. (2013) Reticulate evolution of the rye genome. The Plant Cell. V. 25: P. 3685-3698.
  20. Muller H. J. (1942) Isolating mechanisms, evolution and temperature. Biol. Symp. V. 6: P. 71-125.
  21. Orr H. A. (1996) Dobzhansky, Bateson and the genetics of speciation. Genetics. V. 144: P. 1331-1335.
  22. Pilch J. (2001) Crossability effects of spring wheat (Triticum durum Desf.) with rye (Secale cereale L.) genotypes. Plant breeding and seed science. V. 45 (2): P. 33-43.
  23. Rieseberg L. H., Blackman B. K. (2010) Speciation genes in plants. Annals of Botany. V. 106: P. 439-455.
  24. Riley R., Chapman V. (1967) The inheritance in wheat of crossability with rye. Genet. Res. V. 9: P. 259-267.
  25. Scoles G. J. (1983) The effect of rye genotype on wheat - rye crossability and on the development of F1 seed. Can. J. Genet. Cytol. V. 25: P.668-670.
  26. Sitch L. A., Snape J. W., Firman S. J. (1985) Intrachromosomal mapping of crossability genes in wheat (Triticum aestivum). Theor. Appl. Genet. V. 70: P. 309-314.
  27. Thomas J. B., Kaltsikes P. J., Anderson G. (1980) Relation between whet-rye crossability and seed set of common whet after pollination with other species in the Hordeae. Euphytica. V. 30: P. 121-127.
  28. Tikhenko N., N. Tsvetkova, S. Priyatkina et al. (2011) Gene mutations in rye causing embryo lethality in hybrids with wheat - allelism test and chromosomal localization. Biologia Plantarum. V. l, 55 (3): P. 448-452.
  29. Tikhenko N., Tsvetkova N., Voylokov A. (2010) Embryo lethality in wheat x rye hybrids - mode of inheritance and the identification of a complementary gene in wheat. Euphytica. V. 176: P. 191-198.
  30. Voylokov A. V., Tikhenko N. D. (2002) Triticale as a model for study of genome interaction and genome evolution in allopolyploid plants. Proc. 5th Intern. Triticale Symp. Radzikow, 30 June-5 July 2002. V. 1. P. 63-69
  31. Zheng Y.L, Luo M. C., Yen C., Yang J. L. (1992) Chromosome location of a new crossability gene in common wheat. Wheat Inf. Serv. V. 75: P. 36-40.

Copyright (c) 2015 Tikhenko N.D., Tsvetkova N.V., Lyholay A.N., Voylokov A.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies