Wolbachia Infection among Fleas (Siphonaptera: Insecta) of Sverdlovsk Region and Khabarovsk Territory

Cover Page

Cite item

Full Text

Abstract

Wolbachia are maternally inherited symbiotic bacteria that are widespread among arthropods and filarial nematode parasites. It has been found in all main insect orders although some of them are still insufficiently studied. We performed PCR-screening for 15 species, 252 samples of fleas (Siphonaptera), that had been collected in the Sverdlovsk region and Khabarovsk territory. Six new symbiotic associations of Wolbachia-Siphonaptera were found for the first time. On the basis of earlier published data and the results of the current study we estimate that a minimum of 500 out of 2000 species in the Siphonaptera order are infected with Wolbachia

About the authors

Yury Yuryevich Ilinsky

Institute of Cytology and Genetics

Email: paulee@bionet.nsc.ru
Candidate of Biological Sciences, Laboratory of Populations Genetics, Novosibirsk State University, Department of Cytology and Genetics

Mariya Aleksandrovna Yudina

Novosibirsk State University

Email: maryjudina@gmail.com
Student, Department of Cytology and Genetics

Ekaterina Alekseevna Kalmykova

Novosibirsk State University

Email: ekkalm@yandex.ru
Student, Department of Cytology and Genetics

Roman Andreyevich Bykov

Institute of Cytology and Genetics

Email: bikovra@gmail.com
Postgraduate student, Laboratory of Populations Genetics

Nelya Pavlovna Vysochina

Khabarovsk Antiplague Station

Email: neljavis@mail.ru
Head of the laboratory

Natalya Petrovna Vinarskaya

Omsk State Research Institute of Natural Foci Infections

Email: vinarskayan@inbox.ru
Can. of Biological Sciences, Lab. of Arthropod-Borne Viral Infections

Ilya Kuzmich Zakharov

Institute of Cytology and Genetics

Email: zakharov@bionet.nsc.ru
Doctor of Biological Sciences, Professor Head of the laboratory of Populations Genetics, Department of Cytology and Genetics

References

  1. Медведев С. Г., 1990. Классификация блох и проблемы ее обоснования // Успехи медицинской энтомологии и акарологии в СССР. С. 20–22.
  2. Медведев С. Г., 1998. Классификация отряда блох (Siphonaptera) и ее теоретические предпосылки //Энтомологическое обозрение. T. 77. C. 916–934.
  3. Медведев С. Г., 1994. Морфологические основы классификации отряда блох (Siphonaptera): Автореф. дис... докт. биол. наук. СПб, 50 c.
  4. Медведев С. Г., 2009. Систематика и географическое распространение и пути эволюции блох // Труды зоологического института РАН. Т. 313. C. 273–282.
  5. Росс Г., Росс Ч., Росс Д., 1985. Энтомология. М.: Мир. 576 с.
  6. Baldo L., Dunning Hotopp J. C., Jolley K. A., et al., 2006. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis // Applied Environmental Microbiology. Vol. 72. P. 7098–7110.
  7. Dittmar K., Whiting M. F., 2004. New Wolbachia endosymbionts from nearctic and neotropical fleas (Siphonaptera) // J. Parasitol. Vol. 90. P. 953–957.
  8. Fischer P., Schmetz C., Bandi C. et al., 2002. Tunga penetrans: molecular identification of Wolbachia endobacteria and their recognition by antibodies against proteins of endobacteria from filarial parasites // Experimental Parasitology. Vol. 102. P. 201–211.
  9. Gorham C. H., Fang Q. Q., Durden L. A., 2003. Wolbachia endosymbionts in fleas (Siphonaptera) // J. Parasitol. Vol. 89. P. 283–289.
  10. Hilgenboecker K., Hammerstein P., Schlattmann P. et al., 2008. How many species are infected with Wolbachia? — A statistical analysis of current data //FEMS Microbiol. Lett. Vol. 281. P. 215–220.
  11. Jeyaprakash A., Hoy M. A., 2000. Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76 % of sixty-three arthropod species // Insect Molecular Biology. Vol. 9, N 4. P. 393–405.
  12. Luchetti A., Mantovani B., Fioravanti M. L., Trentini M., 2004. Wolbachia infection in the newly described Ecuadorian sand flea, Tunga trimamillata // Exp. Parasitol. Vol. 108. P. 18–23.
  13. Luchetti A., Mantovani B., Trentini M., 2005. Wolbachia superinfection in an Ecuadorian sample of the sand-flea Tunga penetrans (Preliminary note) // Bull. Ins. Vol. 58. P. 93–94.
  14. Pampiglione S, Fioravanti M. L., Gustinelli A. et al., 2009. Sand flea (Tunga spp.) infections in humans and domestic animals: state of the art // Medical and Veterinary Entomology. Vol. 23. P. 172–186.
  15. Rolain J-M., Franc M., Davoust B., Raoult D., 2003. Molecular detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis and Wolbachia pipientis in cat fleas, France // Emerging Infectious Diseases. Vol. 9. P. 338–342.
  16. Ros V. I. D., Fleming V. M., 2009. How diverse is the Genus Wolbachia? Multiple-gene sequencing reveals a putatively new Wolbachia supergroup recovered from spider mite (Acari: Tetranychidae) // Applied and Environment Microbiology. Vol. 7. P. 1036–43.
  17. Werren J. H., 1997. Biology of Wolbachia // Annual Review of Entomology. Vol. 42. P. 587–609.
  18. Whiting M. F., Whiting A. S., Hastriter M. W. et al., 2008. A molecular phylogeny of fleas (Insecta: Siphonaptera) and host association // Cladistics. Vol. 24. P. 677–707.
  19. Zag P., Hammerstein P., 2012. Still a host of hosts for Wolbachia: analysis of recent data suggests that 40% of terrestrial arthropod species are infected // PloS ONE. Vol. 7. I. 6. e38544.

Copyright (c) 2013 Ilinsky Y.Y., Yudina M.A., Kalmykova E.A., Bykov R.A., Vysochina N.P., Vinarskaya N.P., Zakharov I.K.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies