Culturable endophytic bacteria from stems and leaves of garden pea (Pisum sativum L.)

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Abstract

Background. Endophytic microorganisms inhabit internal tissues of most plants. However, little is known about endophytic community of the garden pea (Pisum sativum L.), an agriculturally important crop.

Materials and methods. Culturable endophytic bacteria were isolated from sterilized stems and leaves of three pea genotypes: K-8274 (cv. Vendevil), K-3358 (unnamed cultivar), and cv. Triumph. The taxonomic position of isolates was determined by 16S rRNA gene sequencing. The plant growth-promoting capabilityof identified bacteria was tested on the roots of watercress (Lepidium sativum L.).

Results. In total, out of 118 morphotypes of culturable endophytic bacteria identified, for 80 the taxonomic position was determined. Proteobacteria and Firmicutes were dominant phyla, and Actinobacteria were present in minority. Eight bacterial isolates demonstrated the plant growth-promoting capability, and one of them – KV17 (Rahnella sp.) maintained this capability after several passages and prolonged storage.

Conclusion. The plant growth-promoting bacteria isolated from pea stems and leaves can become a component of microbiological preparations.

About the authors

Ekaterina N. Vasileva

All-Russia Research Institute for Agricultural Microbiology

Author for correspondence.
Email: evasilieva@arriam.ru
ORCID iD: 0000-0001-5599-0361

Technician, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, Saint-Petersburg

Gulnar A. Akhtemova

All-Russia Research Institute for Agricultural Microbiology

Email: ahgulya@yandex.ru
ORCID iD: 0000-0001-7957-3693

PhD, Senior Scientist, Laboratory of Genetics of Plant-Microbe Interactions.

Russian Federation, Saint-Petersburg

Alexey M. Afonin

All-Russia Research Institute for Agricultural Microbiology

Email: afoninalexeym@gmail.com
ORCID iD: 0000-0002-8530-0226

PhD student, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, Saint-Petersburg

Alexey Yu. Borisov

All-Russia Research Institute for Agricultural Microbiology

Email: ayborisov@yandex.ru
ORCID iD: 0000-0001-9834-7368

DSc, Chief Researcher

Russian Federation, Saint-Petersburg

Igor A. Tikhonovich

All-Russia Research Institute for Agricultural Microbiology; Saint Petersburg State University

Email: arriam2008@yandex.ru
ORCID iD: 0000-0001-8968-854X

DSc, Scientific Director; Professor, Dean, Faculty of Biology

Russian Federation, Saint-Petersburg

Vladimir A. Zhukov

All-Russia Research Institute for Agricultural Microbiology

Email: vzhukov@arriam.ru
ORCID iD: 0000-0002-2411-9191

PhD, Head, Laboratory of Genetics of Plant-Microbe Interactions

Russian Federation, Saint-Petersburg

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Supplementary files

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2. Fig. 1. Morphotypes of endophytic bacteria isolated from the internal tissues of stems and leaves of garden pea

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3. Fig. 2. Representation of phyla of endophytic bacteria isolated from stems and leaves of various pea genotypes. (In case of leafless pea sort “Triumph” stipules were studied instead of leaves)

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4. Fig. 3. Members of the endophytic community of pea plants. The diagrams show the number of representatives of various genera of bacteria

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5. Fig. 4. The length of the watercress root when inoculated with endophytic bacteria isolated from stems and leaves (stipules). Experiments are marked by numbers (1–5). For each experiment an individual control was set. The p-values for differences between root length of tested plant and control plant are marked as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns – no significant differences

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6. Fig. 5. Results of a repeated plant growth-promotion test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns – no significant differences

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7. Results of growth-stimulating activity tests for endophytic bacteria. Significance of difference in the length of plant roots from control: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; ns – no significant differences

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8. Results of growth-stimulating activity tests for endophytic bacteria. Significance of difference in the length of plant roots from control: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; ns – no significant differences

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Copyright (c) 2020 Vasileva E.N., Akhtemova G.A., Afonin A.M., Borisov A.Y., Tikhonovich I.A., Zhukov V.A.

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