Differential distribution of 5-formylcytosine and 5-carboxylcytosine in human spermatogenic cells and spermatozoa
- Authors: Efimova O.A.1, Krapivin M.I.1, Parfenyev S.E.2, Mekina I.D.1, Komarova E.M.1, Ishchuk M.A.3, Tikhonov A.V.1, Kogan I.Y.1, Golubeva A.V.2, Daev E.V.2, Gzgzyan A.M.1, Bespalova O.N.1, Pendina A.A.1,2
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Affiliations:
- D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
- Saint Petersburg State University
- D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductologyt"
- Issue: Vol 21, No 1 (2023)
- Pages: 61-74
- Section: Human ecological genetics
- URL: https://journals.rcsi.science/ecolgenet/article/view/132250
- DOI: https://doi.org/10.17816/ecogen120080
- ID: 132250
Cite item
Abstract
Background. The epigenome of gametes is formed under the control of the developmental programme and the influence of environmental factors. How cytosine oxidation patterns are formed and altered in human spermatogenesis remains obscure so far.
The aim of the study was to assess 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) patterns in human spermatogenic cells and spermatozoa.
Materials and Methods. The study was performed on testicular biopsy samples of 10 azoospermic patients and ejaculate samples of 5 sperm donors and 8 patients from infertile couples. The microscope slides were prepared for further indirect immunofluorescence to detect 5fC and 5caC and FISH to determine spermatogenic cell ploidy.
Results. 5fC and 5caC were undetectable in mitotic and meiotic chromosomes of spermatogenic cells, and was present exclusively in some spermatogonia and spermatid interphase nuclei as well as in some ejaculated spermatozoa. The frequency of spermatozoa with 5fC and 5caC varied in a wide range and was higher in patients than in sperm donors (p=0,007, p=0,028). The increase in frequency of spermatozoa with 5fC and 5caC was accompanied with the decrease in frequency of morphologically normal and progressively motile spermatozoa.
Conclusions. 5fC and 5caC are differentially distributed in human spermatogenic cells and spermatozoa. The immunocytochemically detected increase of 5fC and 5caC in individual spermatozoa is most likely induced by oxidative stress caused by effects of internal and external factors rather than developmental programme. The evaluation of 5fC and 5caC in spermatozoa can be potentially used as an additional criterion of ejaculate quality.
Full Text
##article.viewOnOriginalSite##About the authors
Olga A. Efimova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: efimova_o82@mail.ru
ORCID iD: 0000-0003-4495-0983
SPIN-code: 6959-5014
Scopus Author ID: 14013324600
Cand. Sci. (Biol.), head of Laboratory of cytogenetics and cytogenomics of reproduction
Russian Federation, Saint PetersburgMikhail I. Krapivin
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: krapivin-mihail@mail.ru
ORCID iD: 0000-0002-1693-5973
SPIN-code: 4989-1932
Scopus Author ID: 56507166200
junior research associate
Russian Federation, Saint PetersburgSergey E. Parfenyev
Saint Petersburg State University
Email: gen21eration@gmail.com
SPIN-code: 9703-0273
Scopus Author ID: 57190742512
student
Russian Federation, Saint PetersburgIrina D. Mekina
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: irendf@mail.ru
ORCID iD: 0000-0002-0813-5845
SPIN-code: 4682-8590
Scopus Author ID: 7006299063
Cand. Sci. (Biol.), senior research associate
Russian Federation, Saint PetersburgEvgeniia M. Komarova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: evgmkomarova@gmail.com
ORCID iD: 0000-0002-9988-9879
SPIN-code: 1056-7821
Scopus Author ID: 57191625749
Cand. Sci. (Biol.), head of Laboratory of preimplantation development
Russian Federation, Saint PetersburgMariia A. Ishchuk
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductologyt"
Email: mashamazilina@gmail.com
ORCID iD: 0000-0002-4443-4287
SPIN-code: 1237-6373
Scopus Author ID: 24779589100
junior research associate
Russian Federation, Saint PetersburgAndrei V. Tikhonov
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: tixonov5790@gmail.com
ORCID iD: 0000-0002-2557-6642
SPIN-code: 3170-2629
Scopus Author ID: 57191821068
Cand. Sci. (Biol.), research associate
Russian Federation, Saint PetersburgIgor Yu. Kogan
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
SPIN-code: 6572-6450
Scopus Author ID: 56895765600
MD, Dr. Sci. (Med.), Correspondent member RAS, director
Russian Federation, Saint PetersburgArina V. Golubeva
Saint Petersburg State University
Email: AlikovaAV1504@yandex.ru
ORCID iD: 0000-0003-1613-222X
student
Russian Federation, Saint PetersburgEugene V. Daev
Saint Petersburg State University
Email: e.daev@spbu.ru
ORCID iD: 0000-0003-2036-6790
SPIN-code: 8926-6034
Scopus Author ID: 6701779129
Dr. Sci. (Biol.), professor of genetics and biotechnology Department
Russian Federation, Saint PetersburgAleksander M. Gzgzyan
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: agzgzyan@gmail.com
SPIN-code: 6412-4801
Scopus Author ID: 56232643300
MD, Dr. Sci. (Med.)
Russian Federation, Saint PetersburgOlesya N. Bespalova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: shiggerra@mail.ru
SPIN-code: 4732-8089
Scopus Author ID: 57189999252
Dr. Sci. (Med.); deputy director for science
Russian Federation, Saint PetersburgAnna A. Pendina
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State University
Author for correspondence.
Email: pendina@mail.ru
ORCID iD: 0000-0001-9182-9188
SPIN-code: 3123-2133
Scopus Author ID: 6506976983
Cand. Sci. (Biol.), senior research associate
Russian Federation, Saint Petersburg; Saint PetersburgReferences
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