Evaluation of the genotoxic effect of tartrazine using a metabolic activation system in human lymphocyte culture under cytokinetic block conditions
- Authors: Nikitina T.A.1, Konyashkina M.A.1, Ingel F.I.1, Akhaltseva L.V.1
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Affiliations:
- Centre for Strategic Planning and Management of Biomedical Health Risks
- Issue: Vol 21, No 1 (2023)
- Pages: 41-51
- Section: Genetic toxicology
- URL: https://journals.rcsi.science/ecolgenet/article/view/132248
- DOI: https://doi.org/10.17816/ecogen117502
- ID: 132248
Cite item
Abstract
The expansion of the spectrum of use of food additives and, in particular, food dyes (FD), increases the risk of increasing human exposure to genotoxicants. Since in real life, not pure substances with proven genetic safety are in contact with a person, but complex mixtures of unknown composition, even minor impurities in which can become an additional source or modifier of genome instability effects. Of particular concern in this aspect are synthetic FD azo and diazo compounds which can be transformed by human intestinal microflora to some forms of genotoxicants. The purpose of the work is to evaluate the genotoxic effects of 0–2 mg/mL of Tartrazine FD (E102) purchased in a retail network in a micronucleus test on human blood cells cultured under cytokinetic block conditions in parallel in presence and without rat S9 hepatocyte metabolic activation system.
Genotoxic effects were found in cultures without metabolic activation at 0.0000256–0,00064 mg/mL and 0.4 mg/mL of tartrazine, and in the presence of S9 — at 0.0000256 mg/mL, 0,000128 mg/mL and 0.16 mg/mL of tartrazine. For the first time, a dose-dependent suppression of mitotic and proliferative activity of lymphocytes induced by the tested tartrazine sample was revealed, as well as a dose-dependent U-shaped curve in the frequency of apoptosis. The data obtained indicate the presence of genotoxic activity of the studied sample.
We discuss the necessity to create the system for evaluation the genotoxic safety of FD real mixtures from a retail network.
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##article.viewOnOriginalSite##About the authors
Tatyana A. Nikitina
Centre for Strategic Planning and Management of Biomedical Health Risks
Author for correspondence.
Email: TNikitina@cspmz.ru
ORCID iD: 0000-0003-0866-5990
SPIN-code: 9106-5076
biologist, Department of Preventive Toxicology and Biomedical Resear
Russian Federation, MoscowMariya A. Konyashkina
Centre for Strategic Planning and Management of Biomedical Health Risks
Email: MKonyashkina@cspmz.ru
ORCID iD: 0000-0002-8319-1329
SPIN-code: 7559-9045
Scopus Author ID: 8142882800
Cand. Sci. (Med.), research associate of the Department of Preventive Toxicology and Biomedical Research
Russian Federation, MoscowFaina I. Ingel
Centre for Strategic Planning and Management of Biomedical Health Risks
Email: FIngel@cspmz.ru
ORCID iD: 0000-0002-2262-6800
SPIN-code: 1013-7006
Scopus Author ID: 57205760994
Dr. Sci. (Biol.), leading research associate of the Department of Preventive Toxicology and Biomedical Research
Russian Federation, MoscowLyudmila V. Akhaltseva
Centre for Strategic Planning and Management of Biomedical Health Risks
Email: LAhalceva@cspmz.ru
ORCID iD: 0000-0002-3619-3858
SPIN-code: 7049-0003
Scopus Author ID: 57138478700
Cand. Sci. (Biol.), senior research associate of the Department of Preventive Toxicology and Biomedical Research
Russian Federation, MoscowReferences
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