Organization of the endoplasmic reticulum in cells of effective and ineffective pea nodules (Pisum sativum L.)

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Abstract

Background. The endoplasmic reticulum (ER) is the largest membrane-bound organelle, which plays an important role in the functioning of a plant cell and participates in its differentiation.

Materials and methods. Using the methods of transmission electron microscopy, the morphological features and dynamics of structural changes in the ER in symbiotic nodules of pea (Pisum sativum L.) wild-type and mutants blocked at different stages of nodule development were studied.

Results. ER developed from a network of individual tubules in meristematic cells, to a developed network of cisterns around the nucleus and plasmalemma, and a network of granular and smooth tubules accompanying infection structures in colonized and infected cells and symbiosomes in infected cells.

Conclusions. A correlation was found between the level of development of the ER network and the degree of bacteroid differentiation.

About the authors

Anna V. Tsyganova

All-Russia Research Institute for Agricultural Microbiology

Author for correspondence.
Email: isaakij@mail.ru
ORCID iD: 0000-0003-3505-4298
SPIN-code: 9149-5662
http://arriam.ru/departments/laboratoriya-molekulyarnoj-i-kletochnoj-biologii/

Candidate of Biological Sciences, Leading Scientist of the Laboratory of Molecular and Cellular Biology

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Viktor E. Tsyganov

All-Russia Research Institute for Agricultural Microbiology; Saint Petersburg Scientific Center RAS

Email: tsyganov@arriam.spb.ru
ORCID iD: 0000-0003-3105-8689
SPIN-code: 6532-1332
http://arriam.ru/departments/laboratoriya-molekulyarnoj-i-kletochnoj-biologii/

Doctor of Biological Sciences, Head of the Laboratory of Molecular and Cellular Biology, Department of Biotechnology; Senior Scientist of Saint Petersburg Scientific Center RAS

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608; 5, Universitetskaya Nab., St.Petersburg, 199034

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2. Fig. 1. The endoplasmic reticulum in the wild-type symbiotic nodules of P. sativum: a – cell of the meristem; b – cell of the meristem with infection threads; c – cell from the early infection zone; d – cell from the late infection zone; e – infected cell from the early nitrogen fixation zone; and f – mature infected cell from the nitrogen fixation zone. a, b, e, f – Sprint2; c, d – SGE. N – nucleus, V – vacuole, IT – infection thread, ID – infection droplet; arrows indicate profiles of the RER. Bar: 5 µm

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3. Fig. 2. Distribution of the endoplasmic reticulum in infected cells of the wild-type nodules of P. sativum: a – around the nucleus; b – along the plasma membrane; c – along infection threads; d – SER network with the matrix material of infection droplets; e – around individual symbiosomes or groups of symbiosomes; and f – an extension of ER profiles in senescent cells. N – nucleus, M – mitochondrion, V – vacuole, CW – cell wall, IT – infection thread, ITW – infection thread wall, ID – infection droplet, B – bacterium, RB – releasing bacterium, Ba – bacteroid; arrows indicate RER profiles, arrowheads – SER profiles. Bar: a – 2 µm, b–f – 500 nm

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4. Fig. 3. The endoplasmic reticulum in the nodules of P. sativum mutant line SGEFix–-3 (sym26): a – parallel cisternae around the nucleus; b – SER network; c – around individual symbiosomes or groups of symbiosomes; and d – an extension of RER profiles and partial ribosome loss in some cells. N – nucleus, M – mitochondrion, CW – cell wall, Ba – bacteroid; arrows indicate RER profiles, arrowheads – SER profiles. Bar: 500 nm

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5. Fig. 4. The endoplasmic reticulum in the nodules of P. sativum mutant lines SGEFix–-1 (sym40) (a–c) and SGEFix–-2 (sym33-3) (d–f): a – around the vacuole and infection threads and droplets; b – around symbiosomes; c – extension and fragmentation of RER profiles with partial loss of ribosomes; d – along the plasma membrane; e – around symbiosomes; and f – SER network. M – mitochondrion, AG – Golgi apparatus, V – vacuole, CW – cell wall, IT – infection thread, ID – infection droplet, B – bacterium, Ba – bacteroid, * – symbiosome containing several bacteroids surrounded by a common symbiosome membrane; arrows indicate RER profiles, arrowheads – SER profiles. Bar: 500 nm

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6. Fig. 5. The endoplasmic reticulum in the nodules of P. sativum mutant lines SGEFix–-2 (sym33-3) (a, b) and Sprint-2Fix– (sym31) (c, d): a – in a narrow layer of the cytoplasm around the nucleus and infection threads; b – along bacterial free infection droplets; and c – along the plasma membrane; d – around symbiosomes. N – nucleus, M – mitochondrion, CW – cell wall, IT – infection thread, ID – infection droplet, B – bacterium, Ba – bacteroid, * – symbiosome containing several bacteroids surrounded by a common symbiosome membrane; arrows indicate RER profiles. Bar: a, c – 1 µm, b, d – 500 nm

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Copyright (c) 2019 Tsyganova A., Tsyganov V.

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