Histological and ultrastructural nodule organization of the pea (Pisum sativum) mutant sgefix–-5 in the Sym33 gene encoding the transcription factor PsCYCLOPS/PsIPD3

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Abstract

Background. The transcription factor CYCLOPS/IPD3 is a key activator of the organogenesis of symbiotic nodules. Its participation in the development of infection threads and symbiosomes is also shown. In pea, three mutant alleles were identified for this gene (sym33-1 – sym33-3). The phenotypic manifestations of the sym33-3 allele of the SGEFix¯-2 mutant, characterized by a “leaky” phenotype (the formation of two types of nodules: white and pinkish) were the most studied. The sym33-2 allele in the mutant SGEFix¯-5 was described as a strong allele, however, its phenotypic manifestations have not been studied in detail.

Materials and methods. In this study, the histological and ultrastructural nodule organization of the SGEFix¯-5 mutant was analyzed using confocal laser scanning microscopy and transmission electron microscopy.

Results. In the nodules “locked” infection threads were observed, from which no bacteria release into the cytoplasm of the plant cell occurs. In this case, in some infection threads, bacteria were degraded, which may indicate the activation of strong defense reactions in the nodules of the SGEFix¯-5 mutant.

Conclusions. The sym33-2 allele in the mutant SGEFix¯-5 is a strong allele, which triggers the severe defense reactions, when rhizobia are already perceived as pathogens in infection threads.

About the authors

Anna V. Tsyganova

All-Russia Research Institute for Agricultural Microbiology

Email: isaakij@mail.ru
ORCID iD: 0000-0003-3505-4298

Leading Scientist, Laboratory of Molecular and Cellular Biology

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Kira A. Ivanova

All-Russia Research Institute for Agricultural Microbiology

Email: kivanova@arriam.ru
ORCID iD: 0000-0002-9119-065X

Junior Scientist, Laboratory of Molecular and Cellular Biology

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Viktor E. Tsyganov

All-Russia Research Institute for Agricultural Microbiology

Author for correspondence.
Email: tsyganov@arriam.spb.ru
ORCID iD: 0000-0003-3105-8689
SPIN-code: 6532-1332
Scopus Author ID: 7006136325
ResearcherId: Q-5634-2016
http://arriam.ru/departments/laboratoriya-molekulyarnoj-i-kletochnoj-biologii/

Head of the Laboratory, Laboratory of Molecular and Cellular Biology

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

References

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2. Fig. 1. Histological organization of nodules of the mutant line SGEFix–-5 (sym33-2): a – sagittal nodule section; b – colonized cells with infection threads. Merge of single optical sections of differential-interference contrast and red channels (nuclei and bacteria), presented in grayscale. CC –colonized cell, N –nucleus, IT –infection thread, arrows indicate infection threads. Scale bar: a – 50 µm, b –10 µm

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3. Fig. 2. Ultrastructural organization of nodules of the mutant line SGEFix–-5 (sym33-2): а – colonized cells; b – infection threads with thickened walls and intact bacteria in the matrix; c – infection thread with thickened walls and degraded bacteria assembled in clusters; d – infection thread with completely degraded bacteria in the lumen. CC – colonized cell, IT – infection thread, ITW – infection thread wall, CW – cell wall, B – bacterium, DB – degrading bacterium, arrows indicate vesicles, asterisks indicate clusters of bacteria inside the infection thread. Scale bar: а – 10 µm, b-d – 1 µm

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Copyright (c) 2019 Tsyganova A.V., Ivanova K.A., Tsyganov V.E.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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